30 results about "P. mirabilis" patented technology

A method and a composition for treatment of pulmonary bacterial infections caused by gram-negative bacteria suitable for treatment of infection caused by Escherichia coli, Klebsiella pneumoniae, Klebsiella oxytoca, Pseudomonas aeruginosa, Haemophilus influenzae, Proteus mirabilis, Enterobacter species, Serratia marcescens as well as those caused by Burkholderia cepacia, Stenotrophomonas maltophilia, Alcaligenes xylosoxidans, and multidrug resistant Pseudomonas aeruginosa, using a concentrated formulation of aztreonam, or a pharmaceutically acceptable salt thereof, delivered as an aerosol or dry powder formulation.

The invention discloses a compound bacteria culture composition, which includes proteus morganii culture, proteus mirabilis culture, filamentous Streptomyces culture, bacillus thuringiensis culture and candida mycoderma bacteria culture. The compound bacteria culture composition is prepared by mixed-culturing proteus morganii culture, proteus mirabilis culture, filamentous Streptomyces culture, bacillus thuringiensis culture and candida mycoderma bacteria culture according to part by weight; under the condition of tolerating toxicity of heavy metals, the composition releases antibiotic and canplay safe and effective purifying effect to sewage commonly polluted by pathogenic bacteria and heavy metals.

The invention provides a multiplex PCR primer set for avian pathogenic Escherichia coli, Pasteurella multocida, Proteus mirabilis, Pseudomonas aeruginosa, Salmonella and Staphylococcus aureus, and multiplex PCR comprising the primer set A detection kit and a multiplex PCR detection method using the primer set, the primer set includes: a primer pair PhoA‑F and PhoA‑R for specific amplification of the phoA gene of avian Escherichia coli; a specific amplification of the Pasteurella multocida KMT1 gene The primer pair KMT‑F and KMT‑R; the primer pair AtpD‑F and AtpD‑R of the specific amplification Proteus mirabilis AtpD gene; the primer pair PETA‑F and PETA‑R of the specificity expansion Pseudomonas aeruginosa PETA gene; Primer pair InvA‑F and InvA‑R for specific amplification of Salmonella InvA gene; primer pair NuC‑F and NuC‑R for specific amplification of Staphylococcus aureus nuc gene.

The invention discloses a specific nucleic acid marking sequence of proteus mirabilis, a PCR detecting method and the application. The aim of the invention is to provide the specific nucleic acid marking sequence of the proteus mirabilis, a qualitative and quantitative PCR detecting method of the proteus mirabilis and the application in preparing qualitative and quantitative PCR detecting kits ofthe proteus mirabilis. The specific nucleic acid marking sequence of the proteus mirabilis is one of following nucleic acid sequences: firstly, a DNA sequence of SEQ ID NO: 1 in the table, secondly, a restricted DNA sequence of the SEQ ID NO: 1 in the table, which has the homology more than 90% and is the specific nucleic acid sequence of the proteus mirabilis, and thirdly, a nucleic acid sequence which can cross-breed with the restricted DNA sequence of the SEQ ID NO: 1 in the table under the high-stringent condition. The invention can be used in qualitative and quantitative molecular detection of the proteus mirabilis (including the detecting methods of ordinary PCR, quantitative PCR, chips, hybrid and the like) and has broad application prospect.

The invention discloses rana margaratae antimicrobial peptide odorranain-H-OM1, which is protein having an amino acid sequence as shown by SEQIDNO:1 in a sequence table, or protein obtained by substituting, deleting or adding more than one amino-acid residue to an amino-acid residue sequence as shown by SEQIDNO:2, having the same activity with the amino-acid residue sequence as shown by the SEQIDNO:2 and deriving from the SEQIDNO:2. The coding gene of the rana margaratae antimicrobial peptide odorranain-H-OM1 is obtained by cloning the rana margaratae antimicrobial peptide odorranain-H-OM1, and the odorranain-H-OM1 is synthesized by using a chemical synthesis method. The antimicrobial peptide is small in molecular weight, and has a strong killing effect on balcillus proteus mirabilis, enterococcus faecium, ampicillin-resistant escherichia coli and candida glabrata. Besides, the antimicrobial peptide is low in hemolytic activity.

