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78 results about "Vp2 gene" patented technology

Recombination virus particles for expressing 2-typed porcine circovirus nucleocapsid protein Cap gene

The invention relates to construction and application of recombinant nucleocapsids of Cap genes of porcine circovirus expression type 2 nucleocapsid protein, belonging to the genetic engineering bacterin field. C-terminal gene fragments of porcine parvovirus (PPV) VP2 genes are cloned into a type 5 adenovirus shuttle vector of the human beings, and recombinant adenovirus rAd-deltaVP2 is obtained; deltaVP2 proteins are expressed successfully and highly efficiently and can be self-assembled into the nucleocapsids [PPV:VLPs]; the PPV VP2 nucleocapsids are used as antigen transport vectors and 165 to 200 sites of amino acid (deltaCap) genes of the porcine circovirus type 2 (PCV2) nucleocapsid proteins (Cap) are embedded into an N-terminal (deltaVP2) of the PPV VP2, and then recombinant adenovirus rAd-deltaCap-deltaVP2 is obtained; embedded VP2 (deltaCap-deltaVP2) proteins are expressed successfully and highly efficiently and can be self-assembled into nucleocapsids [PPV:VLP(PCV2)]. The invention also relates to application of the recombinant virus and recombinant PPV VP2 nucleocapsids of the expression Cap genes of the recombinant virus in the aspects of bacterin immunity and so on.
Owner:JIANGSU ACADEMY OF AGRICULTURAL SCIENCES

Method for producing porcine parvovirus antigen and its product

The invention discloses a method for producing a porcine parvovirus antigen and its product. The method comprises the following steps: porcine parvovirus capsid protein VP2 gene or optimized VP2 gene is cloned in a baculovirus carrier so as to obtain a transfer expression carrier; the constructed transfer expression carrier and baculovirus DNA are carried out cotransfection to obtain recombined baculovirus; the recombined baculovirus is used to infect insect host and cell; the infected insect host is cultured to express corresponding porcine parvovirus capsid protein; and the expressed antigen is ingathered and purified so as to obtain the porcine parvovirus antigen. The method adopts a baculovirus expression system to make safe and efficient porcine parvovirus antigen capsid particles in a domestic silkworm bioreactor; the prepared purified antigen by the method has high safety, and can be directly produced to vaccines for animal immunity. The method for producing porcine parvovirus antigen has the advantages of high expression efficiency, high immunization activity of the expressed antigen, low production cost, large scale production realization and the like.
Owner:THE INST OF BIOTECHNOLOGY OF THE CHINESE ACAD OF AGRI SCI

Immune colloidal gold test paper capable of simultaneously detecting canine distemper and canine parvo of foxes, raccoon dogs and minks and a method for preparing immune colloidal gold test paper

The invention discloses immune colloidal gold test paper capable of simultaneously detecting canine distemper and canine parvo of foxes, raccoon dogs and minks and a method for preparing the immune colloidal gold test paper. The test paper consists of a test paper box and a test paper tape, and the test paper tape is made by sequentially bonding a hard polyvinyl chloride lining plate, a nitrocellulose membrane, a long colloidal gold combination pad, a short colloidal gold combination pad, a sample pad and an absorption pad. VP2 gene monoclonal antibody- colloidal gold biomarkers resistant to mink-source canine distemper viruses and parvoviruses are sprayed on the long and short colloidal gold combination pads respectively. Two detection lines formed by rabbit anti-mink source canine distemper virus and parvovirus antibodies and a quality control line formed by a rabbit antimouse antibody are sprayed on the nitrocellulose membrane. The test paper is applicable to quick detection of pestilences of foxes, raccoon dogs and minks, and detection of two pestilences including canine distemper and canine parvo of foxes, raccoon dogs and minks can be simultaneously completed by the same test paper, and detection is simple, convenient, quick and accurate.
Owner:HEBEI NORMAL UNIVERSITY OF SCIENCE AND TECHNOLOGY +1

Virus-like particle recombinant protein of virus variation strain VP2 gene of infectious bursal disease

