91 results about "Swine Transmissible Gastroenteritis" patented technology

The invention relates to a porcine transmissible gastroenteritis and epidemic diarrhea combined live vaccine and a preparation method of the porcine transmissible gastroenteritis and epidemic diarrhea combined live vaccine. The porcine transmissible gastroenteritis and epidemic diarrhea combined live vaccine is prepared by performing virus amplification on a swine testicular cell line (ST cells) or an African green monkey kidney cell line (Vero cells) by using a self-attenuated and preserved transmissible gastroenteritis virus SD/L strain and a self-attenuated and preserved porcine epidemic diarrhea virus LW/L strain, and carrying out the steps of harvesting, uniformly mixing, freeze-drying and the like. The porcine transmissible gastroenteritis and epidemic diarrhea combined live vaccine can effectively prevent two diseases namely swine transmissible gastroenteritis and epidemic diarrhea.

The invention provides a triple live vaccine for a swine transmissible gastroenteritis virus, a swine epidemic diarrhea virus and a swine rotavirus and a preparation method thereof. The content of the three viruses is not less than 107.5 TCID50 (Tissue Culture Infectious Dose 50)/mL, and the volume ratio is 1:1:1. The triple live vaccine provided by the invention solves the problem that a multiple vaccine for effectively preventing and treating such three diseases as swine transmissible gastroenteritis, swine epidemic diarrhea and the swine rotavirus is not available on the current market, and especially realizes the prevention and control on the swine rotavirus. Compared with the existing method of inoculating with three simplex vaccines to prevent such three transmissible diseases, the triple live vaccine provided by the invention is economical to use, simplifies the immunization procedure and lowers the epidemic prevention cost, thereby providing a new simple and convenient immunization way for farms in China.

A yolk antibody for pig's infective enterogastritis virus is prepared through reproducing the said virus on pig's testis cell, applying it as immunogen to the layer, collecting yolk and purifying. When it is applied to pig, the comparison between experimental and reference groups shows that it has high effect on preventing and cuting pig's infective enterogastritis.

The invention relates to a test paper card for detecting porcine transmissible gastroenteritis virus antibody, preparation and detection method thereof, and belongs to the field of immunological detection. The test paper card includes a jammed case and a test strip, and the test strip includes a bottom plate and sequentially lapped and pasted on the Absorbent pad, detection pad, binding pad and sample pad on the base plate; the detection pad is a nitrocellulose membrane with a quality control line C and a detection line T, the quality control line C is coated with goat anti-mouse monoclonal antibody, and the detection line T is Coated with inactivated porcine transmissible gastroenteritis virus; the binding pad is a glass cellulose membrane embedded with time-resolved fluorescent microsphere-labeled anti-E2 protein monoclonal antibody; the sample pad is dried glass after soaking in the sample treatment solution Cellulose film. The test paper card prepared by the invention has better stability and higher sensitivity, and can achieve the purpose of semi-quantitative detection through fluorescence signal analysis.

The invention discloses a combined live vaccine of transmissible gastroenteritis virus of swine (TGEV) and porcine epidemic diarrhea virus (PEDV) and a preparation method thereof. An attenuated swine transmissible gastroenteritis virus HB08 and an attenuated porcine epidemic diarrhea virus ZJ08 which are self-separated, attenuated and stored respectively undergo viral multiplication on ST cells and VeroE6 cells, and seedling and freeze drying are then carried out by adding a freeze-drying protective additive into a virus solution which is qualified after inspected. The two diseases, transmissible gastroenteritis of swine and porcine epidemic diarrhea virus, which are epidemic in clinic at present, can be effectively prevented by the use of the combined live vaccine.

The invention relates to a preparation and application method for treating a swine viral diarrhea biological agent, and belongs to the technical field of biological agents. The method specifically comprises the following steps of: (1) preparing for immunogen preparation, namely, getting swine transmissible gastroenteritis and porcine epizootic diarrhea duplex inactivated vaccine which consists of two viral protein; (2) performing immune procedure; (3) detecting valence of antibody; (4) purifying an egg yolk antibody; and (5) identifying the egg yolk antibody. The preparation and application method has the advantages that the water diluting method is performed and the method is safe, reliable, out of pollution, and low in cost; the treated high-immunity egg can gain the egg yolk antibody with high activity, purity and output and stable performance; the operation method is simple, feasible, and reliable; the industrial production can be carried out conveniently.

