94 results about "Prodigiosin" patented technology

The invention belongs to the field of biotechnological medicine and life and particularly relates to a high-prodigiosin-yield serratia marcescens strain Sm-128 and use thereof. The high-prodigiosin-yield serratia marcescens strain Sm-128 was collected in China Center for Type Culture Collection on November 13, 2010. The collection number of the strain is CCTCC No.M2010347. The serratia marcescens strain Sm-128 can be used for synthesizing prodigiosin. According to ultraviolet absorption spectrum and Fourier H nuclear magnetic resonance (H-NMR) spectrum analysis, the pigment produced by the Sm-128 strain is prodigiosin and the yield of the prodigiosin reaches 3 to 5 g / L. The pigment has high thermostability, oxidant resistance and reducer resistance and low photostability.

The invention discloses a method for preparing and purifying prodigiosin. According to the method, serratia marcescens is utilized to produce strains; a crude product of prodigiosin is obtained through fermentation culture of mother seeds of serratia marcescens and extraction and condensation of red metabolites; and the crude product of prodigiosin is purified by the methods of silica gel column chromatography and high performance liquid chromatographic separation and subjected to low temperature freeze drying under vacuum so as to obtain a high pure product of prodigiosin. The purity of the high pure product prepared in the invention is greater than 98%; the method for preparing the product is highly effective; organic solvents used in the method can be recovered for cyclic utilization through rotary evaporation.

The invention provides a marine bacterial strain screening from the marine bacteria which can produce prodigiosin and a method for fermentation production of prodigiosin by using the said strain. The said marine bacterial strain is Pseudomonas sp.105001 CCTCC M 205164. The red metabolite of the said strain - the prodigiosin compound of the invented product can be prepared by selecting Pseudomonas sp.105001 CCTCC M 205164 as production strain, preparing mother seeds, by fermentation culture and extracting red metabolite and purifying metabolite. The new marine bacterial strain provided by this invention is of stable heredity and quick reproduction, which can perform large scale fermentation production and the prodigiosin production using the said marine bacteria, opens a new approach for study and exploitation of marine microbial resource.

This invention discloses a method for preparing prodigiosin by separation utilizing big hole adsorption to adsorb prodigiosin directly in the fermented solution and realizing coupling of fermentation and separation to finish the process of concentration and initial impurities remove on the adsorption post. Then, according to the composition in the eluent, a suitable solvent system is applied to adsorb impurities instead of the products to get rather pure products.

The invention discloses Serratia marcescen capable of producing red pigment through fermentation and a method for producing red pigment by fermentation, belonging to the field of fermentation engineering. The method comprises the following steps: carrying out lixiviating, extraction and rotary evaporation so as to obtain a pigment concentrated liquid; separating and purifying through silicagel column chromatography to obtain a pigment component; carrying out UV-Vis (ultraviolet-visible) analysis so as to determine that the maximum absorption peaks are respectively located at the wavelengths of 530nm and 465nm under the condition that the pH value is 3.0 and 9.0; and measuring the accurate relative molecular mass of the pigment component through LC / MS (liquid chromatography-mass spectrometry) analysis so as to determine that the pigment is prodigiosin. In the method provided by the invention, an LB (load balance) liquid cultural medium is adopted for fermentation; and 50 mL of culture solution is contained in a 500mL triangular flask; sterilization is carried out for 15 minutes at the temperature of 115 DEG C; shaking culture is carried out at the temperature of 30 DEG C at the speed of 200r / min, and then centrifuging operation is carried out for 10 minutes at the speed of 8000r / min to remove impurities; and through extraction with acidic methanol, the prodigiosin Serratia marcescen strain pigment is produced and the yield is 150mg / L. Prodigiosin has important application prospect in the fields of tumor treatment, foods, cosmetics and the like.

The invention discloses Serratia marcescens LTH-2 the preservation number of which is CCTCCM2011209. The Serratia marcescens LTH-2 has the capabilities of dissolving Microcystis and generating prodigiosin. The Serratia marcescens LTH-2 can be used for producing algae metabolin prodigiosin and can eliminate Microcystis water bloom and the environment pollution caused by the water bloom of Microcystis.

