Recombinant virus expressing VP2 gene of bluetongue virus type 16 and construction method and application of recombinant virus
A bluetongue virus and recombinant virus technology, applied in the biological field, can solve problems such as bluetongue type 16 recombinant vaccines that have not yet been carried out, and achieve the effects of good genetic stability, wide host range and enhanced expression
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[0022] The following will be further described in conjunction with the accompanying drawings and examples. Unless otherwise specified, the present invention may adopt conventional techniques in the art. Unless otherwise explained, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this disclosure belongs.
[0023] The recombinant virus expressing the VP2 gene of type 16 bluetongue virus according to an embodiment of the present invention comprises a virus vector and a shuttle plasmid with the VP2 gene of type 16 bluetongue virus packaged in the virus vector, and the type 16 bluetongue virus VP2 The sequence of the gene is shown in SEQ ID NO.1.
[0024] Preferably, the viral vector is a vaccinia virus vector, and the shuttle plasmid is a vaccinia virus shuttle plasmid pSTKE.
[0025] Preferably, the upstream of the VP2 gene of type 16 bluetongue virus also has a kozak sequence.
[0026] The ...
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