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Electrochemical sensor for simultaneous detection of DNA bases

An electrochemical and sensor technology, applied in the field of electrochemical detection of four DNA bases, can solve the problems of cumbersome operation, sample turbidity, poor stability, etc., and achieve the effect of safe operation and less pollution

Inactive Publication Date: 2014-04-30
NORTHEAST NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] The purpose of the present invention is to solve the problem that the existing electrochemical sensors are difficult to realize simultaneous sensitive analysis and stable detection of four DNA bases. The existing methods are susceptible to sample turbidity, hemolysis, jaundice interference, complex and expensive instruments, and cumbersome operations. problems, as well as the narrow potential window of existing electrochemical sensors, low response current to pyrimidine bases, poor stability, etc., and an electrochemical sensor for simultaneous detection of DNA bases is provided

Method used

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  • Electrochemical sensor for simultaneous detection of DNA bases
  • Electrochemical sensor for simultaneous detection of DNA bases
  • Electrochemical sensor for simultaneous detection of DNA bases

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specific Embodiment approach 1

[0025] Specific embodiment one: a kind of electrochemical sensor for the simultaneous detection of DNA bases of the present embodiment is characterized in that it is carried out according to the following steps:

[0026] Step 1: Ultrasonic disperse the graphene oxide powder prepared by the Hummer method in double-distilled water for 10-15 minutes to obtain a graphene oxide suspension with a concentration of 0.2-0.5 mg / mL; add bromocresol purple to a concentration of 0.01 M NaNO 3 dispersed in the solution to obtain a concentration of 1.0×10 -3 ~2.0×10 -3 The bromocresol purple solution of M;

[0027] Step 2: Take 5-10 μL of the graphene oxide suspension obtained in Step 1, drop-coat it on the surface of the glassy carbon electrode, place it under an infrared lamp to dry, and cool to room temperature after drying to obtain a graphene oxide-modified glassy carbon electrode; The glassy carbon electrode modified by graphene oxide was placed in PBS solution with pH=5~7 for const...

specific Embodiment approach 2

[0033] Embodiment 2: This embodiment differs from Embodiment 1 in that: the ultrasonic dispersion in double-distilled water described in Step 1 lasts for 12-15 minutes. Others are the same as in the first embodiment.

specific Embodiment approach 3

[0034] Embodiment 3: This embodiment differs from Embodiment 1 or Embodiment 2 in that: the ultrasonic dispersion in double distilled water described in step 1 is 15 minutes. Others are the same as in the first or second embodiment.

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Abstract

The invention discloses an electrochemical sensor for simultaneous detection of DNA bases, relates to the electrochemical detection of DNA and solves the problems that an existing electrochemical sensor is difficult to realize simultaneous sensitivity analysis and stability detection of four DNA bases, the electrochemical sensor is easily polluted by a sample, complex and expensive, and complicated to operate in an existing method, a potential window is narrow, the response current to a pyrimidine base is low and the stability is poor. The bases are detected simultaneously through the following steps by the electrochemical sensor: I, preparing an oxidized graphene and bromcresol purple solution; II, preparing a graphene-modified electrode; III, preparing a bromcresol purple / graphene composite modified electrode; IV, detecting an electrochemical signal of a four-base mixed solution by using the composite modified electrode, and analyzing and determining a peak position where the electrochemical signal is located; V, carrying out electrochemical detection on the base mixed solution to obtain a differential pulse voltammetry diagram with the change of concentration, and establishing an electrochemical signal change law diagram of the four DNA bases. The invention is applied to the field of electrochemical sensors.

Description

technical field [0001] The invention relates to the electrochemical detection of deoxyribonucleic acid (DNA), especially the electrochemical detection of four DNA bases. Background technique [0002] Deoxyribonucleic acid (DNA) is the main chemical component of chromosomes. Its basic composition is deoxyribonucleotides, which are composed of phosphoric acid, D-2 deoxyribose and bases. The bases include Adenine, Guanine, Thymine and Cytosine. As the carrier of genetic information, DNA is closely related to the normal activities of life such as reproduction, heredity, and cell differentiation. At the same time, the oxidative damage and mutation of DNA are closely related to abnormal activities of life such as tumors, genetic diseases and metabolic diseases. Oxidative damage to DNA usually occurs on bases, and the degree of DNA damage can be predicted by detecting changes in bases in body fluids, indicating the occurrence of certain diseases, and it is of great significance f...

Claims

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Application Information

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IPC IPC(8): G01N27/26
Inventor 袁星武冬梅朱晓琳李锦莲
Owner NORTHEAST NORMAL UNIVERSITY
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