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Glycolated serum albumin detection reagent and application thereof

A technology of serum albumin and detection reagents, which is applied in the direction of analyzing materials through chemical reactions, material analysis through observing the influence of chemical indicators, color/spectral characteristic measurement, etc., which can solve the problem of poor accuracy of detection results and repeated Low sensitivity and other problems, to achieve high precision, high recovery rate, and ensure the effect of accuracy

Inactive Publication Date: 2016-01-13
GUANGZHOU KINGMED DIAGNOSTICS CENT
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] In order to overcome the defects of poor accuracy and low repeatability of the detection results of the glycated serum albumin detection reagent in the prior art, the purpose of the present invention is to provide a glycated serum albumin detection reagent and its application to solve the above problems

Method used

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Experimental program
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Effect test

Embodiment 1

[0033] Embodiment 1, Glycated serum albumin detection reagent

[0034] Glycated serum albumin detection reagents include reagent R1, reagent R2 and reagent R3, and the reagent R1 includes the following components:

[0035] MES buffer 20mmol / L, glycated amino acid oxidase 2U / ml, protease 5U / ml, sodium chloride 25μmol / L and proclin3000.1g / L;

[0036] The reagent R2 comprises the following components:

[0037] MES buffer 20mmol / L, N,N-bis(4-sulfobutyl)-3-methylaniline disodium salt 1mmol / L, 4-aminoantipyridine 5mmol / L, peroxidase 2U / ml , ethylene glycol 5mg / ml, alkyl polyglucoside 10mmol / L and proclin3000.1g / L;

[0038] The reagent R3 comprises the following components:

[0039]Acetate buffer with a pH value of 4.5 20mmol / L, bromocresol violet 2mmol / L, alkyl polyglucoside 30mmol / L and proclin3000.1g / L.

Embodiment 2

[0040] Embodiment 2, Glycated serum albumin detection reagent

[0041] Glycated serum albumin detection reagents include reagent R1, reagent R2 and reagent R3, and the reagent R1 includes the following components:

[0042] MES buffer 40mmol / L, glycated amino acid oxidase 8U / ml, protease 6U / ml, sodium chloride 30μmol / L and proclin3000.2g / L;

[0043] The reagent R2 comprises the following components:

[0044] MES buffer 40mmol / L, N,N-bis(4-sulfobutyl)-3-methylaniline disodium salt 4mmol / L, 4-aminoantipyridine 10mmol / L, peroxidase 4U / ml , ethylene glycol 8mg / ml, alkyl polyglucoside 20mmol / L and proclin3000.2g / L;

[0045] The reagent R3 comprises the following components:

[0046] Acetate buffer solution with a pH value of 4.9 is 30mmol / L, bromocresol purple is 5mmol / L, alkyl polyglucoside is 50mmol / L and proclin3000.2g / L.

Embodiment 3

[0047] Embodiment 3, Glycated serum albumin detection reagent

[0048] Glycated serum albumin detection reagents include reagent R1, reagent R2 and reagent R3, and the reagent R1 includes the following components:

[0049] MES buffer 60mmol / L, glycated amino acid oxidase 10U / ml, protease 10U / ml, sodium chloride 50μmol / L and preservative 0.3g / L;

[0050] The reagent R2 comprises the following components:

[0051] MES buffer 60mmol / L, N,N-bis(4-sulfobutyl)-3-methylaniline disodium salt 8mmol / L, 4-aminoantipyridine 20mmol / L, peroxidase 6U / ml , polyol 10mg / ml, alkyl polyglucoside 30mmol / L and preservative 0.3g / L;

[0052] The reagent R3 comprises the following components:

[0053] Acetate buffer solution with a pH value of 5.5 is 50mmol / L, bromocresol violet is 10mmol / L, alkyl polyglucoside is 80mmol / L and preservative is 0.3g / L.

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Abstract

The invention belongs to the technical field of medical examination, and specifically relates to a glycolated serum albumin detection reagent and application thereof. The reagent comprises a reagent R1, a reagent R2 and a reagent R3, wherein the reagent R1 is mainly composed of an MES buffer solution, glycolated amino-acid oxidase, protease, sodium chloride and a preservative; the reagent R2 is mainly composed of the MES buffer solution, N,N-bis(4-sulfobutyl)-3-methylaniline disodium salt, 4-aminoantipyrine, peroxidase, polyol, alkyl polyglucoside and the preservative; and the reagent R3 is mainly composed of an acetate buffer solution, bromocresol purple, alkyl polyglucoside and the preservative. The glycolated serum albumin detection reagent provided by the invention has the advantages of strong anti-interference ability, high precision, good repeatability of determination results, and high detection reagent stability, which facilitates clinical application of the glycolated serum albumin detection reagent.

Description

technical field [0001] The invention belongs to the technical field of medical testing, and in particular relates to a detection reagent for glycosylated serum albumin and an application thereof. Background technique [0002] Glycated serum albumin (glyecatedalbumin, GA) is a ketamine compound formed by the combination of glucose and albumin in plasma through non-enzymatic glycosylation reaction. The glycosylated serum albumin value can reflect the average blood sugar level of diabetic patients 2-3 weeks before the test, which is consistent with the gold standard of blood sugar monitoring, HbA IC In contrast, GA can reflect the short-term fluctuation of blood sugar, and can more accurately reflect the blood sugar of diabetic patients. Good blood sugar control can effectively delay the occurrence and development of diabetes and chronic complications. Therefore, the determination of glycosylated serum albumin is of great significance in the diagnosis, short-term monitoring a...

Claims

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Application Information

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IPC IPC(8): G01N21/31G01N21/78
Inventor 燕启江郭周萍庞柳刘菲菲
Owner GUANGZHOU KINGMED DIAGNOSTICS CENT
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