The invention relates to a method for dyeing wool fabric by using bacterial dye of prodigiosin, which comprises the steps of: (1), dissolving the prodigiosin by using ethyl acetate or N,N-dimethyl formamide, and then adding the dissolved prodigiosin into water to make dyeing solution; and (2), dyeing wool fabric by using the dyeing solution, wherein use amount of the prodigiosin is 0.1-6% of massfraction of the wool fabric; dyeing bath ratio is 1:10 to 1:20; the pH value of the dyeing solution is adjusted to be 4.0-6.0; dyeing temperature is 80-90 DEG C; and dyeing time is 30-90 min. The dyeing method disclosed by the invention is simple; dyed wool fabric has excellent washing fastness and rubbing fastness, is colorful, excellent in antibiotic property, and at least 90% in bacteriostaticrate, and has wide application prospect.

The invention discloses a prodigiosin high-producing strain and a production method of the prodigiosin high-producing strain. The prodigiosin high-producing strain is named as serratiamarcescenszl3 and is stored in China Typical Culture Collection Center with a number of CCTCCNO: M201209 on April 4, 2012 in Wuhan University, Wuhan, China. The prodigiosin high-producing strain disclosed by the invention has the advantages of being fast in growth speed, stable in synthesis of prodigiosin, and high in expression index; after being cultured for 30 to 40 hours in 1 to 5% of a peanut powder culture medium on a 50L fermenting tank at 25 to 30 DEG C, the prodigiosin has a yield up to 6 to 8g / , and purity is more than 98%. With the adoption of a technology of fermenting in batch, fed-batch fermentation technical parameters, an optimized technology of agitating and digesting and a chromatography refine purifying technology provided by the invention, guidance is provided for industrial and scale production of the prodigiosin; and the prodigiosin can be applied to bio-pharmaceuticals and pharmaceutical chemical engineering fields.

The invention discloses a method for dyeing an acrylic fabric with bacterial dye prodigiosin. The method is characterized in that the amount of the dye used is 3 percent (o.w.f); the volume ratio of alcohol solvent to water is 1-2:2-3; the bath ratio is 1 to 40; the pH value is 4.0 to 6.0; the dyeing temperature is 90 to 95 DEG C; and the dyeing time is 50 minutes. The dyed acrylic fabric has high antibacterial property and a bacteriostasis rate of over 90 percent; and in addition, the dyed acrylic fabric has high washing fastness, high rubbing fastness, bright color and great application prospect.

The invention discloses a viscid Serratia marcescens with CGMCC.No.2593 and prodigiosin produced. The growth temperature of the strain is 28 DEG C to 38 DEG C, the pH value is 5 to 9, LB liquid medium is used for fermentation, 200mL of culture medium is filled in a 500mL triangular flask, sterilization is carried out under 1125 mutiplied by 121Pa for 20min, after being shaken for culture at temperature of 37 DEG C for 5 days, the culture medium is added with absolute ethyl alcohol and is shaken on a shaking bed for 20min, and then is placed statically for 10min, impurity removing is carried out with the centrifuge of 4000r / min for 10min, and through the extraction of absolute ethyl alcohol, prodigiosin Serratia marcescens pigment is produced with the output of 7.85g / L, the maximum absorption peak value m of the prodigiosin being 533.8nm and good heat stability. The prodigiosin obtained can be used as antineoplastic medicine, has important application prospect in the fields of foods, cosmetics and the like.

The invention relates to a method for dyeing acrylic fiber fabrics by utilizing bacterium dyestuff prodigiosin, which includes the steps of (1) dissolving the prodigiosin by using ethyl acetate or N, N-dimethylformamide and then adding dissolved prodigiosin into water to produce a dyeing solution, and (2) dyeing the acrylic fiber fabrics by utilizing the dyeing solution, wherein the dosage of theprodigiosin is 0.1-6% of that of acrylic fiber fabrics by mass, dyeing bath ratio is 1:5-20, potential of hydrogen (pH) of an adjusting dye solution is 4.0-6.0, dyeing temperature is 80-90 DEG C, anddyeing time is 30-90 minutes. Compared with a dyeding method of a mixed solution of carbinol or ethanol and the water, dye-uptake rate of the dyestuff is high, level-dyeing property is good, and the dyeing method is simple, easy to operate and low in device requirements. The dyed acrylic fiber fabrics produced by means the method have good washing fastness and abrasion-resisting fastness, bright color, good antibiotic property and wide application prospects, and antibacterial rate is more than 90%.

The invention provides a Serratia marcescens Xd-1 strain with an accession number of CGMCC No. 7734 and a synchronous extraction and fermentation production method for prodigiosin by using the strain. The method is characterized by comprising the following steps: preparing primary seed liquid of the strain; preparing secondary seed liquid of the strain; mixing a liquid fermentation medium with a volume of V and the secondary seed liquid with a volume of 1 to 8% of V in a container and carrying out fermentation for 6 to 18 h under the fermentation conditions of a rotating speed of 130 to 200 r / min, oxygen flux of 0.5 to 1.5 v / (v.m) and fermentation temperature of 25 to 35 DEG C; and adding an extractant with a volume of 8 to 20% of V into the container and continuing fermentation under the fermentation conditions, wherein total fermentation time is 32 to 64 h.

