Paris total saponins extract with tumor metastasis resisting action and pharmaceutical formulation thereof

A technology of anti-tumor metastasis and pharmaceutical preparations, which is applied in the field of total saponin extracts of Papaya and its pharmaceutical preparations. It can solve the problems that the anti-tumor metastasis of saponin extracts has not been found, and achieve the effect of small toxic and side effects

Inactive Publication Date: 2008-07-09
TIANJIN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Modern pharmacological studies have shown that the alcohol extract and water extract of Chonglou have inhibitory effects on various tumor cells in vitro, and have obvious inhibitory effects on the growth of animal transplanted tumors S180, S37, EAC, ARS, L759, etc. But so far, it has not been found that the total saponins extract of Chonglou has anti-metastatic effect and its application in anti-metastatic drugs has been reported.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0014] One, the preparation of total saponins extract

[0015] After passing through a 5-mesh sieve, 500 g of the coarse powder of Dianzhonglou produced in Yunnan was soaked with 4 times the volume ratio of 70% and 85% ethanol for 1 hour, then heated and refluxed at a temperature of 85°C for 3 times, each time for 2 hours, and combined The extract is concentrated to obtain an extract; add the above extract to 20% ethanol with a volume ratio of 100 times and disperse evenly, centrifuge, dissolve after precipitation, and centrifuge again, repeat twice, and combine the supernatant; combine the above The supernatant is passed through a macroporous adsorption resin chromatography column, the amount of dry resin used is calculated according to the weight ratio of the extract to 5:1, the column flow rate is 1-3BV / h, and the eluent is discarded; use 60%-80% ethanol For elution, the flow rate is 2-5BV / h, and the ethanol eluate is collected, concentrated under reduced pressure and vacuu...

Embodiment 1

[0038] Capsules: (by weight percentage)

[0039] Total Saponins of Parisia 66%

[0040] Cornstarch 5%

[0041] Dextrin 10%

[0042] Microcrystalline Cellulose 10%

[0043] Calcium Monohydrogen Phosphate 3%

[0044] Micronized silica gel 6%

[0045] Add appropriate amount of 85% ethanol to the above auxiliary materials to make a soft material, pass through a 20-mesh sieve to granulate, dry at 40°C for 1 hour, pass through a 20-mesh sieve for granulation, pour the obtained granules into capsules, and make a finished product.

Embodiment 2

[0047] Injection: (by weight percentage)

[0048] Total Saponins of Papaya 98.1%

[0049] Sodium chloride 0.9%

[0050] Tween-80 1%

[0051] Take water for injection and boil it, put in total saponins of Parisia, stir well, boil for 3-7 minutes, cool to room temperature, filter, add sodium chloride, foot plate, heat to 65°C, add Tween-80, stir well, filter, Filter through a 0.22μm microporous membrane, dispense, steam sterilize for 1 hour, and fill.

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Abstract

The present invention provides a paris total saponin extract for resisting tumor metastasis and a pharmaceutical preparation thereof. The pharmaceutical preparation is composed of paris total saponin extract as the active component and medicinal auxiliary materials; wherein, the weight percentage of paris total saponin extract in the total weight of the pharmaceutical preparation is 60 to 98 percent. The paris total saponin extract for resisting tumor metastasis and the pharmaceutical preparation provided by the present invention have good inhabitation on the metastasis of animal transplanted tumor cells, demonstrated in (1) significant anti-metastasis function for inbred mice and nude mice metastasis model of vaccinated mouse lung adenocarcinoma, human lung cancer, human gastric cancer, human intestinal cancer, human liver cancer and other tumor strains; (2) significant effect on relative albumen metastasis; (3) significant effect on relative gene metastasis and no obvious toxicity on major viscera. In addition, the main active component of the pharmaceutical preparation is a Chinese medicine extract, so compared with chemical drugs, the present invention has the advantage of small toxicity.

Description

technical field [0001] The invention belongs to the field of pharmacy, and in particular relates to an extract of total saponins of polyphylla with anti-tumor metastasis and a pharmaceutical preparation thereof. Background technique [0002] Malignant tumors are common and frequently-occurring diseases that seriously threaten human health. According to the report of the World Health Organization, among the more than 5 billion people in the world, about 9 million people are newly diagnosed with cancer every year, and 7 million people die due to cancer, accounting for Nearly 20% of the total fatality rate, so the development of anticancer drugs has become a major topic of new drug research in this century. At present, the drug treatment of cancer at home and abroad, especially the molecular targeted therapy, is constantly improving the treatment effect of cancer, but at the same time, it should be noted that many chemotherapeutic drugs may cause a variety of short-term and lon...

Claims

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Application Information

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IPC IPC(8): A61K36/896A61K31/7048A61K9/48A61K9/08A61K9/20A61K9/16A61K9/14A61K9/10A61K9/02A61P35/00A61K125/00
Inventor 高文远颜璐璐满淑丽
Owner TIANJIN UNIV
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