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Bacillus subtilis, its combination preparation and method for preparing combination preparation

A technology of Bacillus subtilis and compound preparation, applied in the direction of bacteria, etc., can solve the problems of no antagonism, no antibiotic resistance, etc., and achieve the effects of reducing drug residues, improving sensitivity, and improving safety

Inactive Publication Date: 2005-06-29
烟台绿云生物工程研究院有限公司
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Problems solved by technology

But its weak point is that there is no antagonism to Salmonella D enteriditis, Staphylococcus aureus, StreptococcusPyogenes, Proteus mirabili, Proteus Vulgaris, Pseudomonas aeruginosa, Klebsillapneumoniae, and Bacillus subtilis BS-3 does not have antibiotic resistance, so whether the preparation can be used with antibiotics Simultaneous use is under study

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  • Bacillus subtilis, its combination preparation and method for preparing combination preparation

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[0081] Composition of compound preparations. Based on Bacillus subtilis LY-35 and E. Coli, Salmonella D enteriditis, Staphylococcus aureus, Streptococcus Pyogenes, Proteus mirabili, Proteus Vulgaris, Pseudomonas aeruginosa, and Klebsillapneumoniae phages with typical morphology, culture, and biochemical characteristics, four composites were prepared. Preparations, for the convenience of use and transportation, the compound preparations are concentrated preparations.

[0082] The first preparation contains

[0083] 1. The number of viable bacteria of Bacillus subtilis LY-35 in one milliliter of compound preparation is 1×10 9 .

[0084] 2. The phage titer of one milliliter compound preparation E.Coli, Salmonella D, enteriditi, Staphylococcus aureus, Streptococcus Pyogenes, Proteus mirabilis, Proteus Vulgaris, Pseudomonasaeruginosa, Klebsilla pneumoniae is 10 6 (ten-fold serial dilution method).

[0085] 3. Enzyme inhibition stabilizer: 0.5% EDTA (ethylenediaminetetraacetic a...

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Abstract

The invention relates to a bacilli, its compound preparation and the preparing method for the compound preparation. Its name is Bacillus subtilis LY-35, which is preserved in the general microbe center of Micro Germ conservation Management committee of China, and its conservation number is:CGMCC N0.1222. The preparation is a mixture of lambda phage of culture of bacilli and staphylococcus, streptococcus, colibacillus, bacillus proteus, pseudomonad, salmonella, pasteurella and klebsiella, can cure and prevent all kinds of illness caused by conditional pathogen,is a micro biological preparation which can be used by being mixed with antibiotics or replace antibiotics without any remainder and pollution, with good curative effect and safety.

Description

technical field [0001] The invention relates to a bacillus subtilis, a compound preparation prepared by using the strain, and a preparation method of the compound preparation. Background technique [0002] In recent years, with the development of my country's aquaculture industry and the improvement of intensification, bacterial diseases have become an obstacle restricting the further development of aquaculture industry. Especially suppurative inflammatory diseases caused by opportunistic pathogenic bacteria present in large quantities in the environment such as Staphylococcus, Streptococcus, Escherichia coli, Proteus, Pseudomonas, Salmonella, Shigella, Pasteurella and Klebsiella Widely popular. Due to the lack of scientific management and the abuse of antibiotics, not only the micro-ecological balance of the normal flora has been destroyed, but also a large number of drug-resistant strains have been produced, resulting in limited clinical treatment effects, resulting in re...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20
Inventor 阿尔申巴耶夫·穆罕默德古拉金·瓦列里巴伊古金娜·法尼丽娅扎伊努里·鲁斯特姆张晓静战军孙志强倪冬雁
Owner 烟台绿云生物工程研究院有限公司
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