The invention provides a method for preparing prodigiosin fermentation liquor. The method takes grease derived from woody plant seeds as a fermentation carbon source; the fermentation liquor containing 15-30g / L of prodigiosin can be prepared by controlling the fermentation conditions. The method has the advantages that (1) the raw material is widely planted, low in price, convenient to obtain and high in sustainability, and the like, so that the phenomena of 'competing with human for oil and land', and the like can be avoided; (2) the woody plants adopted by the method can be planted in the environments such as barren mountains and slopes, desolate beach and swamp and front or back yards, saline-alkali soil which are not beneficial to the grain crop planting and production, so that the method has important significances in the aspects of protecting the ecological environment, improving the land economic benefit, protecting the cultivated land and the like; (3) the woody plant grease with low value is prepared into the medicine product prodigiosin with high additional value by fermentation, so that the method is wide in market prospect and has great development value.

The invention relates to the field of microorganisms, in particular to an application of bacillus prodigiosus ITBB B5-1 to production of prodigiosins. The preservation No. of the bacillus prodigiosusis CGMCC No.7416. The prodigiosins are produced by the bacterial strain, the yield can achieve 9.8-15.5g in each liter of culture mediums, and the yield is notably higher than that of a bactria strainwhich is reported. The restriction of being low in yield of the prodigiosins by the bacillus prodigiosus bacterial strain is overcome, and the bacillus prodigiosus ITBB B5-1 has wide application prospects in the medicine markets.

The invention belongs to the technical field of application of microbial secondary metabolites, and particularly relates to novel application of prodigiosin in resisting potato Y viruses. The prodigiosin is applied to preparation of medicines for preventing and controlling potato Y viruses. The medicines for preventing and controlling the potato Y virus are liquid or solid preparations containingprodigiosin. It is proved that a host factor Hsp70 is recruited to promote replication and infection of PVY so as to achieve virus infection and propagation in the host infection process of PVY, so the Hsp70 plays an important role in infection and replication of plant viruses. Based on the above research result, it is further determined that prodigiosin treatment can significantly improve the ubiquitination level of a plant host, promote ubiquitination of the HSP70 protein of the host, degrade or inhibit expression of the Hsp70 protein, activate the natural immune activity of the host plant,induce the plant host to generate systematic resistance and realize antiviral characteristics.

The invention discloses a prodigiosin producing strain, and a production method and applications thereof. The prodigiosin producing strain is Serratia marcescens BWL1001 assigned the accession numberCGMCC No. 18953. The method for producing prodigiosin includes the following steps: preparing a seed solution; transferring the seed solution to a fermentation medium taking soybean oil as a carbon source according to the ratio of 1%(v / v), and performing shake cultivation at a constant temperate of 28 DEG C for 24-28 h to obtain fermentation broth; and performing centrifugation on the fermentationbroth to collect an upper oil phase, adding 1-3 times volume of acid methanol with pH being 3, performing oscillation and uniform mixing, performing centrifugation after standing for 30 min, collecting an acid methanol phase, and obtaining a prodigiosin product after a solvent is removed. The prodigiosin shows good inhibition effects on the growth of microcystis aeruginosa; and the used strain isfast in growing speed, short in fermentation period, high in prodigiosin yield and low in production cost, and has important promotion effects on promoting the industrial application of the prodigiosin.

A serratia marcescens is named as Serratia marcescens subsp. PGPRB has a preservation number of CCTCC No:M2013617. A screening method of the Serratia marcescens subsp. PGPRB comprises the steps of strain separation and identification. The Serratia marcescens subsp. PGPRB can be used for preparing prodigiosin and can also be used for preparing lipopeptide serrawettin W2.

The invention provides a solid state fermentation production method for prodigiosin by using an inert carrier. The method is characterized by comprising the following steps: preparing a seed liquid of Serratia marcescens; and inoculating the seed liquid into a solid state fermentation medium according to a volume mass ratio of 4-15 ml / 100 g and carrying out culture for 24 to 48 h under the conditions of a temperature of 24 to 30 DEG C and relative humidity of 70 to 90% so as to obtain a solid state fermentation prodigiosin product; wherein the solid state fermentation medium is composed of the inert carrier, a carbon source, a nitrogen source, salt and water according to a mass ratio of 1: 0.5-3: 0.2-2: 0.01-0.05: 5-18.

