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Legionnella selective separation medium

A separation culture medium and selective technology, applied in the field of culture medium, can solve the problems of expensive culture medium, unfavorable popularization and application, and high detection cost, and achieve the goal of improving the positive rate of Legionella isolation and culture, facilitating popularization and application, and increasing the positive rate Effect

Active Publication Date: 2010-02-10
贵州金域医学检验中心有限公司
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  • Abstract
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  • Claims
  • Application Information

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Problems solved by technology

[0002] At present, the legionella isolation medium recognized at home and abroad are BCYEα-CCVC, BCYEα-GVPC and BCYEα-GPVA produced by bioMérieux in France, OXOID in the United Kingdom, BD in the United States, DIFCO, etc. [1] , most of these media are expensive (18-25 yuan / plate), and when they are widely used in hospital clinical and health and epidemic prevention departments, the detection cost is high
And the positive rate of Legionella isolation and culture is generally 35.1% to 65.0%. [2-3] , the positive rate is relatively low
Therefore existing legionella isolation culture medium is unfavorable for clinical and sanitation and epidemic prevention department popularization and application

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Legionella selective isolation culture medium, the component parts by weight that it comprises are as follows:

[0036] Base fluid:

[0037] Yeast powder 9~11

[0038] Agar 12~14

[0039] Toner 1.5~2.3

[0040] ACES9~11

[0041] a-ketoglutaric acid 0.8~1.2

[0042] Iron pyrophosphate 0.22~0.27

[0043] protein solution:

[0044] Glycine 2.8~3.2

[0045] L-cysteine ​​0.4~0.6

[0046] Albumin 0.08~0.12

[0047] Dye solution:

[0048] Bromocresol purple 0.008~0.012

[0049] Bromophenol blue 0.008~0.012

[0050] Antibiotic solution:

[0051] Vancomycin 0.0008~0.0012

[0052] Polymyxin B 0.007~0.009

[0053] Cycloheximide 0.007~0.009

[0054] The preparation steps are as follows:

[0055] (1) Yeast powder, agar, carbon powder, ACES and a-ketoglutaric acid in the base liquid are weighed according to the above parts by weight and placed in a container, add 930-980 parts of water, and adjust the pH value to 6.85 with KOH; boil 1 minute, then high temperature st...

Embodiment 2

[0061] Legionella selective isolation culture medium (BCYEα+DGVPC), the component parts by weight that comprise are as follows:

[0062] Base fluid:

[0063] yeast powder 10

[0064] Agar 13

[0065] Toner 2.0

[0066] ACES 10

[0067] a-ketoglutarate 1.0

[0068] Iron pyrophosphate 0.25

[0069] protein solution:

[0070] Glycine 3.0

[0071] L-cysteine ​​0.5

[0072] Albumin 0.1

[0073] Dye solution:

[0074] Bromocresol Violet 0.01

[0075]Bromophenol blue 0.01

[0076] Antibiotic solution:

[0077] Vancomycin 0.001

[0078] Polymyxin B 0.008

[0079] Cycloheximide 0.008

[0080] The preparation steps are as follows:

[0081] (1) Yeast powder, agar, carbon powder, ACES and α-ketoglutaric acid in the base liquid are weighed and placed in a container according to the above parts by weight, add 950 parts of water, and adjust the pH value to 6.85 with KOH; boil 1 minute, then autoclave (15 lbs) at 121°C for 15 minutes; dissolve ferric pyrophosphate in 10 parts...

Embodiment 3

[0087] Legionella selective isolation culture medium, the component parts by weight that it comprises are as follows:

[0088] Base fluid:

[0089] yeast powder 8

[0090] Agar 10

[0091] Toner 1.5

[0092] ACES 8

[0093] a-ketoglutarate 0.5

[0094] Iron pyrophosphate 0.15

[0095] protein solution:

[0096] Glycine 2.5

[0097] L-cysteine ​​0.3

[0098] Albumin 0.05

[0099] dye solution

[0100] Bromocresol Violet 0.005

[0101] Bromophenol Blue 0.005

[0102] antibiotic solution

[0103] Vancomycin 0.0005

[0104] Polymyxin B 0.006

[0105] Cycloheximide 0.006

[0106] The preparation steps are as follows:

[0107] (1) Yeast powder, agar, carbon powder, ACES and a-ketoglutaric acid in the base liquid are weighed according to the above parts by weight and placed in a container, add 900 parts of water, adjust the pH value to 6.85 with KOH; boil for 1 minute , and then sterilized at 121°C for 15 minutes; dissolved ferric pyrophosphate in 8 parts of water, a...

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Abstract

The invention discloses a legionnella selective separation medium. Dyes such as bromothymol blue and bromocresol purple are added to the basic formulation of the basic medium of legionnella BCYE alphaand legionnella selective antibiotics of glycin, vancocin, polymyxin B and actinone are also added. The added dyes produce pigment in the growth process of legionnella; the shape and the color of a colony are beneficial to identifying and distinguishing a target bacterium of the legionnella; the concentrations of the selective antibiotics are added and regulated, the inhibiting capability to non-legionnella and microbial class is enhanced and the inhibiting performance for the legionnella is reduced, thereby enhancing the positive rate for the separation culture of the legionnella; the detection cost is reduced and the legionnella selective separation medium is economic and practical and is convenient for popularization and application for clinical and health and epidemic prevention departments.

Description

Technical field: [0001] The invention relates to a culture medium, specifically a selective separation culture medium for Legionella that is suitable for the isolation and culture of Legionella in environmental and clinical specimens, can reduce the contamination of miscellaneous bacteria, and improve the positive rate of Legionella isolation and culture. Background technique: [0002] At present, the legionella isolation medium recognized at home and abroad are BCYEα-CCVC, BCYEα-GVPC and BCYEα-GPVA produced by bioMérieux in France, OXOID in the United Kingdom, BD in the United States, DIFCO, etc. [1] , most of these media are expensive (18-25 yuan / plate), and when they are widely used in hospital clinical and health and epidemic prevention departments, the detection cost is high. And the positive rate of Legionella isolation and culture is generally 35.1% to 65.0%. [2-3] , the positive rate is relatively low. Therefore existing legionella isolation culture medium is unfav...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12Q1/04C12R1/01
Inventor 朱庆义胡朝晖詹晓勇王娟张远志
Owner 贵州金域医学检验中心有限公司
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