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Reagent for detecting rice new-old degree and its detecting method

A technology for detection reagents and detection methods, which is applied in the direction of testing food, testing starch contaminants, and testing materials for materials, etc., can solve the problems of high cost, difficult popularization and application, inconvenient operation, etc., and achieves simple manufacturing process, low reagent cost, Actionable fast effects

Inactive Publication Date: 2005-07-20
江苏省无锡江大大学科技园有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Using the above-mentioned detection means and detection methods has high cost and inconvenient operation, and can only be used in scientific research units, so it is difficult to popularize and apply; in daily life, it is difficult for ordinary people to accept

Method used

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  • Reagent for detecting rice new-old degree and its detecting method

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Comparison scheme
Effect test

Embodiment 1

[0013] In the embodiment of the present invention, nitrazine yellow is 0.4% by weight, p-nitrophenol is 8% by weight, bromocresol green is 12% by weight, bromocresol purple is 15% by weight, hematoxylin or methylbenzene The base red is: 10% by weight is added together into a container containing 89.5% by weight of solvent alcohol to dissolve, the alcohol concentration is: 89.8% by weight, and then mixed uniformly to prepare a finished reagent stock solution. When testing and using, take 5 wt% of the reagent stock solution and add it to a reagent bottle (container) filled with 95 wt% distilled water diluent, mix it evenly into a liquid reagent, then weigh 2 grams of rice sample and add it to 4ml liquid reagent, and mix it evenly , The reaction time is 3 minutes by the liquid reagent and the rice. Observe the color of the rice and the solution, and judge the freshness of the rice from the color change of the reagent and the rice. The freshness and color of the rice correspond to Dar...

Embodiment 2

[0015] In the embodiment of the present invention, chlorophenol red is 4.8% by weight, p-nitrophenol is 3.95% by weight, bromocresol green or bromothymol blue is 5.85% by weight, and bromocresol purple is 7.45% by weight , Cresyl red: 4.95 wt% is added to a container containing 89.75 wt% of solvent alcohol to dissolve, the alcohol concentration is: 90.8 wt%, and then mixed uniformly to prepare a finished reagent stock solution. When testing, take 7.5% by weight of the reagent stock solution and add it to a reagent bottle (container) filled with 92.5% by weight of distilled water diluent, mix it into a liquid reagent, and then weigh 2 grams of rice sample and add it to 4ml of the liquid reagent. , The reagent reacts with the rice (standstill) for 12 minutes, the liquid reagent reacts with the rice, observe the color of the rice and the solution, and judge the freshness of the rice from the color change of the reagent and the rice. The rice and the color correspond to dark green , G...

Embodiment 3

[0017] In the embodiment of the present invention, 10% by weight of hematoxylin, 0.1% by weight of p-nitrophenol, 0.3% by weight of resorcinol blue, 0.1% by weight of bromocresol purple, and 0.1% by weight of bromophenol red are used. The weight% is added to a container containing 80 weight% of solvent alcohol for dissolution, the alcohol concentration is 89.8 weight %, and then mixed uniformly to prepare a finished reagent stock solution. When testing and using, take 10% by weight of the reagent stock solution and add it to a reagent bottle (container) containing 90% by weight distilled water diluent, mix it evenly into a liquid reagent, then weigh 2 grams of rice sample and add it to 4ml of liquid reagent, and mix it evenly Reaction time (standstill): 15 minutes, pour out the solution, react with the liquid reagent and rice, observe the color of the tested rice, and judge the rice freshness or whether the rice samples have different freshness rice mixed together, The condition a...

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Abstract

The invention relates to a rice metabolism detecting reagent and method, concretely used in the condition of detecting rice metabolism. It mainly takes and dissolves nitrazine yellow / chlorophenol red, p-nitrophenol, bromocresol green / bromothymol blue / resorcinol blue, bromocresol purple, and hematoxylin / cresol red / bromophenol red / methyl red into alcohol, and mixing them uniformly to obtain finished product of raw reagent liquid. As detecting, taking a raw reagent liquid and adding distilled water to dilute the raw reagent liquid so as to form a liquid reagent, adding rice to the liquid reagent to react, observing the colors of rice and solution, judging the metalolism degree of the rice detected, the metabolism situation and color of the rice corresponds to green and passes through yellow and salmon pink into red region, where the green expresses new rice, yellow expresses old rice and salmon pink expresses deeply aged rice. The reagent of the invention is lower-cost and has simple manufacturing process; the operation is quick, accurate and convenient; the detecting method is simple, able to be used in both qualitative detection and quantitative detection.

Description

Technical field [0001] The invention relates to a detection reagent for the freshness of rice and a detection method thereof, in particular to the detection of the freshness of rice, belonging to the technical field of food detection methods. Background technique [0002] Rice is the main food in people's lives. Fresh rice is rich in nutrients and has a good taste, which is deeply loved by people. Old rice has a poor taste, and protein, fatty acids, carbohydrates, etc. will be oxidized and deteriorated over time. Before the present invention was made, in the prior art, the current judging of the freshness of freshly-aged rice was mainly carried out by professional and technical personnel using professional instruments and equipment; the use method usually uses optical, chemical, gas-phase, chromatography and other instruments to analyze rice Quantitative methods for protein SH group and SS group, fatty acid, volatile carbonyl compounds, and volatile sulfide content, as well as me...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/78G01N33/02G01N33/10
Inventor 毛忠贵吕季璋卢泽民王正刚周望岩
Owner 江苏省无锡江大大学科技园有限公司
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