The invention belongs to the technical field of animal virology and animal infectious diseases and specifically relates to a primer and a multiple RT-PCR method for identifying and detecting swine diarrhea coronavirus. The swine diarrhea coronavirus relates to classic strain and variant strain of swine epidemic diarrhea virus, swine delta coronavirus and swine transmissible gastroenteritis. The invention discloses a multiple RT-PCR primer and a detecting method for simultaneously detecting the classic strain and variant strain of swine epidemic diarrhea virus, swine delta coronavirus and swinetransmissible gastroenteritis; the classic strain and variant strain of swine epidemic diarrhea virus, swine delta coronavirus and swine transmissible gastroenteritis which seriously impair pig industry can be simultaneously detected and distinguished through once experiment; a new, quick and effective detecting method is supplied for identifying the viruses and diagnosing the clinic diseases; the detection time can be shortened; and the detection cost can be lowered.

The invention provides an RT-LAMP (reverse transcription loop-mediated isothermal amplification) detection method for transmissible gastroenteritis of swine TGE. The RT-LAMP detection method comprises the following steps: 1) designing an LAMP primer by using an online software according to a conserved region of a TGEVN (TGE Virus N) gene in a GenBank; 2) preparing a virus solution for use at the temperature of 80 DEG C below zero; 3) extracting RNA (Ribose Nucleic Acid) of TGEV; 4) taking the RNA of the TGEV obtained in the step 3) as a template for RT-LAMP reaction; and 5) detecting an RT-LAMP reaction result with agarose gel. Compared with the conventional method, the RT-LAMP detection method is simple in operation, quick, and high in efficiency, specificity and sensitivity.

The invention discloses a fluorogenic quntitative PCR kit for detecting swine transmissible gastroenteritis virus. The fluorogenic quantitative PCR kit comprises primer pairs showed in SEQ ID NO: 1-2 for amplifying swine transmissible gastroenteritis virus genes. According to the kit disclosed by the invention, the swine transmissible gastroenteritis virus can be detected accurately and effectively, and the kit has the advantages of strong specificity, high sensitivity, short consumed time, high detecting speed and good application prospect.

The invention discloses a TaqMan fluorogenic quantitative PCR kit and a detection method for porcine deltacoronavirus. The kit comprises specific primers and a probe, wherein primer sequences are as follow: the upstream primer is 5'-ACGTCGTAAGACCCAGCATC-3' and the downstream primer is 5'-CCCACCTGAAAGTTGCTCTC-3', and a probe sequence is 5'-FAM-GTATGGCTGATCCTCGCATCATGGC-BHQ1-3'. A gene part fragment648bp of the porcine deltacoronavirus is expanded, a lowest detection limit of the TaqMan real-time fluorogenic quantitative PCR disclosed by the invention is 26.6copies/mu L, and the detection results of the TaqMan real-time fluorogenic quantitative PCR to swine transmissible gastroenteritis virus, porcine epidemic diarrhea virus, porcine kobuvirus, porcine reproductive and respiratory syndromevirus as well as foot and mouth disease virus are all feminine. The TaqMan probe real-time fluorogenic quantitative PCR method for detecting the porcine deltacoronavirus (PDCoV) disclosed by the invention has the advantages of strong specificity, high sensitivity and good inter-batch and intra-batch repeatability. When the TaqMan probe real-time fluorogenic quantitative PCR method disclosed by theinvention are used for detecting 194 clinical pig manure samples, a result shows that a PDCoV positive rate is 22.1% and is about one time higher than a 11.9% positive rate of detecting the clinicalpig manure samples by general RT-PCR.

