55 results about "Oral immunization" patented technology

The invention discloses a preparation method of a porcine epidemic diarrhea recombinant adenovirus vaccine. The preparation method provided by the invention comprises the following steps of inserting a DNA sequence of a zone S1 of a porcine epidemic diarrhea virus (PEDV) into an adenovirus shuttle plasmid pShuttle-CMV to obtain pShuttle-CMV-S1, carrying out linearization of the pShuttle-CMV-S1, transforming the linear pShuttle-CMV-S1 into a BJ5183 competent cell containing pAdEasy-1, carrying out homologous recombination, carrying out enzyme digestion, carrying out AD-293 cell transfection, carrying out packaging to obtain a recombinant adenovirus rAd-S1, and carrying out purification, amplification and sub-packaging. After oral immunization, the porcine epidemic diarrhea recombinant adenovirus vaccine can induce generation of mucosal immunity thereby preventing porcine epidemic diarrhea (PED) well.

The invention belongs to the technical field of vaccine preparation, and particularly relates to an attenuated strain of a grass carp reovirus II and an application of the attenuated strain. The preservation number of the attenuated strain is CCTCC NO:V201867. A live vaccine prepared from the attenuated strain can prevent diseases caused by the grass carp reovirus II; the immune protection effectfor the same genotype reaches 95% or above during injection use, reaches 65% or above during soaking immunization and reaches 40% or above during oral immunization; no stress reaction is generated toa body; the risk of virulence return is avoided; the vaccine can be applied to prevention of hemorrhagic diseases, especially viral hemorrhagic diseases of grass carp and black carp caused by the grass carp reovirus II; the morbidity of the hemorrhagic diseases of the grass carp and the black carp is reduced; and the survival rate of the grass carp and the black carp is increased.

The invention relates to recombinant bacillus subtilis for expressing S protein of transmissible gastroenteritis of swine virus (TGEV), and belongs to the fields of biotechnology and genetic engineering. According to the recombinant bacillus subtilis disclosed by the invention, a TGEV S protein recombinant bacillus subtilis integrated expression plasmid pIncotGSR is successfully constructed, and the expression plasmid is successfully transformed into bacillus subtilis WB800 through electric shock. Based upon Western-blot verification, the TGEV S protein can be successfully expressed in the recombinant bacillus subtilis. An effective TGEV specific immune response level can effectively generate in the body of a one-month-old piglet through stimulation of oral immunization. The recombinant bacillus subtilis is expected to be developed as a genetic engineering oral vaccine for preventing transmissible gastroenteritis of swine.

The invention discloses a rabbit coccidiosis vaccine and application thereof. The rabbit coccidiosis vaccine comprises 100-800 pieces of medium eimeria, 200-1600 pieces of large eimeria and 100-800 pieces of eimeria intestinalis. The vaccine is scientific and reasonable in composition, low in cost, free of drug residue after use, drug tolerance and environment pollution, high in immunogenicity andsafe to use; after oral immunization, rabbits can effectively resist infection of 1x105 medium eimeria, 5x104 large eimeria and 3x103 eimeria intestinalis, and the vaccine can be applied in preparingdrug preparation for preventing rabbit coccidiosis.

The invention relates to the technical field of biology, in particular to probiotics cloned strain of integrated four-cope F18 pili operon gene and two-copy F4 pili operon gene and a construction method. The method comprises the following steps: constructing pTargetT-X::PtetF18 and pTargetT-X::PtetF4 recombinant plasmid; and obtaining nonresistant four-cope F18 pili gene and two-copy F4 pili geneintegration cloned strain through construction of the probiotics cloned strain of the four-copy functional F18 pili operon gene and the two-copy F4 pili operon gene. The probiotics cloned strain has the benefits that recombinant bacteria can effectively improve the adhesion property to pig intestinal passage epithelial cell; mice subjected to oral immunization can produce immune serum, namely anti-F18 pili IgG antibody and anti-F4 pili IgG antibody; and serum of the mice subjected to oral immunization can obviously reduce adhesion to pig intestinal passage cell line IPEC-J2 by F18+ strain andF4+ strain.

The invention relates to a flavobacterium columnare transgenic engineering oral vaccine. Preparation steps are as follows: (1) analysis of a mature peptide fragment sequence of a protective antigenicgene, namely lip, of flavobacterium columnare; (2) construction of shuttle plasmids; (3) screening of recombinant strains; (4) culture of the recombinant strains; and (5) preparation of the oral vaccine. The vaccine is an oral vaccine and is inoculated in an oral immunization mode, the oral vaccine is convenient to use compared with injection immunization and immersion immunization vaccines, morelabor force is saved, and the flavobacterium columnare transgenic engineering oral vaccine has the advantage of being not limited by the size of fish bodies.