The invention relates to virus-like particle recombinant protein of a virus variation strain VP2 gene of an infectious bursal disease, belonging to the field of biologic pharmacy. The IBDV variation strain (AH1) VP2 gene is cloned, converted and transfected to obtain a recombinant baculovirus vBac-VP2; an infected Sf9 insect cell has specific fluorescence, and the antigen valence of the infected Sf9 insect cell is above 1.6*10<3>; the molecular weight of a recombinant VP2 protein is 53kDa, and the recombinant VP2 protein is in the state of virus-like particles; an indirect ELISA detection method established for an envelope antigen by the purified recombinant VP2 protein has good specificity and sensibility; an immune chicken can resist IBDV virulent attack, and the protection ratio achieves 100 percent. The novel virus-like particle recombinant VP2 protein prepared by the IBDV variation strain VP2 gene has high pertinence on the immune prevention of a current prevalent IBDV virulent strain and good practical value and popularization prospects.
Owner:JIANGSU ACADEMY OF AGRICULTURAL SCIENCES

Recombinant pseudo-rabies virus expressing swine parvovirus VP2 gene and vacine and its preparation method

The present invention relates to main structure gene VP2 of artificial constructed pseudorabies virus, PrV and porcine parvovirus, PPV. In the pseudorabies virus genome in which the main toxicity gene (TK) and virus generation nonessential gene (gG) are deleted the VP2 gene of porcine parvovirus can be site-specifically inserted to make it be positioned in strong late promoter downstream of pseudorabies virus, and the inserted exogenous gene coded protein has good immunogenicity, and can stimulate swine to produce protective immune reaction for resisting two virulent challenges of porcine parvovirus and pseudorabies virus. Said invention also includes recombinant pseudorabies virus, Hzau AVL-PRppvV-VP2, vaccine prepared by using it and its preparation method.
Owner:HUAZHONG AGRI UNIV

Anti-chicken infectious bursal disease recombinant protein subunit vaccine

The invention provides an anti-chicken infectious bursal disease (IBD) recombinant protein subunit vaccine. The vaccine is a fusion protein having high immunogenicity of Salmonella typhimurium flagellin and an infectious bursal disease virus (VP2). The above flagellin + VP2 fusion protein is obtained through the expression of a recombinant baculovirus containing a flagellin + VP2 gene by utilizing a Bac-to-Bac baculovirus expression system. The recombinant baculovirus obtained through the system has a short period, and the expressed flagellin + VP2 fusion protein has high immune protection force.
Owner:BEIJING ACADEMY OF AGRICULTURE & FORESTRY SCIENCES

Infectious bursal disease virus VP2 gene expressed recombinant newcastle disease LaSota attenuated vaccine strain

The invention discloses a VP2 gene restructuring Newcastle disease LaSota weak virus strain rLasota-VP2 of Infectious bursal disease virus, IBDV and appliance in the Newcastal disease virus,NDV and IBDV vaccine.
Owner:HARBIN VETERINARY RES INST CHINESE ACADEMY OF AGRI SCI

Preparation method of anti-canine parvovirus protein VP2 specific IgY

The invention discloses a preparation method of an anti-canine parvovirus protein VP2 specific IgY, which comprises the following steps: (1) designing a pair of primers according to CPV-VP2 gene sequence, carrying out PCR (polymerase chain reaction) amplification on the CPV-VP2 gene, connecting to a pMD18-T vector, transforming DH5alpha competent cell, carrying out blue-white screening, extracting the plasmid, carrying out digest enzyme digestion ion analysis, carrying out positive plasmid sequencing, and carrying out comparative analysis on the sequencing result; (2) expression and purification of VP2 protein in Escherichia coli: carrying out BamH I and Xho I double-enzyme digestion on the pMD18-T-VP2 and pET-32a,connecting to the target segment, constructing the pET-32a-VP2 expression vector, transforming Bal21(DE3)pLysS competent bacteria, carrying out enzyme digestion and PCR identification, optimizing the IPTG (isopropyl-beta-D-thiogalactopyranoside) induction concentration and time, carrying out mass induction expression, and purifying the recombinant protein by using a Ni<+> affinity column; and (3) preparation of anti-VP2-IgY antibody: immunizing a laying hen by using the purified VP2 protein, extracting the specific IgY antibody by using PEG 6000, and carrying out SDS-PAGE (sodium dodecyl sulfate polyacrylamide gel electrophoresis) analysis. The anti-VP2-IgY antibody extracted by the method can be well combined with the VP2 protein to carry out non-cross reaction on the degradation segment.
Owner:NORTHWEST A & F UNIV

