Recombinant lactobacillus casei for expressing infectivity pancreatic necrosis virus VP2 protein and production method thereof
A technology of Lactobacillus casei and necrosis virus, which is applied in the field of recombinant Lactobacillus casei and its production method, and the preparation of biological products, which can solve the problems of no biological activity, inability to transfer antigenic components, and pyrogens caused by toxic cells
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[0036] The present invention is described in more detail below in conjunction with accompanying drawing example:
[0037] 1. Construction of IPNV VP2 gene Lactobacillus casei expression vector
[0038] The recombinant plasmid pMD18-T-IPNV VP2 and plasmid pPG1 were extracted from Escherichia coli pMD18-T-IPNV VP2 / JM109 and Lactobacillus casei393 containing plasmid pPG1 with a plasmid DNA mini-extraction kit. After pPG1 and pMD18-T-IPNV VP2 were double digested, the target fragments were recovered and purified. The recovered purified products of pPG1 and VP2 were ligated, and the ligated products were electrotransformed into competent cells Lactobacillus casei 393, and the Lactobacillus casei containing the positive recombinant plasmid pPG1-IPNV VP2 was named pPG1-IPNV VP2 / L.casei 393.
[0039] 2. Induced expression of IPNV VP2 protein in Lactobacillus casei
[0040] Inoculate a single colony of pPG1-IPNV VP2 / L.casei 393 into 5mL MRS liquid medium for overnight culture to acti...
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