Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Improved tomato juice culture medium and application thereof

A technology for tomato juice medium and medium is applied in the field of microorganisms, which can solve the problems of inability to infer and calculate, and achieve the effects of low cost, favorable promotion and application, and convenient preparation and operation.

Inactive Publication Date: 2015-01-28
QINGDAO KANGHE FOOD
View PDF3 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] At present, it is still not possible to infer and calculate the medium formula suitable for a certain strain from the basic principles of biochemical reactions. We can only use the basic theories of biochemistry, cell biology, microbiology, etc., and refer to the more suitable ones used by predecessors. The empirical formula of a certain type of strain, combined with the characteristics of the strains used and the product, uses small fermentation equipment such as shake flasks and glass tanks, and selects a more suitable medium according to certain experimental designs and experimental methods

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Improved tomato juice culture medium and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] A kind of improved tomato juice medium, the component of described medium comprises trisaccharide iron (TSI) agar medium, 2.5g / L tomato powder, 6g / L sodium acetate, 2.0g / L dipotassium hydrogen phosphate, 0.5g / L potassium dihydrogen phosphate, 0.6g / L Tween 80, pH value is 6.6-7.0;

[0024] Described trisaccharide iron (TSI) agar medium contains tryptone 15.0g, beef extract powder 10.0g, yeast powder 5.0g, sodium chloride 4.0g, monthly peptone in every liter of trisaccharide iron (TSI) agar medium 5.0g, lactose 20.0g, glucose 1.0g, sucrose 10.0g, ferrous sulfate 0.2g, sodium thiosulfate 0.3g, agar 15.0g, bromocresol violet 0.04g, the rest is distilled water.

[0025] Weigh 62.5g of this product, dissolve it in 1000ml of distilled water by heating, subpackage, autoclave at 121°C for 15 minutes, and set aside.

[0026] Incubate at 36±1°C for 72±3 hours.

[0027]

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to the field of microorganisms and in particular relates to an improved tomato juice culture medium and an application thereof. The improved tomato juice culture medium comprises the following components: a triple sugar iron (TSI) agar culture medium, 2.5g / L of tomato powder, 6g / L of sodium acetate, 2.0g / L of dipotassium phosphate, 0.5g / L of monopotassium phosphate and 0.6g / L of Tween 80, and pH value of the improved tomato juice culture medium is 6.6-7.0; and one litre of triple sugar iron (TSI) agar culture medium contains the following components: 15.0g of tryptone, 10.0g of beef extract powder, 5.0g of yeast powder, 4.0g of sodium chloride, 5.0g of proteose peptone, 20.0g of lactose, 1.0g of glucose, 10.0g of cane sugar, 0.2g of ferrous sulfate, 0.3g of sodium thiosulfate, 15.0g of agar, 0.04g of bromcresol purple and balance of distilled water. The improved tomato juice culture medium is simple in composition, easy to prepare and operate, low in cost and beneficial to popularization and application; bacteria grow quickly, and mutation rate is low; and observation and analysis are facilitated.

Description

technical field [0001] The invention belongs to the field of microorganisms, and in particular relates to an improved tomato juice culture medium and its application. Background technique [0002] The diversity and variability among microorganisms in the world and the ecological complexity of species habitats have brought about the breadth, complexity and permanence of exploring and understanding microorganisms. A species of microorganism is a unique gene pool. The metabolism of microorganisms is an intricate life activity. In the long-term biological evolution, a complete and unified sensitive regulatory system has been formed. Depending on this regulatory system, various metabolic activities are strictly controlled. . Methodical and coordinated and adapts flexibly to circumstances. Different culture media, due to different media formulations, affect its growth and variation. [0003] Medium is an artificially prepared nutrient for the growth and maintenance of microorga...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12Q1/14C12Q1/04C12R1/445C12R1/23C12R1/46C12R1/01
Inventor 刘振宇李鹏
Owner QINGDAO KANGHE FOOD
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com