Recombinant baculovirus expressing porcine parvovirus VP2 protein as well as preparation method and application

The invention discloses recombinant baculovirus expressing porcine parvovirus VP2 protein as well as a preparation method and an application. The method comprises the following steps: artificially synthesizing VP2 gene by referring to the VP2 gene sequence of a porcine parvovirus (PPV) isolate; with pFBDPHmHNM1P10eGFP plasmid as a skeleton, connecting the synthesized VP2 gene to the plasmid to obtain a baculovirus transfer vector pFBDPHm3VP2 and then obtain recombinant bacmid rBacmid-PPVP2; and transfecting the bacmid with sf9 cell to obtain recombinant baculovirus Ac-PPVP2. The recombinant baculovirus Ac-PPVP2 efficiently expresses PPV VP2 protein and successfully forms virus-like particles. The protein expressed by the recombinant baculovirus disclosed by the invention is used for preparing a subunit vaccine; and after the subunit vaccine immunizes an animal, the body can be induced to generate a specific immunoreaction, and the porcine body can be fully protected from the attack of strong poison of parvovirus.
Owner:HUAZHONG AGRI UNIV

Method of preparing porcine parvovirus virus-like particle subunit vaccine by using Escherichia coli expression system and application of method

The invention discloses an encoding gene of porcine parvovirus VP2 protein, a method of prokaryotically expressing VP2 protein virus-like particles, and application of the method in vaccine preparation. Sequences are optimized, VP2 gene is artificially synthesized, the synthesized gene is inserted into pET28a vector, the gene and chaperone protein plasmids are co-transferred to BL21(DE3) host bacteria, the VP2 protein and chaperone protein are co-expressed to promote correct folding of the VP2 protein. Experiments prove that recombinant bacteria expressed VP2 protein can be self-assembled in vitro and has good immunogenicity; by immunizing mice and guinea pigs with the virus-like particle subunit vaccine prepared with the VP2 protein expressed herein, it is possible to induce the production of a high level of hemagglutination inhibition antibodies and neutralizing antibodies, and the vaccine can prevent guinea pigs from being affected by strong porcine parvovirus. The recombinant bacteria according to the invention can be utilized to efficiently prepare porcine parvovirus virus-like particles, the production cost is low, operation is simple, and biosafety is better.
Owner:HENAN ACAD OF AGRI SCI +1

Recombinant turkey herpesvirus vaccine expressing infectious bursal disease virus VP2 protein and application thereof

The invention provides a recombinant turkey herpesvirus vaccine expressing infectious bursal disease virus VP2 protein and application thereof. According to the invention, on the basis of a Fosmid library continuously covering the whole genome of herpesvirus of turkey (HVT), a VP2 gene carrying a Pec composite promoter is inserted into site HVT053 and site HVT054 in the nonessential region of HVT replication by using Red ET homologous recombination technology and Gateway LR clone technology so as to obtain recombinant HVT (rHVT-VP2). The recombinant HVT (rHVT-VP2) can provide a complete protecting force on the infectious bursal disease virus and is applicable to prevention of the infectious bursal disease virus.
Owner:HARBIN VETERINARY RES INST CHINESE ACADEMY OF AGRI SCI

Recombinant rabies virus carrying two genes G and gene VP2 and application of recombinant rabies virus

The invention discloses a recombinant rabies virus rHEP-Flury(dG-VP2) carrying two genes G and a gene VP2. According to the recombinant virus, a rabies virus HEP-Flury strain serves as a framework, the VP2 gene shown as SEQ ID NO.1 is inserted into HEP-Flury, an additional gene G of the rabies virus is inserted, and the recombinant plasmid pHEP-Flury(dG-VP2) carrying the two genes G and the gene VP2 is obtained; finally, rescuing screening is carried out, and the recombinant rabies virus rHEP-Flury(dG-VP2) is obtained. The recombinant rabies virus rHEP-Flury(dG-VP2) carries the two genes G and expresses VP2 protein, a high rabies virus resisting antibody and canine parvovirus VP2 resisting antibody level can be generated through immune induction, the cost of vaccines for dogs can be reduced, and very good application and popularization prospects are achieved.
Owner:SOUTH CHINA AGRI UNIV

Recombinant porcine parvovirus-like particle and its preparation method and application

