Nutrient solution for detecting bacterial vaginosis, kit and detection method

Abstract

The invention discloses a nutrient solution for detecting bacterial vaginosis. Each 100ml of nutrient solution contains the following raw materials: 0.1-1g of beef heart powder, 0.5-5g of tryptone, 0.1-2g of glucose, 0.1-1g of sodium chloride, 0.1-1g of bromocresol purple sodium, 5-20ml of horse serum, 0.0001-0.001g of neomycin sulfate and 0.0001-0.001g of nystatin. The invention further discloses a kit for detecting bacterial vaginosis and a detection method. The detection kit is stable in quality; the detection method is simple in operation and accurate in result. The kit has the functions of target cultivation and simultaneous detection of the sensitivity of a drug, the detection method is completed in one step within 6-24h, the success rate is high, the drug sensitivity result is reliable, clinical accurate diagnosis of the bacterial vaginosis and the drug sensitivity test can be met, a reference foundation is provided for a clinician in timely accurate diagnosis and reasonable use of the drug, and clinical abuse of antibiotics is avoided.

Description

technical field [0001] The invention belongs to the technical field of microbial diagnosis, and in particular relates to a culture solution, a kit and a detection method for detecting bacterial vaginosis. Background technique [0002] At present, the detection of bacterial vaginosis is mainly based on the traditional method, and microscopic observation is commonly used. The gold standard method is culture, separation and identification, but the steps are complicated, the detection cycle is long, and the separation rate is not high. The whole process takes about 4-7 days. It is cumbersome and can no longer meet the needs of rapid and convenient detection, and when there are many bacteria in the sample, it will affect the identification of the real target bacteria. [0003] The traditional culture medium basically uses CDC anaerobic agar medium, which uses agar as a coagulant and is suitable for solid plate culture, but its nutrient composition is complex, the culture time is ...

AI Technical Summary

Problems solved by technology

[0002] At present, the detection of bacterial vaginosis is mainly based on the traditional method, and microscopic observation is commonly used. The gold standard method is culture, separation and identification, but the steps are complicated, the detection cycle is long, and the separation rate is not high. The whole process takes about 4-7 days. It is cumbersome and can no longer meet the needs of rapid and convenient detection, and when there are many bacteria in the sample, it will affect the identification of the real target bacteria

[0003] The traditional culture medium basically uses CDC anaerobic agar medium, which uses agar as a coagulant and is suitable for solid plate culture, but its nutrient composition is complex, the culture time is long, and it takes 24 to 48 hours. It is inconvenient for clinical use and is not suitable for bacterial growth. The culture of anaerobic bacteria such as Gardnerella infected by vaginitis; and it does not have the function of targeted culture, and there are often more miscellaneous bacteria growing, which will cause great obstacles to the next step of colony screening. The colonies need to be manually selected and cultivated before purification and identification , higher requirements on the quality of operators, and more cumbersome steps

Before use, it needs to be sterilized and then poured into a plate. The steps are complicated. The finished plate can only be stored at a temperature of 2-8°C, and the validity period is only 7 days. At the same time, the traditional drug susceptibility testing method must be separated and identified for each pathogen. This kind of drug susceptibility test has certain requirements on the quality of the culture medium, such as thickness, surface humidity and hardness, etc. will affect the drug migration. The accuracy of the measurement of the inhibition zone is related to the subjective consciousness of the operator, and the judgment range is large, which is prone to human error , the whole experiment process is complicated and takes a long time, it takes 48-72 hours, and the identification process requires high-quality personnel

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