The method is suitable for the technical field of bacteria detection, the invention discloses a triple TaqMan Real-Time PCR detection method for simultaneously distinguishing proteus mirabilis(P. mirabilis), proteus vulgaris (P.vulgaris) and proteus penesei (P.pennerei), and a kit. The primers and nucleotide sequences of proteusbacillus vulgaris are predicted through computer software, and three pairs (six) of primers capable of being used for simultaneously detecting three proteusbacillus vulgaris and nucleotide sequences of three probes are disclosed. The method has the characteristics of strong specificity, high sensitivity and accurate and reliable result. According to the method, the proteusbacillus vulgaris can be identified as a specific species while the proteusbacillus vulgaris isdetected, the defect that only proteus mirabilis can be distinguished in existing proteusbacillus vulgaris detection is overcome, the proteusbacillus vulgaris detection process is greatly simplified,the workload of operators is reduced, the detection period is shortened, and a powerful technical support can be provided for detecting the proteusbacillus vulgaris in the industries of medical treatment, medical detection and the like.

The invention belongs to the field of biotechnology, and relates to a method for improving the thermal stability of L-amino acid deaminase, which is beneficial to Proteus mirabilis ( Proteus mirabilis ) L-amino acid deaminase enzyme mutant obtained by performing site-directed mutation, wherein the mutation site is Trp at position 120. Based on the protein spatial structure information, the present invention analyzes the spatial environment around the special amino acid site inside the structure and the interaction between amino acids including hydrogen bonds, ionic bonds, van der Waals, etc., and combines molecular biology techniques to analyze specific amino acid site modification, by rationally designing mutation sites, using site-directed mutation method to obtain L-amino acid deaminase mutants, which can increase the probability of obtaining positive mutant enzyme mutants, and also greatly reduce the work of mutant screening quantity. The invention improves the structural stability of the enzyme, and further helps to prolong the service life of the enzyme.

The invention belongs to the technical field of microbiological detection, and in particular, relates to primers, probes, a kit and a method for detecting proteus mirabilis. The invention discloses a set of primers and probes for detecting the proteus mirabilis, wherein the set of primers and probes comprise primers and probes for detecting four target sequences. The kit for detecting the proteus mirabilis comprises the primers and probes for detecting the proteus mirabilis. The primers and probes provided by the invention can accurately detect the proteus mirabilis, have good specificity and high sensitivity, and avoid generation of false positives; and the kit is low in cost, easy to use, and suitable for rapid screening for the proteus mirabilis.

The invention provides an oily sludge degrading bacterial strain Proteus mirabilis SB and its application; the bacterial strain is Proteus mirabilis, which was preserved in the China General Microorganism Culture Collection and Management Center on January 25, 2021, and the preservation number is CGMCC NO.21729. The present invention separates and obtains Proteus mirabilis SB from oily sludge, so the bacterial strain quickly becomes the dominant bacterial group after entering the oily sludge, effectively promotes the degradation of petroleum hydrocarbons, improves the physical properties of the soil, and enhances the biological activity of the soil; The strain can metabolize biosurfactant during growth and reproduction, and increase the degradation rate of microorganisms to petroleum hydrocarbons in oil sludge. The bacterial strain Proteus mirabilis SB involved in the present invention degrades petroleum pollutants in oily sludge in a short period of time, and the degradation rate of total petroleum hydrocarbons (TPH) in oily sludge reaches more than 70.5% within 2 weeks; The aromatics in the oil have a significant degradation effect, and the degradation rate of the aromatics in the total petroleum hydrocarbons in the oily sludge reaches 69.2% within 2 weeks.

The invention relates to acquisition and cloning and expression of a decarboxylase gene from proteus mirabilis (Proteus mirabilis) and belongs to the field of bioengineering. The decarboxylase is capable of removing carboxyl of 3,4-dihydroxyphenylpyruvic acid to generate 3,4-dihydroxybenzaldehyde. The decarboxylase has important industrial application value.