Multi-purpose method of serratia marcescens strains
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A technology of Serratia marcescens and Serratia marcescens, which is applied in the field of fermentation engineering to achieve the effects of easy purification and strong specificity
Inactive Publication Date: 2020-11-10
SHANDONG FOOD & FERMENT IND RES & DESIGN INST
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[0005] In addition, 2-Keto-D-gluconic acid (2-KGA) and Prodigiosins (PG) can be fermented by Serratia, but the existing Serratia Serratia can only metabolize one of these substances, and there is no report of Serratia strains that can produce both 2-keto-D-gluconic acid and prodigiosin.
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Embodiment 1
[0033] A multi-purpose method for Serratia marcescens strain, comprising steps:
[0034] 1) Preparation of strain seed solution
[0035] Serratia marcescens ( Serratia marcescens ) SDSPY-136 strain was transferred to the slant medium for activation. The components of the slant medium were: peptone 5 g / L, sodium chloride 5 g / L, beef extract 3 g / L, agar 20 g / L, pH Value 7.0.
[0036] 250mL Erlenmeyer flask is filled with 30mL liquid seed culture medium, the components of liquid seed culture medium are: peptone 5 g / L, sodium chloride 5 g / L, beef extract 3 g / L, pH value 7.0. Steam sterilization at 121℃ 20min, cool to room temperature, inoculate 2 rings of slant strains, place on a shaker with a rotation speed of 200rpm and a rotation radius of 40mm, and cultivate at 30°C for 12h, OD 600 It was 0.267, and it was fermented as a seed liquid.
[0037] 2) Preparation of 2-keto-D-gluconic acid
[0038] A 5L fermenter was used to prepare the fermentation medium according to the liq...
Embodiment 2
[0053] A multi-purpose method for Serratia marcescens strain, comprising steps:
[0054] 1) Preparation of strain seed solution
[0055] The Serratia marcescens SDSPY-136 strain was transferred to the slant medium for activation. The components of the slant medium were: peptone 5 g / L, sodium chloride 5 g / L, beef extract 3 g / L, agar 20 g / L, pH 7.0.
[0056] 250mL Erlenmeyer flask is filled with 30mL liquid seed culture medium, the components of liquid seed culture medium are: peptone 5 g / L, sodium chloride 5 g / L, beef extract 3 g / L, pH value 7.0. Steam sterilization at 121℃ 20min, cool to room temperature, inoculate 2 rings of slant strains, place on a shaker with a rotation speed of 200rpm and a rotation radius of 40mm, and cultivate at 30°C for 12h, OD 600 It was 0.267, and it was fermented as a seed liquid.
[0057] 2) Preparation of 2-keto-D-gluconic acid
[0058] 250mL Erlenmeyer flask is equipped with 30mL 2-KGA fermentation medium, the components of 2-KGA fermentatio...
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Abstract
The invention discloses a multi-purpose method of serratia marcescens strains, and belongs to the technical field of fermentation engineering. The components of a fermentation culture medium are adjusted, and metabolism products of a serratia marcescens SDSPY136 strain are respectively 2-Keto-D-gluconic acid and prodigiosins, so that multiple purposes of the serratia marcescens SDSPY136 strain arerealized. In the step (2), the content of the 2-Keto-D-gluconic acid in fermentation liquid is very high, and byproducts are very few, so that in a 2KGA fermentation culture medium, the specificity of the 2-Keto-D-gluconic acid produced by metabolism of the serratia marcescens SDSPY136 strain is very high, and the purification is convenient. In the step (3), the content of prodigiosin in the fermentation liquid is very high, and byproducts are very few, so that in the prodigiosin fermentation culture medium, the specificity of the prodigiosin produced by metabolism of the serratia marcescensSDSPY136 strain is very high, and the purification is convenient.
Description
technical field [0001] The invention relates to the technical field of fermentation engineering, in particular to a multi-purpose method for one Serratia marcescens strain. Background technique [0002] 2-Keto-D-gluconic acid (2-KGA) is an important organic acid produced by bacterial fermentation, and is synthesized from food antioxidant D-isoascorbic acid and its salts key intermediates. 2-KGA can also be used as cement plasticizer, photo developer, detergent, calcium-rich additive for feed and herbicide, etc. It is an important raw material for the biosynthesis of aldose, ribulose and D-arabinose. The main production methods of 2-KGA are enzymatic catalysis, chemical synthesis and fermentation. Due to the advantages of low production cost, less by-products, and environmental protection, the microbial fermentation method is favored by researchers at home and abroad. At present, industrial production strains usually use Pseudomonas fluorescens, Jiang Mingzhu and others ha...
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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12P7/60C12P17/16C12R1/43
CPCC12N1/20C12P7/60C12P17/165
Inventor 徐慧田延军刘建军王珊珊周思多韩延雷李瑞国黄艳红
Owner SHANDONG FOOD & FERMENT IND RES & DESIGN INST
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