The invention discloses a recombinant porcine parvovirus-like particle. A preparation method of the recombinant porcine parvovirus-like particle comprises connecting a TAT protein transduction domain and an antigen VP2 gene for forming a PPV empty capsid protein, carrying out cloning in a baculovirus transfer vector to obtain a homologous recombinant vector, carrying out packaging to obtain a recombinant baculovirus containing a PPV VP2 protein and a TAT protein transduction domain, infecting an insect cell, and expressing a recombinant TAT-VP2 protein fused with the PPV VP2 protein and the TAT protein transduction domain to obtain the parvovirus-like particle (VLPs). A research result shows that the recombinant porcine parvovirus-like particle and an adjuvant infecting an animal can effectively stimulate the PPV-specific antibody production and improve a lymphocyte production capability of lymphocyte. Therefore, the recombinant porcine parvovirus-like particle can be used to develop a novel high-efficiency PPV subunit vaccine having good immune effects.
Owner:GUANGXI VETERINARY RES INST

Recombinant turkey herpesvirus and application thereof

The invention relates to the field of animal virology, and provides a recombinant turkey herpesvirus (rHVT-VP2), which is actually a brand-new recombinant turkey herpesvirus rHVT-VP2 obtained by combining an expression box of an avian infectious bursal disease virus VP2 gene and a turkey herpesvirus HVT. The recombinant turkey herpesvirus is also a recombinant vaccine of Marek's disease and avianinfectious bursal disease. By inserting the VP2 gene into a gene complex of the HVT under control of promoter, the rHVT-VP2 not only has fine immune protection for avian Marek's disease, but also haspersistent protective immunity of induced resistance of IBDV (infectious bursal disease virus) for commercial chickens with high IBDV material antibody level.
Owner:ZHAOQING DAHUANONG BIOLOGIC PHARMA +2

Recombinant Newcastle disease LaSota low virulent vaccine strain expressing infectious bursal disease virus VP2 gene

The present invention relates to one kind of recombinant Newcastle disease Lasota low virulent vaccine strain expressing infectious bursal disease virus (IBDV) VP2 gene, and is especially recombinant Newcastle disease Lasota low virulent vaccine strain rLasota-VP2. The present invention also discloses the preparation process of the recombinant Newcastle disease Lasota low virulent vaccine strain and the application of the recombinant Newcastle disease Lasota low virulent vaccine strain in preparing vaccine for preventing IBDV caused diseases.
Owner:HARBIN VETERINARY RES INST CHINESE ACADEMY OF AGRI SCI

Method for preparing gosling plague virus-like granules with escherichia coli system

The invention relates to a method for preparing gosling plague virus-like granules with an escherichia coli system for soluble expression of gosling plague virus VP2 protein. The method for soluble expression of gosling plague virus VP2 protein comprises the following steps: performing codon optimization on a gosling plague virus VP2 gene, performing site-specific mutagenesis, namely, mutating a codon AGA into CGC and mutating GGA into GGT, cloning to a pET-Sumo vector, establishing a recombinant expression vector pET-Sumo-VP2, transforming the pET-Sumo-VP2 into a prokaryotic expression bacterium, and inducing with IPTG (isopropyl beta-D-1-Thiogalactopyranoside) at 37 DEG C so as to obtain soluble recombinant VP2 recombinant protein; and performing digestion on the recombinant protein with a ULP enzyme, and purifying with a Ni column, thereby obtaining purified VP2 protein. Electron microscope results show that the gosling plague virus-like granules can be prepared from VP2 protein after digestion, and moreover, the purified VP2 protein has good reactogenicity and can be applied to preparation of subunit vaccines of gosling plague virus genetic engineering.
Owner:SHANDONG BINZHOU ANIMAL SCI & VETERINARY MEDICINE ACADEMY

Recombined chicken Marek's disease virus vaccine strain for expressing infectious bursaldisease virus VP2 gene and construction method and application of recombined chicken Marek's disease virus vaccine strain

