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Solid state fermentation production method for prodigiosin

A technology for solid-state fermentation and prodigiosin, which is applied in the field of fermentation industry, can solve the problems of inability to meet industrialization needs, low fermentation level, and large difference in yield, and achieves low production process and equipment requirements, simple extraction process, and reduced emissions. amount of effect

Active Publication Date: 2014-03-12
UNIV OF SHANGHAI FOR SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, at present, the fermentation of prodigiosin and its analogs is mainly based on liquid fermentation, and the fermentation level is low. It is still in the stage of laboratory shake flasks, and the yield of different researchers varies greatly. Generally speaking, it cannot meet the needs of industrialization.
For example, the publication numbers are CN102002469A (name of invention: prodigiosin-producing strain and its method), CN102277323A (name of invention: Serratia marcescens Sm-128 strain with high prodigiosin production and its application ), CN101392227B (invention name: a Serratia marcescens and the prodigiosin produced by it) and other patent documents disclose a series of methods for the production of prodigiosin by Serratia marcescens fermentation. The methods are all liquid culture, and the prodigiosin production is between 4-10g / L, compared with foreign fermentation levels, there is still a big gap
However, the methods related to the production capacity of prodigiosin by solid-state fermentation have not been reported. Therefore, in order to improve the production of prodigiosin and meet the needs of industrial production and application, it is very necessary to develop a good solid-state fermentation method

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] (1) Serratia marcescens was inoculated on LB slant medium at a culture temperature of 30°C for 24 hours, and then inoculated into liquid seed medium. The liquid seed medium is (g / L): tryptone 10, yeast powder 5, NaCl10, K 2 HPO 4 0.1, MgSO 4 0.1. The cultivation conditions of the liquid seeds are as follows: cultivation temperature 30° C., shaker rotation speed 200 r / min, pH 6.5, cultivation time 16 hours, to obtain a first-grade seed liquid.

[0018] According to the 10% inoculum amount, put the primary seed liquid into the 10L seed tank, and add 7L seed medium to the 10L seed tank. The cultivation temperature was 30°C, the rotation speed of the shaker was 200r / min, the ventilation rate was 1.5v / (v.m), the pH was 6.5, and the cultivation time was 12h to obtain the secondary seed liquid.

[0019] (2) Put an appropriate amount of bagasse into the culture container, and the medium composition is (g / g bagasse): glycerol 0.5, peptone 0.2, MgSO4·7H2O0.01, K 2 HPO 4 0.0...

Embodiment 2

[0022] (1) Serratia marcescens was inoculated on LB slant medium at a culture temperature of 30°C for 24 hours, and then inoculated into liquid seed medium. The liquid seed medium is (g / L): tryptone 10, yeast powder 5, NaCl10, K 2 HPO 4 0.1, MgSO 4 0.1. The cultivation conditions of the liquid seeds are as follows: cultivation temperature 30° C., shaker rotation speed 200 r / min, pH 6.5, cultivation time 16 hours, to obtain a first-grade seed liquid.

[0023] According to the 10% inoculum amount, put the primary seed liquid into the 10L seed tank, and add 7L seed medium to the 10L seed tank. The cultivation temperature was 30°C, the rotation speed of the shaker was 200r / min, the ventilation rate was 1.5v / (v.m), the pH was 6.5, and the cultivation time was 12h to obtain the secondary seed liquid.

[0024] (2) Put an appropriate amount of bagasse into the culture container, and the medium composition is (g / g bagasse): glycerol 1.2, peptone 0.8, MgSO4·7H2O0.03, K 2 HPO 4 0.0...

Embodiment 3

[0027] (1) Serratia marcescens was inoculated on LB slant medium at a culture temperature of 30°C for 24 hours, and then inoculated into liquid seed medium. The liquid seed medium is (g / L): tryptone 10, yeast powder 5, NaCl10, K 2 HPO 4 0.1, MgSO 4 0.1. The cultivation conditions of the liquid seeds are as follows: cultivation temperature 30° C., shaker rotation speed 200 r / min, pH 6.5, cultivation time 16 hours, to obtain a first-grade seed liquid.

[0028] According to the 10% inoculum amount, put the primary seed liquid into the 10L seed tank, and add 7L seed medium to the 10L seed tank. The cultivation temperature was 30°C, the rotation speed of the shaker was 200r / min, the ventilation rate was 1.5v / (v.m), the pH was 6.5, and the cultivation time was 12h to obtain the secondary seed liquid.

[0029] (2) Put an appropriate amount of bagasse into the culture container, and the composition of the medium is (g / g bagasse): glycerol 2.5, peptone 1.8, MgSO4 7H2O0.05, K 2 HPO...

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Abstract

The invention provides a solid state fermentation production method for prodigiosin by using an inert carrier. The method is characterized by comprising the following steps: preparing a seed liquid of Serratia marcescens; and inoculating the seed liquid into a solid state fermentation medium according to a volume mass ratio of 4-15 ml / 100 g and carrying out culture for 24 to 48 h under the conditions of a temperature of 24 to 30 DEG C and relative humidity of 70 to 90% so as to obtain a solid state fermentation prodigiosin product; wherein the solid state fermentation medium is composed of the inert carrier, a carbon source, a nitrogen source, salt and water according to a mass ratio of 1: 0.5-3: 0.2-2: 0.01-0.05: 5-18.

Description

technical field [0001] The invention relates to a fermentation method for producing prodigiosin, in particular to a solid-state culture fermentation method for producing prodigiosin, belonging to the field of fermentation industry. Background technique [0002] Prodigiosin is a general term for a family of natural red pigments, usually having a methoxypyrrole skeleton structure composed of three pyrrole rings. Natural prodigiosin compounds can be divided into four structural types. The first is a linear structure, such as prodigiosin and undecyl prodigiosin; the other three are cyclic derivatives of prodigiosin. The prodigiosin-producing strains in nature are mainly Serratia marcecens, in addition to some actinomycetes (eg: Streptomyces coelicolor), and marine bacteria (eg: Hahella chejuensis, Pseudoalteromonas denitrificans). [0003] In recent years, it has attracted more and more attention because of its good effects on immunosuppression and anticancer. At the same time...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P17/16C12R1/43
Inventor 艾连中夏永军徐斐侯建平陈卫
Owner UNIV OF SHANGHAI FOR SCI & TECH
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