43 results about "Proteus vulgaris" patented technology

He invention discloses a drug-resistant klebsiella pneumoniae phage RDP-KP-20004 and application thereof, wherein the preservation number of the phage is CGMCC No.21407, and the whole gene is sequenced and then is found to have no virulence gene and lysogenic gene, and the drug-resistant klebsiella pneumoniae phage RDP-KP-20004 provided by the invention has higher temperature tolerance and wider acid-base tolerance range, has strong cracking effect on pathogenic Klebsiella pneumoniae BK-20004 in a poultry breeding environment, and provides a phage source for the industrial production of phage for the prevention and treatment of the pathogenic Klebsiella pneumoniae BK-20004 in the poultry breeding environment; the klebsiella pneumoniae phage BK-20004 provided by the invention has very good advantages in treating diseases infected with klebsiella pneumoniae, and is relatively high in titer and good in prevention and treatment effect; and good application prospects are realized in the prevention and control of poultry pathogenic proteus vulgaris.

An isolongifolenone oxime ether derivative is prepared by the following steps of: taking isolongifolenone as the raw material, synthesizing isolongifolenone oxime through addition-elimination reaction, and performing alkylation reaction of the isolongifolenone oxime. Simultaneously, the isolongifolenone oxime ether derivative disclosed by the invention can inhibit bacteria, such as staphylococcus aureus, Escherichia coli, Klebsiella pneumoniae, bacillus proteus vulgaris and pseudomonas aeruginosa, and fungus, such as Aspergillus niger, exserohilum turcicum, corn sheath blight, alternaria musae Bovr.et Bat. and colletotrichum musae; and the isolongifolenone oxime ether derivative has the excellent insecticidal action for insect pests, such as aphid and rice planthopper.

The invention relates to a compound amoxicillin breast injectant prescription and a preparation method thereof. The compound amoxicillin breast injectant prescription comprises a suspending aid, an antioxidant and a solid dispersion carrier which are prepared through a special process to obtain white to off-white oily suspension. The compound amoxicillin breast injectant prescription disclosed by the invention has the advantages of simple preparation process, stable content, easy injection and the like. The composition disclosed by the invention is compounded from amoxicillin, clavulanate potassium and prednisolone; compared with other amoxicillin patent preparations, the composition has drug-tolerant bacteria for penicillins, and also has a high inhibition effect on the chromosome exzymes generated by klebsiella peneumoniae, bacillus proteus vulgaris and bacteroides fragilis; prednisolone has anti-inflammatory and antiallergic functions, and has a synergistic effect on bacterial infection and allergic inflammation.

An effective therapeutic agent for keloids and/or hypertrophic scars is provided. Specifically, an elastic fiber regenerating agent consisting of chondroitinase ABC derived from Proteus vulgaris and a therapeutic agent for keloids and/or hypertrophic scars comprising the regenerating agent as an active ingredient are provided.

The invention provides an application of a dibenzimidazolamine compound to preparation of an antimicrobial medicine. The dibenzimidazolamine compound has a structure shown as formula (I), and is simple in structure. The dibenzimidazolamine compound is relatively good in in-vitro antimicrobial activity, and performs quite high inhibitory activity of gram positive bacteria, such as staphylococcus aureus, methicillin-resistant staphylococcus aureus, bacillus subtilis, and micrococcus luteus, gram negative bacteria, such as escherichia coli, proteus vulgaris, pseudomonas aeruginosa, and salmonella typhi, and fungi, such as candida utilis, aspergillus flavus, saccharomyces cerevisiae, candida albicans, and candida. The dibenzimidazolamine compound is used for preparing an antibacterial and/or antifungal medicine.

The invention discloses a chondroitinase ABC producing recombinant yeast strain and application thereof and belongs to the technical field of bioengineering. According to the chondroitinase ABC producing recombinant yeast strain, chondroitinase ABC from Proteus vulgaris ATCC33420 is subjected to heterogeneous expression, and with pPIC9K carriers and through induction of methanol, secretory expression of the chondroitinase ABC in pichia pastoris GS115 can be achieve; by means of a designed chondroitinase ABC-enzymatic membrane reactor, efficient and continuous production of small-molecular chondroitin sulfate can be achieved, the chondroitinase ABC can be recycled and reutilized, and the production cost can be greatly reduced. By taking food-grade yeast as host strains, the chondroitinase ABC producing recombinant yeast strain can be safe and reliable, provide effective references for industrialized and green production of small-molecular chondroitin sulfate A, B and C, and meanwhile, save energy, reduce emission and achieve significant economic and social benefits.

The method is suitable for the technical field of bacteria detection, the invention discloses a triple TaqMan Real-Time PCR detection method for simultaneously distinguishing proteus mirabilis(P. mirabilis), proteus vulgaris (P.vulgaris) and proteus penesei (P.pennerei), and a kit. The primers and nucleotide sequences of proteusbacillus vulgaris are predicted through computer software, and three pairs (six) of primers capable of being used for simultaneously detecting three proteusbacillus vulgaris and nucleotide sequences of three probes are disclosed. The method has the characteristics of strong specificity, high sensitivity and accurate and reliable result. According to the method, the proteusbacillus vulgaris can be identified as a specific species while the proteusbacillus vulgaris isdetected, the defect that only proteus mirabilis can be distinguished in existing proteusbacillus vulgaris detection is overcome, the proteusbacillus vulgaris detection process is greatly simplified,the workload of operators is reduced, the detection period is shortened, and a powerful technical support can be provided for detecting the proteusbacillus vulgaris in the industries of medical treatment, medical detection and the like.