The invention discloses a recombined chicken Marek's disease virus vaccine strain for expressing an infectious bursaldisease virus VP2 gene and a construction method and application of the recombined chicken Marek's disease virus vaccine strain, and belongs to the technical field of medicine or veterinary medicine. By means of the recombination and clone technology, a gene segment CAG-VP2 containing the infectious bursaldisease virus VP2 gene and a CAG promoter sequence is inserted in a US2 gene of the strain 814 of the chicken Marek's disease virus, a recombined cosmid with a CAG-VP2 expression frame inserted in a US2 gene is constructed, and the recombined chicken Marek's disease virus vaccine strain for expressing the infectious bursaldisease virus VP2 gene is obtained through salvation of the recombined cosmid. Research shows that the obtained vaccine strain has the same in-vitro replication ability as a parent virulent vaccine strain 814 and good hereditary stability, and can resist attacks of a very virulent MDV strain and a very virulent IBDV strain at the same time. It can be seen that the obtained recombined MDV vaccine strain can be used for preparing medicine for preventing or treating infectious bursaldisease and the chicken Marek's disease.
Owner:HARBIN VETERINARY RES INST CHINESE ACADEMY OF AGRI SCI

Specific primers and probe for fluorescence RT-PCR detection for bluetongue virus-16

The invention belongs to the field of virus detection, and relates to primers and a probe sequence for fluorescence RT-PCR detection for bluetongue virus serum-16. A real-time fluorescence RT-PCR detection method for BTV-16 comprises the following steps: adopting a Taqman technology, designing a group of specific primers which are only conservative in BTV-16 VP2 genes and a specific fluorescence labelling probe, and applying a fluorescence PCR instrument to detect a sample. Domestic and overseas BTV-16 viral nucleic acids can be specifically detected by using the fluorescence RT-PCR detection method established by the group of primers and the probe, and other serotypes of BTV nucleic acids cannot be detected. The method has the characteristics of being high in specificity, high in sensitivity, simple to operate, low in time consumption, high in efficiency and the like, and is capable of avoiding possible environmental pollution during a detection process.
Owner:YUNNAN ANIMAL SCI & VETERINARY INST

Universal PCR detection kit for cat and dog parvovirus

InactiveCN103160614AQuick and efficient detectionConvenient clinical diagnosis and treatmentMicrobiological testing/measurementMicroorganism based processesFecesPositive control
The invention discloses a universal PCR detection kit for rapid and high-efficient detection of cat and dog parvovirus. The method comprises designing a pair of primers in accordance with a conservation region of a vp2 gene of the cat and dog parvovirus, performing a PCR reaction by using excrement of detected animals as a template, comparing with negative and positive controls included in the kit, and determining whether the detected animals are infected with the parvovirus. The detection kit is simple, easy to use, high in sensitivity, and good in stability, and can be used for monitoring health conditions of the cat and the dog.
Owner:WITHYOU BIOTECH

Infectious bursal disease VP2 gene and application thereof

The invention relates to an infectious bursal disease VP2 gene, wherein the amino acid sequence of the gene is SEQ ID NO: 1. The infectious bursal disease VP2 gene sieved by the invention is used as a subunit vaccine in genetic engineering. The subunit vaccine prepared by the infectious bursal disease VP2 gene sieved by the invention makes SPF chickens which are 21 days old immune. After 21 days, chickens are of strong immunity, so that the immune chickens can resist the attack of virulent virus of infectious bursal disease.
Owner:QINGDAO VLAND BIOTECH INC +1

Recombinant yeast strain for expressing chicken IBDV (infectious bursal disease virus) VLPs (virus-like particles), protein expressed by recombinant yeast strain and application

ActiveCN105755015ATo achieve the purpose of clearing immunityFungiViral antigen ingredientsBiotechnologyHighly pathogenic
The invention discloses a recombinant yeast strain for expressing chicken IBDV (infectious bursal disease virus) VLPs (virus-like particles), a protein expressed by the recombinant yeast strain and an application. The recombinant yeast strain contains an optimized chicken IBDV VP2 gene sequence and can efficiently express a VP2 protein, and the expressed recombinant VP2 protein can be self-assembled into the VLPs. A vaccine is prepared from the chicken IBDV VLP protein expressed by the recombinant yeast strain to immunize chickens, and an experiment result indicates that the subunit peptide vaccine can effectively induce organisms to produce specific humoral immune response, so that the immunized chickens acquire 100% resistance to highly pathogenic vv IBDV fatal attack, residual viruses in the organisms can be cleared, and the purpose of sterilizing immunity is achieved. Therefore, one novel technical means is provided for prevention and control of chicken infectious bursal disease.
Owner:HARBIN VETERINARY RES INST CHINESE ACADEMY OF AGRI SCI