The invention discloses a serratia marcescens engineering bacterium and application thereof in production of prodigiosin, and belongs to the technical field of biology. The invention provides a serratia marcescens engineering bacterium JNB5-1 delta cpxR capable of realizing high yield of prodigiosin. The serratia marcescens engineering bacterium JNB5-1 delta cpxR is obtained by knocking out a genefor coding response regulatory protein CpxR in serratia marcescens JNB5-1. After the serratia marcescens engineering bacterium JNB5-1 delta cpxR is inoculated into a fermentation culture medium and fermented for 96 hours, the yield of prodigiosin in fermentation liquor can be as high as 5.83 g / L and is improved by 41.9% compared with that of wild serratia marcescens JNB5-1.

The invention discloses a method of producing lipopolysaccharide having antibacterial activity by means of a Serratia marcescens NS-17 bacterial strain. The method includes the steps of activation of the bacterial strain, seed culture, fermenting culture, cell collection, cell wall disruption, and extraction of the lipopolysaccharide. The Serratia marcescens NS-17, which is screened and obtained from soil, can generate a pigment prodigiosin under culture at 30 DEG C, but not generates the prodigiosin under culture at 37 DEG C. In the method, culture temperature is strictly controlled and a simple culture medium is employed; after culture for 32 h, thalluses are collected through centrifugation; polysaccharides are extracted through a phenol-water method after repeated freezing-thawing cell wall disruption; alcohol precipitation is carried out and then the precipitate is washed with ethanol and acetone in gradient concentrations; and vacuum freeze drying is carried out to prepare the lipopolysaccharide having significant antibacterial activity. The prodigiosin polysaccharide has significant inhibition effect on various microorganisms, especially staphylococcus aureus.

The invention provides a preparation method for a carrier-free nano-drug based on natural pigment. The nano-drug is specifically prepared by using a hydrophobic drug of prodigiosin. A purpose of the preparation method is to form carrier-free nano-particles with tumor targeting through self-assembly in water based on the hydrophobic drug of the prodigiosin, so the anti-tumor effect is achieved, andit is more important that many clinical safety problems caused by a nano-carrier are solved.

The invention relates to a method for dyeing acrylic fiber fabrics by utilizing bacterium dyestuff prodigiosin, which includes the steps of (1) dissolving the prodigiosin by using ethyl acetate or N, N-dimethylformamide and then adding dissolved prodigiosin into water to produce a dyeing solution, and (2) dyeing the acrylic fiber fabrics by utilizing the dyeing solution, wherein the dosage of theprodigiosin is 0.1-6% of that of acrylic fiber fabrics by mass, dyeing bath ratio is 1:5-20, potential of hydrogen (pH) of an adjusting dye solution is 4.0-6.0, dyeing temperature is 80-90 DEG C, anddyeing time is 30-90 minutes. Compared with a dyeding method of a mixed solution of carbinol or ethanol and the water, dye-uptake rate of the dyestuff is high, level-dyeing property is good, and the dyeing method is simple, easy to operate and low in device requirements. The dyed acrylic fiber fabrics produced by means the method have good washing fastness and abrasion-resisting fastness, bright color, good antibiotic property and wide application prospects, and antibacterial rate is more than 90%.

The invention discloses gene-rcsB-deficient recombinant Serratia marcescens and application thereof and belongs to the technical field of biology. The invention provides a Serratia marcescens engineering strain JNB5-1[delta]rcsB capable of high yielding prodigiosin. The Serratia marcescens engineering strain JNB5-1[delta]rcsB is obtained through knocking out a gene encoding a transcriptional regulation factor RcsB from Serratia marcescens JNB5-1. By inoculating an LB liquid culture medium with the Serratia marcescens engineering strain JNB5-1[delta]rcsB and carrying out fermentation for 24h, the yield of the prodigiosin in fermentation broth can reach up to 116.48mg / L and is 2.28 times that of wild type Serratia marcescens JNB5-1.