The invention discloses a porcine parvovirus oral vaccine composition which includes the components a) and b), is basically composed of the a) and b), or is composed of the a) and b), wherein the component a) is viroid particles formed by self-assembling porcine parvovirus VP2 protein expressed by Kluyveromyces marxianus recombinant engineering bacteria; b) pharmaceutically or veterinarily acceptably auxiliary material for oral-taking preparations. The invention also discloses a preparation method and an application of the porcine parvovirus oral vaccine composition. The porcine parvovirus oral vaccine composition has excellent immunogenicity. An orally immunized mouse can generate protective antibodies which have high titer. The oral vaccine composition has high safety and low productioncost, can be produced to an amplified scale and has simple operation, and can be used for preventing and alleviating clinical symptoms related to PPV infection.

The invention discloses a preparation method of a porcine epidemic diarrhea virus genetic engineering subunit oral vaccine. The method comprises the following steps of: 1) synthesizing an M gene; 2) constructing an expression vector; 3) performing protein induction expression; 4) carrying out recombinant strain preservation experiment; and 5) preparing the vaccine. The oral immunization has the advantage of effectively stimulating local immune cells of the intestinal tract to generate secretion type IgA, is especially applicable to the infectious disease of the intestinal mucosa, and avoids stress and vaccine absorption problems resulting from conventional vaccine injection; and as a safe and non-toxic live vector system capable of living on the intestinal mucosa, the lactococcus lactis is the most suitable vector system. The oral vaccine performs antigen presentation through the gastrointestinal mucosa, and produces immune reaction similar to conventional injection through different immune pathways.

The invention discloses a method for preparing an animal oral immunization vaccine vector. The preparation method comprises the following steps: taking green vegetables as a vaccine vector, and injecting vaccine liquid in a first vaccine dose on the vaccine vector; sterilizing the vaccine vector, naturally airing, refrigerating and preserving, thereby obtaining the product. The animal oral immunization vaccine vector prepared according to the preparation method disclosed in the invention is prepared by enabling the green vegetables that are often fed to animals to carry the immunized vaccine liquid. Therefore, when the green vegetables are broken by teeth of the animals, the stem chambers containing the vaccine liquid are broken in animal mouths, the vaccine liquid flows out, the vaccine liquid is in sufficient contact with the animal mouths by virtue of repeated chewing of the animals, and the expected mucosal immunity is achieved. After the residues of the green vegetables are directly eaten, poisoning to the outside is avoided, and nutrition needed by the animals is supplemented.

The invention discloses a subunit vaccine for preventing Glasser's disease and a preparation method thereof. According to a prokaryotic expression method, an outer membrane protein Omp16 with immunogenicity is expressed by cloning a haemophilus parasuis (Haemophilus parasuis, H. parasuis) strain (culture collection number: CGMCC NO. 11145, patent application number for invention: CN105524857A). The haemophilus parasuis subunit microsphere vaccine is prepared by taking the protein Omp16 as an antigen and coating the protein Omp16 by taking chitosan, olive oil and sodium alga acid as materials. For mice subjected to injection and oral immunization by using the vaccine, the immune protective rates of H. parasuis serum type 5 attack are respectively 80% and 60%.

The invention provides a bivalent nucleic acid vaccine against bronchitis and a preparation method and application thereof. According to the invention, the core expression region of the bivalent nucleic acid vaccine is only 1491 bp; a secretory signal peptide is introduced before an expression sequence; a Kozak sequence is introduced into the expression sequence; plasmids containing avian promotersequences are selected; hydrophobic regions which are not easy to become antigens are removed; M41 and H120 characteristic proteins are spliced, and a linker sequence is arranged between and connected with the M41 and H120 characteristic proteins, so generation of neutralizing antibodies aiming at the two toxic strains can be stimulated, and the defect of poor crossover of immunity to infectiousbronchitis viruses is overcome; Salmonella typhimurium is selected as a vector, and Salmonella typhimurium has no hidden infection dangers to a host, but can support long-term expression of nucleic acid vaccines to achieve a long-term immune effect; and an oral immunization mode is adopted for the vaccine, and the vaccine can be added into a feed, and is convenient to use and superior to a nasal dripping or injection immunization mode. During preparation of the vaccine, a culture collected after fermentation is directly used as a vaccine, so the steps of protein purification, endotoxin removaland the like are reduced, cost is saved, and large-scale production is facilitated.

The invention discloses an application of an immunostimulating compound in preparing fish immune preparation, and the immune preparation is an oral immune preparation or a bath immune preparation. The advantage of the present invention is that: the oral immune preparation or bath immune preparation made of the immune stimulating compound ISCOMs can not only increase the antibody level, but also improve the proliferation and transformation ability of T lymphocytes and induce immune protection. When immunized with the same dose, the antibody titer of ISCOMs group was significantly higher than that of no adjuvant group and ISM1312 adjuvant group. Similarly, oral immunization with ISCOMs can not only increase the antibody level, but also improve the proliferation and transformation ability of T lymphocytes and induce immune protection.