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Method for preparing prodigiosin fermentation liquor

A technology for producing prodigiosin and prodigiosin, which is applied in the field of fermented liquid for producing prodigiosin, can solve problems such as backward technology, low output, and low oil utilization efficiency, and achieve broad market prospects and ecological protection The effect of improving the environment and economic benefits

Active Publication Date: 2014-07-16
黄山市开发投资集团有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, in the world, soybean oil, peanut oil and sesame oil are mainly used as substrates for fermentation, or the seeds of gramineous oil crops are crushed and then fermented. The utilization efficiency of oil is low and the cost is high.
Due to the difference in substrate properties, the original technology is not suitable for woody oil fermentation to produce prodigiosin; the development and utilization of woody oil plants in my country is still in the early stage of development, and the current existing problems are scattered planting, backward management and low yield of woody oil plants The main reason is that the development of woody vegetable oils has not received enough attention and support, and the technical level of development is backward. Improving the application level and added value of woody vegetable oils has important economic value and great social significance
So far, there is no report on applying the fermented liquid of prodigiosin to the woody vegetable oil of the present invention

Method used

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  • Method for preparing prodigiosin fermentation liquor

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Preparation of woody vegetable oil: Take the mature seeds of Pistacia chinensis, dry them until the moisture content is 5%, and grind them mechanically to 15 mesh; During the process of pressing, crude oil is obtained; the crude oil is hydrated and degummed, acid neutralized, deodorized, and decolorized to obtain the finished pistachio oil. The fatty acids contained in Pistacia oil mainly include palmitic acid, oleic acid, linoleic acid, palmitoleic acid, stearic acid, arachidonic acid, and linolenic acid, of which the sum of oleic acid, linoleic acid, and palmitic acid accounts for About 95% of the total. The oil content of Pistacia chinensis is 56.5%, the oil yield of seeds is 20%-30%, and the oil content of husk is 3.28%. It is a non-drying oil with a light yellow-green color and a bitter taste.

[0023] (1) Preparation of seed medium: distilled water as solvent, ingredients and mass ratio: 1% woody vegetable oil, 1.5% yeast peptone, 1.5% ammonium sulfate, 0.1% pota...

Embodiment 2

[0028] Preparation of woody vegetable oil: Take the mature seeds of the glabra tree, dry them to a moisture content of 8%, and grind them mechanically to 10 meshes; Press at ℃ to obtain crude oil; the crude oil is hydrated and degummed, neutralized acid, deodorized, and decolorized to obtain eucalyptus seed oil. The fatty acid composition of eucalyptus glabra seed oil is mainly linoleic acid and Oleic acid, the described eucalyptus is a kind of woody oil plant, and the pulp and seed kernel all contain more oil.

[0029] (1) Preparation of seed medium: use distilled water as solvent; ingredients and their mass ratios are: woody vegetable oil 2%, yeast peptone 3%, ammonium sulfate 1%, potassium dihydrogen phosphate 0.1%, sodium nitrate 0.2%, Magnesium sulfate heptahydrate 0.05%; pH value is 7.5; prepared to obtain seed culture medium.

[0030] (2) Preparation of fermentation medium: use distilled water as solvent; ingredients and their mass ratios are: woody vegetable oil 12%, ...

Embodiment 3

[0034] Preparation of woody vegetable oil: Take the mature seeds of tung tree, dry them until the water content is 5%, and grind them mechanically to 10 meshes; When the oil is pressed, crude oil is obtained; the crude oil is hydrated and degummed, acid neutralized, deodorized, and decolorized to obtain tung oil. The refractive index of tung oil is 1.5206, the acidification value is 5.1 mg / g, the iodization value is 165 mg / g, the saponification value is 190 mg / g, the transparency is transparent, the color value is 0.1, the smell has no peculiar smell, and the density is 0.9376 g / mL, the thermal polymerization time is 446 seconds, and contains β-tung acid. The fruit of the tung tree contains about 10 kernels, and the oil content of the kernels is about 62.89%.

[0035] (1) Preparation of seed medium: use distilled water as solvent; ingredients and their mass ratios are: 1% woody vegetable oil, 1% yeast peptone, 1% ammonium sulfate, 0.15% potassium dihydrogen phosphate, 0.2% sod...

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Abstract

The invention provides a method for preparing prodigiosin fermentation liquor. The method takes grease derived from woody plant seeds as a fermentation carbon source; the fermentation liquor containing 15-30g / L of prodigiosin can be prepared by controlling the fermentation conditions. The method has the advantages that (1) the raw material is widely planted, low in price, convenient to obtain and high in sustainability, and the like, so that the phenomena of 'competing with human for oil and land', and the like can be avoided; (2) the woody plants adopted by the method can be planted in the environments such as barren mountains and slopes, desolate beach and swamp and front or back yards, saline-alkali soil which are not beneficial to the grain crop planting and production, so that the method has important significances in the aspects of protecting the ecological environment, improving the land economic benefit, protecting the cultivated land and the like; (3) the woody plant grease with low value is prepared into the medicine product prodigiosin with high additional value by fermentation, so that the method is wide in market prospect and has great development value.

Description

technical field [0001] The invention belongs to the technical field of fermentation, and in particular relates to a method for producing a fermentation liquid of prodigiosin. Background technique [0002] Prodigiosin is a small red pigment molecule with a tripyrrole ring structure, which has been proven to have various biological activities such as immunosuppression, anti-cancer, anti-tumor, anti-bacteria, anti-fungal, anti-mold and anti-malarial. Research by the National Cancer Institute of the United States has shown that the average half inhibitory concentration (IC) of prodigiosin 50 ) is 2.1 μmol / L, prodigiosin has strong resistance to 57 different human cancer cells, and also has resistance to liver cancer metastasis in vitro and human primary cancer cells and other human cancer cells. Malignant cell lines were not overtly toxic. Prodigiosin has become a very potential anti-cancer substance because of its high pertinence to cancer tissue and low toxicity to normal ce...

Claims

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Application Information

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IPC IPC(8): C12P17/16C12R1/43
Inventor 杨培周姜绍通程晓蕾张丹峰郑志罗水忠潘丽军苏文娟
Owner 黄山市开发投资集团有限公司
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