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Fecal coliform culture medium and application thereof

A technology of fecal coliform and chromogenic culture medium, which is applied in the field of medical testing, can solve the problems of low detection accuracy and dependence on subjective judgment, and achieve the effect of short detection cycle, short culture time and good specificity

Inactive Publication Date: 2016-12-21
郑州点石生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The invention provides a culture medium for fecal coliform bacteria, which solves the problem of relying on the subjective judgment of inspectors and the problem of low detection accuracy in the detection of fecal coliform bacteria in existing cosmetics

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] This embodiment provides a fecal coliform color developing medium, which is composed of the following components: peptone 15g / L, liver extract powder 3g / L, lactose 10g / L, bile salt 1.0g / L, dodecane Sodium sulfate 0.3 / L, bromocresol violet 0.05g / L, lecithin 2g, Tween 8013g / L, the rest are deionized water, pH 7.4±0.2.

[0020] Among them, the bile salts of pigs are bile salts.

[0021] How to use the above medium:

[0022] 1. Weigh 75.0 g of this culture medium, add 1 L of deionized water, stir and heat to boil until completely dissolved, and dispense into test tubes with catheters. Each tube is filled with 10 ml of culture medium, sterilized by high pressure steam at 115°C for 20 minutes, and used for later use.

[0023] 2. Take 10ml of cosmetics with appropriate dilution (containing fecal coliform), add it to a test tube containing culture medium, mix well, incubate at 44±0.5°C for 24-48 hours, and put the activated Staphylococcus aureus. , Proteus, Proteus, and Bacil...

Embodiment 2

[0027] This embodiment provides a fecal coliform color developing medium, which is composed of the following components: peptone 10g / L, liver extract powder 4g / L, lactose 5g / L, bile salt 1.5g / L, dodecane Sodium sulfate 0.2 / L, bromocresol violet 0.06g / L, lecithin 1g, Tween 8015g / L, the rest are deionized water, pH 7.4±0.2.

[0028] Among them, the bile salts of pigs are bile salts.

[0029] How to use the above medium:

[0030] 1. Weigh 75.0 g of this culture medium, add 1 L of deionized water, stir and heat to boil until completely dissolved, and dispense into test tubes with catheters. Each tube is filled with 10 ml of culture medium, sterilized by high pressure steam at 115°C for 20 minutes, and ready for use.

[0031] 2. Take 10ml of cosmetics with appropriate dilution (without fecal coliform), add it to a test tube containing culture medium, mix well, incubate at 44±0.5°C for 24-48 hours, and put the activated golden yellow grapes. Coccus, Proteus, Proteus, and Bacillus ...

Embodiment 3

[0035] This embodiment provides a fecal coliform coloring medium, which is composed of the following components: peptone 20g / L, liver extract powder 2g / L, lactose 15g / L, bile salt 0.5g / L, dodecane Sodium sulfate 0.4g / L, bromocresol violet 0.03g / L, lecithin 3g, Tween 8010g / L, the rest are deionized water, pH 7.4±0.2.

[0036] Among them, the bile salts of pigs are bile salts.

[0037] How to use the above medium:

[0038] 1. Weigh 75.0 g of this culture medium, add 1 L of deionized water, stir and heat to boil until completely dissolved, and dispense into test tubes with catheters. Each tube is filled with 10 ml of culture medium, sterilized by high pressure steam at 115°C for 20 minutes, and ready for use.

[0039] 2. Take 10ml of cosmetics with appropriate dilution (containing fecal coliform), add it to a test tube containing culture medium, mix well, incubate at 44±0.5°C for 24-48 hours, and put the activated Staphylococcus aureus. , Proteus, Proteus, and Bacillus cereus w...

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Abstract

The invention discloses a fecal coliform chromogenic culture medium and application thereof, belonging to the technical field of medical inspection. The fecal coliform chromogenic culture medium is composed of 10-20 g / L peptone, 2-4 g / L animal tissue digested substance, 5-15 g / L lactose, 0.5-1.5 g / L bile salt, 0.2-0.4 g / L lauryl sodium sulfate, 0.03-0.06 g / L bromocresol purple, 1-3g of lecithin, 10-15 g / L Tween 80 and the balance of deionized water. The pH value is 7.4+ / -0.2. The culture medium is used for detecting fecal coliform in cosmetics, and has the advantages of high detection sensitivity and high specificity; the culture medium can be used for identifying the strain according to the color, so the detection period is short, and the culture medium is suitable for treating massive samples; and the culture medium can easily implement industrialized production, can be used for carrying out comprehensive, systematic and accurate detection and preliminary identification on fecal coliform in cosmetics, and provides a new way for cosmetic detection.

Description

technical field [0001] The invention relates to the technical field of medical testing, in particular to a fecal coliform culture medium and application thereof. Background technique [0002] Fecal coliforms are a subspecies of the total coliform; fecal coliform bacteria are derived from the feces of humans and warm-blooded animals. The detection of fecal coliforms indicates that the cosmetic has been contaminated with feces, and there may be dangers of other enteric pathogens or pathogens such as parasites. It is considered that bacteria in the total coliform group often exist in the natural environment (water and soil) in addition to living in the gut. Escherichia coli (E.coli) has the largest number of bacteria in the group of fecal coliform bacteria, and is an ideal indicator bacteria. However, the procedures for correct identification of this bacteria are complicated. Therefore, fecal large intestine is used in cosmetic inspection. The flora is listed as an important ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/04
CPCC12Q1/04
Inventor 李杰
Owner 郑州点石生物技术有限公司
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