The invention discloses a chondroitin sulfate ABC lyase mutant and a secretory expression method thereof, and belongs to the technical field of bioengineering. According to the chondroitin sulfate ABClyase mutant and the secretory expression method thereof, site-directed mutagenesis is conducted on chondroitin sulfate ABC lyase from proteus vulgaris, secretory expression of the chondroitin sulfate ABC lyase is enhanced by fusing signal peptide, outer membrane protein knockout is conducted on a strain expressing the enzyme, and a culture strategy of the strain is further provided.

The invention discloses a single-chamber microbial electro-Fenton system and an application thereof. The single-chamber microbial electro-Fenton system comprises an anode, a cathode and an electrolytesolution; the anode and the cathode are arranged in a same single chamber; the anode comprises an anode base material formed by rolling multiple layers of carbon cloth and a plastic net, and the plastic net is arranged between the carbon cloth and used for separating the carbon cloth; mixed bacteria are loaded on the anode base material; the mixed bacteria are composed of rhodopseudomonas palustris, proteus vulgaris, bacillus toyonensis and alcanivorax pacificus; the cathode comprises a support made of a titanium mesh, and the support is filled with activated carbon powder loaded with graphene and cobalt; the electrolyte solution comprises sewage to be treated and added nutrient substances; and the mixed bacteria decompose organic matters in the sewage to be treated to generate electronsfor the cathode to use. The current density generated by degrading organic pollutants by the mixed bacteria loaded on the anode is high, the energy consumption required by the electro-Fenton reactionof the cathode can be met, and extra electric energy does not need to be consumed.

The invention provides a microbial antibacterial extract, which is derived from fruiting bodies of butyriboletus hainanensis and is an ethanol extract of the fruiting bodies of the butyriboletus hainanensis. The microbial antibacterial extract provided by the invention has a good antibacterial effect and has an inhibitory effect on staphylococcus aureus, staphylococcus epidermidis, bacillus subtilis, bacillus proteus vulgaris and the like, thus having a broad development space in the prevention and treatment of diseases caused by the staphylococcus aureus, the staphylococcus epidermidis, the bacillus subtilis, the bacillus proteus vulgaris and the like; therefore, the microbial antibacterial extract has a very good development and application prospect in the aspect of antibacterial drugs.

The invention discloses a Chinese-Western compound disinfectant used for laboratory disinfection and a preparation method thereof, belonging to the technical field of medicine; the ingredients of the disinfectant in the invention are composed of sassafras, amaranthus, purple back vegetable, yin xiangpi, oil Citrus leaves, garlic, salt bran, misty kudzu, sharp tail wind, sticky grass, wild chrysanthemum, willow root, scorpion tree lotus, chlorhexidine, povidone iodine and glutaraldehyde; the disinfectant of the present invention uses traditional Chinese and Western medicine The combined treatment method is more effective than the simple use of traditional Chinese medicine and western medicine. With the principle of sterilization, insecticide, disinfection and anti-inflammation, it can maximize the efficacy of Chinese medicine ingredients and western medicine, play a complementary and enhancing role, and improve the medicinal effect , has antimicrobial effect, can destroy the permeability barrier of cell wall, reduce the enzyme activity, denature the bacterial protein, and thus play a bactericidal effect. It is effective against Staphylococcus aureus, E. Bacteria, common Proteus, etc. have good sterilization and disinfection effects.

The invention relates to a gene chip and a test kit for detecting important pathogenic bacteria in aquatic products, wherein the gene chip comprises a solid matrix and an oligonucleotide probe composed of one or more serials selected from the following serials: (1) DNA serials selected from the spacers of 16S-23rS DNA of salmonella, Vibrio parahaemolyticus, Vibrio cholerae, Listeria monocytogenes, staphylococcus aureus, Suppurative streptococcus, Proteus mirabilis, Proteus Vulgaris and Paneth Proteus, and ipaH Virulence gene of Shigella; (2) complement DNA serials of the DNA serials selected from (1); (3) complement RNA serials of the DNA serials selected from (1) or (2). The chip and the test kit comprise simple and convenient operation, high veracity and strong repeatability in detecting the important pathogenic bacteria in aquatic products.

The present disclose relates to a method for preventing the decomposition of urine. The method includes adding a chemical to a liquid urine solution in a collection device to alter the pH of the resulting solution to a level that substantially prevents or significantly reduces the metabolism and reproduction of bacteria such as Escherichia coli, Proteus vulgaris, and Pseudomonas. The chemical composition may be an acid, such as a particulate, non-toxic, food grade acid that lowers pH to less than about 5.5, but more specifically to less than 4.0. The chemical composition may be an alkaline compound, such as potassium hydroxide, that raises pH to more than about 11.5. This method may be implemented so that urine is treated in the collection container for a patient concurrently with the urine collection. A kit implementing the method is disclosed.