The invention discloses serratia marcescens RZ 21-C6 and application thereof. The preservation number of the serratia marcescens RZ 21-C6 is CGMCCNo.12714. The mutant strain has the advantages that the utilization efficiency of a substrate, the genetic stability and the yield of prodigiosin are high, and the utilization effect of the mutant strain on xylose is particularly good. In a 5L fermentation tank, pesticide residue straw hydrolysate with xylose as a main component is low in price, a biomass carbon source is developed for production of the prodigiosin, the yield of the prodigiosin can reach 15-20 g / L during multi-batch fermentation, and the conversion rate of total reducing sugar directly used for prodigiosin synthesis is 20% or above. The method for producing the prodigiosin through the serratia marcescens at normal pressure and room temperature by means of plasma mutant strain and the utilization of the xylose and the pesticide residue straw hydrolysate by the serratia marcescens can promote achievement of prodigiosin industrialization, and the method can be used for the fields of biological medicines, ecological environmental management, agricultural biological controlling and the like.

The present invention discloses a CRISPR-Cas9 dual vector system suitable for genetic modification of serratia. The CRISPR-Cas9 dual vector system comprises a vector pCAS-p15A-sacB delta lambda-Red and a vector pTarget F-Gm. The CRISPR-Cas9 dual vector system makes up for limitations of a serratia gene editing system and provides a platform for verification of serratia gene functions and a research on a mechanism of production of secondary metabolites of the serratia strain. The constructed CRISPR-Cas9 dual vector system is used to conduct gene luxS knock out and replenishment experiments anda preliminary study is made on a co-regulatory mechanism of serratia YD25 secondary metabolite prodigiosin and Serrawettin W2. A positive regulation of a gene luxS on synthesis of the prodigiosin andSerrawettin W2 is determined.

The invention discloses a multi-purpose method of serratia marcescens strains, and belongs to the technical field of fermentation engineering. The components of a fermentation culture medium are adjusted, and metabolism products of a serratia marcescens SDSPY136 strain are respectively 2-Keto-D-gluconic acid and prodigiosins, so that multiple purposes of the serratia marcescens SDSPY136 strain arerealized. In the step (2), the content of the 2-Keto-D-gluconic acid in fermentation liquid is very high, and byproducts are very few, so that in a 2KGA fermentation culture medium, the specificity of the 2-Keto-D-gluconic acid produced by metabolism of the serratia marcescens SDSPY136 strain is very high, and the purification is convenient. In the step (3), the content of prodigiosin in the fermentation liquid is very high, and byproducts are very few, so that in the prodigiosin fermentation culture medium, the specificity of the prodigiosin produced by metabolism of the serratia marcescensSDSPY136 strain is very high, and the purification is convenient.

The invention discloses an oxymethyltransferase mutant and application thereof in production of prodigiosin, and belongs to the technical field of biology. The invention provides serratia marcescens engineering bacteria JNB5-1 / S53CPigF and JNB5-1 / G176C / M245C / S53CPigF capable of realizing high yield of prodigiosin. The serratia marcescens engineering bacteria S53CPigF and JNB5-1 / G176C / M245C / S53CPigF are used for expressing the S53CPigF and the G176C / M245C / S53CPigF respectively; and the serratia marcescens engineering bacteria JNB5-1 / S53CPigF and JNB5-1 / G176C / M245C / S53CPigF are respectively inoculated into a fermentation culture medium to be fermented for 96 hours, and the yield of prodigiosin in the fermentation liquor can be respectively up to 7.24 g / L and 7.02 g / L.

The present invention discloses a method for producing prodigiosin based on PNTs, belongs to the technical field of biology, and provides serratia marcescens engineered bacteria JNB5-1 / pigFPNT and JNB5-1 / pigNPNT capable of high production of prodigiosin. The serratia marcescens engineered bacteria JNB5-1 / pigFPNT and JNB5-1 / pigNPNT are obtained by respectively connecting nucleotide fragments shownin SEQ ID No.11 at 3' ends of prodigiosin synthesis gene clusters pigF and pigN of the serratia marcescens respectively. The serratia marcescens engineered bacteria JNB5-1 / pigFPNT and JNB5-1 / pigNPNT are respectively inoculated into a fermentation culture medium for fermentation for 96 h, so that the production of the prodigiosin in fermentation liquid is as high as 7.55 g / L and 8.37 g / L, respectively.

The invention discloses an Escherichia coli expression vector pBMcopA and an application thereof, the expression vector is composed of pBM16A-T, copA promoter and restriction endonuclease sites BamH Iand Nde I are added to the TA junction region of pBM16A-T. Also disclosed is an expression vector pBMcopA-pigC, which is constructed from pBMcopA, also includes the gene of interest, the gene of interest is prodigiosin condensase (pigC) gene. The invention also provides a construction method of the two expression vectors. The constructed expression vector can express the target gene in Escherichia coli containing copper ion ATP transporter and can be used as an optional plasmid for gene modification.