Application of bacillus altitudinis Ba1449 and fungicide in prevention and treatment of plant fungal diseases

A highlander bacillus, ba1449 technology, applied in the fields of application, fungicides, plant growth regulators, etc., can solve the problems of undeveloped and undiscovered citrus anthracnose bacteria

Active Publication Date: 2021-12-10
GANNAN NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, no biocontrol bacteria that effectively inhibit citrus anthracnose have been developed, and bacteria that antagonize Alternaria citrus brown spot have not been found

Method used

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  • Application of bacillus altitudinis Ba1449 and fungicide in prevention and treatment of plant fungal diseases
  • Application of bacillus altitudinis Ba1449 and fungicide in prevention and treatment of plant fungal diseases
  • Application of bacillus altitudinis Ba1449 and fungicide in prevention and treatment of plant fungal diseases

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Citrus rhizosphere bacterial extracts:

[0030] Citrus materials tested: Citrus Citrus root samples were collected from Jiangxi (N25 ° 40'43 ", E115 ° 2'51");

[0031] Bacteria were citrus root extract dilution plate method: The soil sample containing root tissue of citrus freeze drying; a "shaking Root Law" shake off the roots (including fibrous) four weeks loose soil, sterile gloves by rubbing and shaking from the roots manually remove loose soil, about 1 ~ 2mm until the remaining soil attached to the root; soil sample containing about 1 ~ 2mm thickness placed in the root outer tube filled with PBS buffer, washed with sterile forceps root stirring Clear All soil surface, vortexing after 10min, taken root; the root of the wash buffer was centrifuged (4300rpm, 5min) separation, the supernatant was discarded, the precipitate portion rhizosphere microbial colonies; the plate on LB medium plate purification bacteria scribing method, Individual colonies were picked into LB brot...

Embodiment 2

[0033] 1, the test pathogens: citrus tenuis (A.alternata pv.citri) available from Ningbo Ming Zhou Biological Technology Co., Cat: BMZ026463; glue fungal spore anthrax (Colletotrichum gloeosporioides) available from Ningbo Ming Zhou Biological Technology Co., Ltd. , Item: BMZ105526.

[0034] 2, medium:

[0035] Formulation LB broth (g / L): tryptone, 10.0g / L, yeast extract 5.0g / L, NaCl 10.0g / L.

[0036] LB broth formulation methods (1L): 10g tryptone were sequentially added in 950ml double distilled water, 5g yeast extract and sodium chloride 10g. Adjusting the medium with 1mol / L NaOH solution to pH 7.0, the volume to 1L. 121 ℃, 20min autoclaving, 4 ℃ stored.

[0037] LB medium plate production methods: Add 15g agar in 1L LB liquid medium. When 121 ℃, autoclaved 20min, cooling to 50 ~ 60 ℃, pour plates prepared. When the medium temperature is not too high braking down, otherwise the cooling plate produces a large amount of condensed water.

[0038] PDA solid medium composi...

Embodiment 3

[0053] Identification of strain A for strain A of bacteriostatic (activated citrus chain cyclospocci and tapipotranscene) during the recorcinocture of the plurality of Siki RS II and ILLUMINA HISEQ 4000, respectively, three generations and second generations respectively All genome sequencing. The three generations of data is used for assembly of the whole genome, and the second-generation data has obtained highland spiral BA1449 genome sequences after assembled genome. The whole genome sequence of this strain has been submitted to the NCBI database (NiH.GOV)) Its Bioproject serial number is prjNA679141. Using GTDB-T K software, identification of strains based on all genome similarities, as shown figure 1 As shown, it is found that the bacteria having the maximum average nucleic acid determination ANI (Average Nucleotide Identity) is a highly subtilis, and the ANI value is 98.36%. Bacillus BA1449 and other near-edge species (Bacillus Bacillus, Bacillus, Bacillus Bacillus, Bacillus...

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Abstract

The invention relates to the field of biological prevention and treatment, in particular to an application of bacillus altitudinis Ba1449 and a fungicide in prevention and treatment of plant fungal diseases. The invention provides the application of the bacillus altitudinis Ba1449 in prevention and treatment of the plant fungal diseases, and the preservation number of the bacillus altitudinis Ba1449 is CGMCC No.22899. According to the application of the bacillus altitudinis Ba1449 and the fungicide in prevention and treatment of the plant fungal diseases, the bacillus altitudinis Ba1449 has high antagonistic ability on alternaria alternata brown spot bacteria and anthracnose bacteria at the same time, has no pollution to the environment, and has an important biocontrol application prospect.

Description

Technical field [0001] The present invention relates to the field of biological control, particularly applications involving upland Bacillus Ba1449 and agents for controlling plant fungus disease. Background technique [0002] It is the world's largest citrus fruit, but has been suffering from serious disease threat. Citrus Alternaria leaf spot and anthracnose are major citrus diseases, which are fungal pathogens, namely: Alternaria Alternaria orange pathogenic type (Alternaria alternatapathotype tangerine, also known as A.alternata pv.citri) and Colletotrichum gloeosporioides (Colletotrichum gloeosporioides). [0003] Citrus Alternaria leaf spot (Alternaria brown spot, ABS) is a disease in our country in recent years, some areas newly discovered major damage Citrus reticulata hybrids and some citrus, causing defoliation, dieback and fruit drop, not only affects yield, also affect sales of the fruit, causing serious damage, and the disease was gradually spreading and worsening. A...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N63/22A01P3/00C12R1/07
CPCC12N1/20A01N63/22
Inventor 高玉霞李智龙熊志伟欧阳毅龙云飞彭鑫源
Owner GANNAN NORMAL UNIV
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