Mink parvovirus virus-like particle as well as preparation method and application thereof

The invention provides a mink parvovirus virus-like particle which is characterized in that VP2 gene of mink parvovirus is optimized according to insect cell optimal codons, the 5' end of the optimized VP2 gene is directly connected with the nucleotide sequence of polyhedron of a coding part and then is cloned into a transfer vector, and the mink parvovirus virus-like particle can be produced through a baculovirus / insect cell expression system. The mink parvovirus virus-like particle can be produced through the expression system in a safe, efficient and large-scale manner, and the obtained virus-like particle is high in titer and good in immunogenicity; both muscle immunization and oral immunization can induce a mink body to produce high-level specific antibody which can resist attack of virulent virus and well protect a mink, and a foundation is laid for the preparation of mink viral enteritis vaccine.
Owner:CHANGCHUN SR BIOLOGICAL TECH

Hydrophobia-canine distemper-canine parvovirus genetic recombination virus strain, construction method and application thereof

The invention discloses a hydrophobia-canine distemper-canine parvovirus genetic recombination virus strain, a construction method and application thereof. Through a homologous recombination method, agene replacement method and a gene insertion method, a full-length genome of a virus is subjected to gene rearrangement, and a rearranged hydrophobia virus genome plasmid with a canine distemper G gene and a canine parvovirus VP2 gene is constructed and is named as a hydrophobia-canine distemper-canine parvovirus, which is pcDSRV9-NG-eGFP+CDV N-PM+VP2-L genome plasmid; the hydrophobia-canine distemper-canine parvovirus is rescued through a reverse genetic method, and is an rSRV9+CDV N+VP2 gene recombinant virus; a gene nucleotide sequence of a recombination hydrophobia virus is SEQ ID NO:1. The recombination gene virus provided by the invention can be applied to researching a trigeminy oral vaccine for hydrophobia, canine distemper and canine parvovirus of canine and other wild animals soas to prevent three infectious diseases.
Owner:XINJIANG AGRI UNIV

Chicken infectious anemia virus VP1 and VP2 expression genetic recombination fowlpox virus living-vector vaccine

The invention discloses a chicken infectious anemia virus VP1 and VP2 expression genetic recombination fowlpox virus living-vector vaccine. On the basis of a fowlpox virus transfer vector Psy681, recombination infectious clone Psy681-VP1-LacZ-VP2 is constructed, and in a CEF cell, pSY681-VP1-LacZ-VP2 and a FVP strain gene are subjected to homologous recombination through a homologous arm to obtainrFPV-VP1-VP2. A rFPV-VP1-VP2 viral strain can induce the body to generate an effective antibody for resisting CAV, and an immunoprotection effect is achieved; and after obtaining a high-level effective antibody for resisting CAV, offspring of chickens can resist invasion of CAV strong viruses well, and the immunoprotection effect is achieved.
Owner:广州金苗动保科技有限公司

Recombined rhabdovirus for expressing porcine bocavirus VP2 protein and application thereof

The invention discloses a recombined rhabdovirus for expressing a porcine bocavirus VP2 protein and application thereof. A porcine bocavirus VP2 gene is amplified by PCR (polymerase chain reaction), and the nucleotide sequence of the porcine bocavirus VP2 gene is represented by SEQ ID NO.1. The amplified porcine bocavirus VP2 gene is inserted into BamHI and XhoI locuses of a rhabdovirus transmissible plasmid pFastBacTMHTB to construct a recombined transmissible plasmid pFastBac HTB-VP2; the recombined transmissible plasmid is converted into a DH10Bac competent cell comprising a rhabdovirus framework plasmid; the VP2 gene is integrated into the rhabdovirus framework plasmid Bacmid through homologous recombination; an sf9 cell is transfected by extracting the DNA of the recombined Bacmid to obtain the recombined rhabdovirus rBV-VP2 of which the preservation number is CCTCC V201401. A large quantity of VP2 protein and virus sample particles are expressed and produced in insect cells, and an effective PBoV VLPs subunit vaccine is developed.
Owner:HUAZHONG AGRI UNIV

Infectious bursal disease virus (IBDV) polyprotein gene (VP2/VP4/VP3), eukaryon expressing plasmid, DNA vaccine

