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Lactose bile salt fermentation culture medium and application thereof

A technology of fermentation medium and lactose bile salt, which is applied in the field of microorganisms, can solve problems such as inability to infer and calculate, and achieve the effects of low cost, easy observation, and favorable promotion and application

Inactive Publication Date: 2015-01-28
QINGDAO KANGHE FOOD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, it is still not possible to infer and calculate the medium formula suitable for a certain strain from the basic principles of biochemical reactions. We can only use the basic theories of biochemistry, cell biology, microbiology, etc., and refer to the more suitable ones used by predecessors. The empirical formula of a certain type of strain, combined with the characteristics of the strains used and the product, uses small fermentation equipment such as shake flasks and glass tanks, and selects a more suitable medium according to certain experimental designs and experimental methods

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] A lactose bile salt fermentation medium, the components of the medium include EC broth, 5.0g / L ox bile salt, 0.065g / L bromocresol purple, 1.0g / L ferric citrate, 15.0g / L Agar, pH 7.3-7.5.

[0024] The EC broth contains 20.0 g of tryptone, 10.0 g of lactose, 5.0 g of sodium chloride, 4.0 g of dipotassium hydrogen phosphate per liter of the EC broth, and the rest is distilled water.

[0025] Weigh 35g of this product and add 1000ml of distilled water, stir and heat to dissolve, divide into test tubes, 10mL per tube, put into a small inverted tube, autoclave at 115°C for 15min, cool rapidly, and set aside.

[0026] The prepared liquid medium should be stored at 2-8°C and should be protected from direct light exposure. The dry medium should be placed in a dark and dry place at a storage temperature of 2-30°C.

[0027] Incubate at 37°C for 20-24 hours:

[0028] Quality Control Strains

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PUM

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Abstract

The invention belongs to the microbial field, especially relates to a lactose bile salt fermentation culture medium, and is characterized in that the culture medium consists of an EC broth, 5.0 g / L of a bile salt from ox, 0.065 g / L of bromcresol purple, 1.0 g / L of ferric citrate, and 15.0 g / L of agar, wherein the pH value is 7.3-7.5; the EC broth contains 20.0 g of tryptone, 10.0 g of lactose, 5.0 g of sodium chloride, 4.0 g of dipotassium hydrogen phosphate, and the balance distilled water in each liter of the EC broth. The culture medium has the advantages of simple formula, convenient preparation operation, low cost, and favorable popularization and application; bacteria grow rapidly, and the mutation rate is low; and observation and analysis are convenient.

Description

technical field [0001] The invention belongs to the field of microorganisms, and in particular relates to a lactose bile salt fermentation medium and application thereof. Background technique [0002] The diversity and variability among microorganisms in the world and the ecological complexity of species habitats have brought about the breadth, complexity and permanence of exploring and understanding microorganisms. A species of microorganism is a unique gene pool. The metabolism of microorganisms is an intricate life activity. In the long-term biological evolution, a complete and unified sensitive regulatory system has been formed. Depending on this regulatory system, various metabolic activities are strictly controlled. . Methodical and coordinated and adapts flexibly to circumstances. Different culture media, due to different media formulations, affect its growth and variation. [0003] Medium is an artificially prepared nutrient for the growth and maintenance of micro...

Claims

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Application Information

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IPC IPC(8): C12Q1/04C12R1/19
Inventor 刘振宇李鹏
Owner QINGDAO KANGHE FOOD
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