67results about How to "Wide host range" patented technology

The invention discloses COVID-19-S-RBD virus-like particles and vaccine, and preparation methods of the virus-like particles and the vaccine. pET28a-CuMVTT recombinant plasmid is constructed after a CuMVTT gene is used to connect pET28a plasmid; pFUSE-COVID-19-S-RBD recombinant plasmid is constructed by a COVID-19-S-RBD gene and pFUSE plasmid; the recombinant plasmid is respectively transferred into the expression strains of escherichia coli and the expression cell lines of 293 F cells; the expression strains of escherichia coli are cultivated, biomass is separated through centrifugation, andthe virus-like particles are obtained; COVID-19-S-RBD protein is obtained by cultivating the expression cell lines of the 293 F cells; and the virus-like particles are coupled to the COVID-19-S-RBD through a chemical coupling reagent SMPH. The virus-like particles and the vaccine can be easily obtained through bacteria culture; yield can be higher than that of chimeric expression; and thus, industrial production and fast immunity can be achieved.

The invention discloses an anti-pathogen hyperimmune yolk antibody, an AAV vector vaccine based preparation method and a preparation. The preparation method of the hyperimmune yolk antibody comprisesthe steps of selecting a first gene sequence containing an antigen protein fragment from a genomic sequence of a pathogen, and synthesizing a pathogen protective antigen gene through codon optimization; packaging a recombinant AAV vector vaccine carrying the antigen gene; and immunizing a healthy laying hen, and preparing the yolk antibody from a hen egg of the laying hen. According to the method,recombinant adeno-associated viruses with high safety, quick and simple packaging, low cost and stable expression are used as the vector vaccine for expressing protective antigen proteins of virusesor bacteria, the hen egg can be collected by immunizing the healthy laying hen once, the antivirus or antibacterial yolk antibody as an active component in yolk is extracted, and the antibody is natural immune globulin and can kill the viruses or bacteria and prevent viral or bacterial infection.

The invention belongs to the technical field of microorganism prevention and treatment, and particularly relates to a vibrio parahaemolyticus bacteriophage VP-HYP MCS-1 and bdellovibrio bacteriovorusHalobacterovoraxsp.MCS-1 composition and application thereof to prevention and treatment of prawn pathogenic vibrio parahaemolyticus infection. The vibrio parahaemolyticus bacteriophage is vibrio parahaemolyticus bacteriophage VP-HYPMCS-1, is preserved in China General Microbiological Culture Collection Center, and has a preservation number of CGMCC No.199693. The vibrio parahaemolyticus bacteriophage is a vibrio parahaemolyticus bacteriophage VP-HYPMCS-1. The bdellovibrio parahaemolyticus is bdellovibrio parahaemolyticus Halobacterovoraxsp.MCS-1, is preserved in the China General Microbiological Culture Collection Center (CGMCC), and has a preservation number of CGMCC No.199694. The invention also discloses a preparation method of the bdellovibrio parahaemolyticus strain. The virulent bacteriophage VP-HYP MCS-1 obtained through separation is high in host specificity and has cracking and killing effects on vibrio parahaemolyticus; the bdellovibrio bacteriovorus Halobacterium ovalax sp.MCS-1 is obtained through separation, the host spectrum of the bdellovibrio bacteriovorus Halobacterium ovalax sp.MCS-1 is wide, and the bdellovibrio bacteriovorus Halobacterium ovalax sp.MCS-1 has cracking and killing effects on various vibrios including vibrio parahaemolyticus; the vibrio parahaemolyticus bacteriophage and bdellovibrio bacteriovorus provided by the invention have good application prospects in prevention and control of prawn pathogenic vibrio parahaemolyticus infection.

Compositions and methods for the therapy and diagnosis of cancer, such as pancreatic cancer, are disclosed. Compositions may comprise one or more pancreatic tumor proteins, immunogenic portions thereof, or polynucleotides that encode such portions. Alternatively, a therapeutic composition may comprise an antigen presenting cell that expresses a pancreatic tumor protein, or a T cell that is specific for cells expressing such a protein. Such compositions may be used, for example, for the prevention and treatment of diseases such as pancreatic cancer. Diagnostic methods based on detecting a pancreatic tumor protein, or mRNA encoding such a protein, in a sample are also provided.

A recombined type 1 herpes simplex virus (Simplexvirus Herps simplexvirus 1rHSV-1Delta34.5 / lacZ), its preparation method and the application in antineoplastic are disclosed. The preparation process is: obtaining the beta-galactosidase expression box and purifying, constructing medium carrier IpFL, constructing medium carrier II pBL, constructing transfer carrier pIL and obtaining recombined virus. The apoptosis inhibiting gene icp34.5, a neurotoxic gene, is inactivateed by the reporter gene lacZ insertion. The recombined type 1 herpes simplex virus can only be replicated in the tumor cell, therefore, it is capable of killing tumor cell effectively, being safe for normal cell and tissue.