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Preparation method for carrier-free nano-drug based on natural pigment and application

A technology of natural pigments and nano-drugs, applied in the field of preparation of carrier-free nano-drugs, can solve the problems of poor water solubility of chemotherapeutic drugs, complex preparation of nano-carriers, and toxicity of metabolism and excretion, and achieves good anti-tumor effect and water-solubility effect. Good, less toxic and side effects

Active Publication Date: 2018-12-21
FUZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the pharmaceutical activity of prodigiosin has been widely recognized, its strong hydrophobicity and almost insoluble in water lead to low bioavailability in vivo, which seriously affects its wide clinical use.
[0004] In order to solve the limiting factors such as poor water solubility, no targeting, and low bioavailability of chemotherapy drugs, nanocarriers such as liposomes, micelles, mesoporous silica, and gold nanoparticles are used to improve cancer treatment.
However, the preparation of nanocarriers is extremely complex, and the degradation, metabolism, and excretion of nanocarriers can cause significant toxicity issues

Method used

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  • Preparation method for carrier-free nano-drug based on natural pigment and application
  • Preparation method for carrier-free nano-drug based on natural pigment and application
  • Preparation method for carrier-free nano-drug based on natural pigment and application

Examples

Experimental program
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Effect test

Embodiment 1

[0038] Example 1 Preparation method of prodigiosin nanometers Accurately weigh 0.00324g of PG powder, dissolve in 1ml of methanol, ultrasonically dissolve, and configure a 10 mM solution; take different volumes of the above solutions and dissolve them in 100μL of methanol, and dissolve them in 1ml of methanol. Add dropwise to 1 mL of secondary water (double distilled water) (note: vortex during the dropping process), at this time, the concentration of PG in the solution is 10μM-640μM, after ultrasonication for 10min, dry methanol , that is, PG nanometer; and use the precipitation method to determine the drug loading capacity of PG molecules in the nanomedicine (Drug loading capacity). Table 1 shows the average particle size, PDI, potential and drug loading of different concentrations of PG prepared in this example.

[0039] Table 1 The average particle size, PDI, potential and drug loading of different concentrations of PG nanoparticles

[0040]

Embodiment 2

[0042] Accurately weigh 0.00324g of PG powder, dissolve it in 1mL of methanol, ultrasonically dissolve, and configure a 10 mM solution; take different volumes of methanol solution, and add dropwise to the solution containing 1 mL of secondary water (double distilled water) during the stirring process. ) (note: vortex during the dropping process), the concentration of PG in the solution is 160μM at this time, after ultrasonication for 10min, dry the methanol to get PG nanometer;

[0043] The PG nanoparticles prepared in this embodiment have a diameter of 155.6 and a potential of 50.2mV such as figure 1 and figure 2 shown.

Embodiment 3

[0045] The PG nano-medicine prepared in Example 2 and the PG methanol solution of the same concentration were detected by ultraviolet-visible spectrophotometer, and the results showed that the nano-anticancer drug prepared by the present invention still had PG characteristic absorption peaks, such as image 3 As shown, it is proved that PG self-assembly of the present invention forms PG NPs anticancer nanomedicine.

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Abstract

The invention provides a preparation method for a carrier-free nano-drug based on natural pigment. The nano-drug is specifically prepared by using a hydrophobic drug of prodigiosin. A purpose of the preparation method is to form carrier-free nano-particles with tumor targeting through self-assembly in water based on the hydrophobic drug of the prodigiosin, so the anti-tumor effect is achieved, andit is more important that many clinical safety problems caused by a nano-carrier are solved.

Description

technical field [0001] The invention relates to the technical field of biomedicine, in particular to a preparation method and application of a carrier-free nano-medicine based on natural pigments. Background technique [0002] Cancer (Cancer), also known as malignant tumors, is the main cause of human death in both developed and developing countries. Factors such as economic development and economic development will lead to a continuous increase in the number of cancer patients. Current tumor treatment methods mainly include: chemotherapy, surgical resection, radiotherapy, photodynamic therapy, gene therapy and so on. [0003] Prodigiosin (PG) is a typical alkaloid secondary metabolite produced by bacteria, actinomycetes, etc., and is a general term for a class of natural red pigments with a three-pyrrole ring structure. Prodigiosin has diverse biological activities, including antibacterial, antifungal, antimalarial, anticytotoxic, anticancer, antitumor metastasis, immunos...

Claims

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Application Information

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IPC IPC(8): A61K31/4025A61P35/00
CPCA61K31/4025A61P35/00
Inventor 邵敬伟赵瑞瑞张冰晨
Owner FUZHOU UNIV
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