A polymer protein (VP2 / VP4 / VP3) gene of infectious Fabricius bursa virus (IBDV) for intensifying the immunogenicity of vaccine and a recombinant carrier using pCI as eukaryon expression vector and containing said coding sequence are disclosed. The expression ability of said recombinant carrier increase by 10-40 times than normal eukaryon carrier. A DNA vaccine for infectious Fabricius bursa virus is also disclosed, which contains said eukaryon expression plasmid and immunological adjuvant and features high safety, stability and effect.
Owner:ZHEJIANG UNIV

Avian herpesvirus-based recombinant Infectious Bursal Disease vaccine

The present invention provides an avian recombinant herpesvirus modified by the presence of the cDNA encoding, the VP2 of the Delaware Variant E strain of IBDV, a subtype of IBDV serotype 1 strains. The present invention further provides an avian recombinant herpesvirus comprised of the VP2 gene of which the backbone virus is a Marek's disease vaccine strain, such as herpesvirus of turkeys. A poultry vaccine including the avian herpes recombinant virus described in the present invention can induce in chickens protective immunity against a variety of different subtypes of IBDV.
Owner:ZEON CORP

Recombinant herpesvirus of turkey (HVT) and preparation method thereof

The invention belongs to the technical field of biology and in particular discloses a recombinant herpesvirus of turkey (HVT). The recombinant HVT is capable of simultaneously expressing NDV and IBDVprotective antigen genes, and is a bacterial artificial chromosome (BAC) based on the HVT. The construction method comprises the following steps: inserting a VP2 gene expression cassette of an infectious bursal disease virus LX strain under regulation of a cytomegalovirus promoter CMV into a UL45-46 non-essential region of the HVT genome by utilizing a gene recombination technology; and insertingan F gene expression cassette of a newcastle diseases virus PX02 / 3 strain under regulation of a chicken beta-actin promoter pec into another non-essential region US1-10 of the HVT genome. The recombinant herpesvirus of turkey is a recombinant triplet poultry vaccine candidate strain capable of controlling Marek's disease of chickens and effectively resisting Newcastle disease and infectious bursaldisease.
Owner:BEIJING ACADEMY OF AGRICULTURE & FORESTRY SCIENCES

Recombinant lactobacillus casei for expressing infectivity pancreatic necrosis virus VP2 protein and production method thereof

The invention provides a recombinant Lactobacillus casei for expressing the infectious pancreas necrosis virus (IPNV) VP2 protein and a preparation method thereof. According to the whole gene sequence of the IPNV VP2 protein and the gene fusion characteristic of expression vector plasmid, a pair of primers are designed to perform PCR to obtain target segment of 1095bp containing the main antigen site of the IPNV VP2 protein, the IPNV VP2 protein obtained through amplification is linked to the surface expressed carrier plasmid pPG1 and enters the cell of the host strain Lactobacillus casei 393 through electrotransformation, and the Lactobacillus casei containing positive recombinant plasmid pPG1-IPNV VP2 is named pPG1-IPNV VP2 / L. casei 393. The recombinant pPG1-IPNV VP2 / L. casei 393 is expressed under the induction of lactose. In the invention, the Lactobacillus casei expression carrier system of the IPNV VP2 protein is constructed and the target protein of around 40 KDa containing the main antigen site of the IPNV VP2 protein is expressed, and according to the Western blot experiment and indirect immunofluorescence experiment, the expressed foreign protein is capable of reacting with the IPVN immune serum, which shows that the recombinant VP2 protein and the IPNV natural antigen have the same antigenicity.
Owner:NORTHEAST AGRICULTURAL UNIVERSITY

Subunit vaccine of PPV (porcine parvovirus) disease and preparation method of subunit vaccine

The invention provides a subunit vaccine of PPV (porcine parvovirus) disease and a preparation method of the subunit vaccine. PPV virus-like particles are provided firstly and produced by steps as follows: optimized VP2 gene and IL-15 (interleukin-15) gene are subjected to fusion expression, and the PPV virus-like particles are produced by a baculovirus / insect cell expression system. The PPV virus-like particles are mixed with a preservative and an adjuvant, and the PPV disease subunit vaccine is prepared. The PPV disease vaccine has the advantages of high yield, low production cost, high immunogenicity, good safety and the like, facilitates large-scale production, and has good immunization and prevention effects on PPV disease.
Owner:CHANGCHUN SR BIOLOGICAL TECH
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