The invention provides an RT-qPCR detection method, primers and TaqMan probe for detecting a swine transmissible gastroenteritis virus N gene. Particularly, the invention provides a set of RT-qPCR detection method for detecting the swine transmissible gastroenteritis virus gene and a forward primer shown in SEQ ID No.1 in a sequence table, a reverse primer shown in SEQ ID No.2 in the sequence table, and a TaqMan probe sequence shown in SEQ ID No.3 in the sequence table. The invention provides an RT-qPCR detection method for detecting the swine transmissible gastroenteritis virus N gene on the basis of the primers and the TaqMan probe. The detection method has excellent detection sensitivity, and in the case of 1*10<5>copies/microliter to 1*10<1>copies/microliter, Ct values and fluorescence values show typical amplification curves. At the same time, the detection method has good repeatability, strong specificity and convenience in operation.

Belonging to the technical field of disease prevention and treatment, the invention particularly discloses a pharmaceutical composition for preventing or treating diseases caused by coronaviruses and/or rotaviruses. The invention for the first time studies and finds that taurine can prevent or treat a series of diseases caused by coronaviruses or rotaviruses, like porcine epizootic diarrhea, swine transmissible gastroenteritis, rotaviruses caused rotavirus diarrhea and the like. Therefore, on the basis, the invention develops a pharmaceutical composition containing taurine, the pharmaceutical composition combines taurine, a vitamin compound and a flavoring agent, and the nutrient intake of animals is increased and the resistance of the body itself is strengthened to realize the purpose of assisting taurine in efficient treatment of diseases.

The invention discloses a swine-derived single-chain antibody capable of resisting swine transmissible gastroenteritis viruses and a preparation method of the swine-derived single-chain antibody. The swine-derived single-chain antibody is formed by linking a heavy-chain variable region with a light-chain variable region through a short linker peptide, wherein an amino acid sequence of the light-chain variable region is shown as SEQ ID No.1, and an amino acid sequence of the heavy-chain variable region is shown as SEQ ID No.2. The swine-derived single-chain antibody capable of resisting the swine transmissible gastroenteritis viruses is about 28 kDa in molecular weight and capable of specifically recognizing the swine transmissible gastroenteritis viruses and can be used for further blocking infection and invasion of the swine transmissible gastroenteritis viruses.

The invention discloses a multiplex RT-PCR (reverse transcription-polymerase chain reaction) primer probe group for real-time fluorescent quantitative detection of four porcine epidemic diarrhea viruses. The multiplex RT-PCR primer probe group comprises an upstream primer, a downstream primer and a specific fluorescent probe of a porcine epidemic diarrhea virus M gene, an upstream primer, a downstream primer and a specific fluorescent probe of a swine transmissible gastroenteritis virus S gene; an upstream primer, a downstream primer and a specific fluorescent probe of a porcine rotavirus VP6 gene; and an upstream primer, a downstream primer and a specific fluorescent probe of a porcine D-type coronavirus M gene. The kit assembled on the basis of the primer probe group has the advantages of high sensitivity, high specificity, low pollution and real-time detection, and provides a reliable technology and product for early warning, early diagnosis and prevention and control monitoring of clinical diarrhea virus in a first-line pig farm.

The invention discloses a swine transmissible gastroenteritis S/N protein fusion gene, recombinant lactococcus lactis and application. The invention provides an antigen fusion gene obtained by connecting A and D antigen loci in swine transmissible gastroenteritis virus (TGEV) S protein and an N321 antigen locus in N protein and a streptococcus pyogenes cell wall anchoring sequence, and a nucleotide sequence of the antigen fusion gene is shown as SEQ ID No. 1. The invention further provides an expression vector containing the antigen fusion gene and the recombinant lactococcus lactis strain containing the recombinant expression vector. The recombinant lactococcus lactis strain is induced by using nisin, the fusion gene can be expressed on the surface of the bacterial cell wall. Immunoblotting experiments indicate that the expressed recombinant protein can react with TGEV immune serum and has the same antigenicity with TGEV natural antigens, and the recombinant protein can be prepared into safe and effective mucous immune live vaccines for preventing and treating swine transmissible gastroenteritis.

The invention relates generally to the field of virology, and relates to the identification and characterization of the targets involved in the evasion strategy of viruses and to the use thereof in methods to identify anti-viral compounds. More in particular to identify compounds which are modulators of myosin light chain kinase (MLCK), a target within said entry and immune-evasion strategy. Other aspects of the invention are directed to anti-viral compounds identified using the models and methods of the present invention, as well as to the use thereof in treating viral infections, such as for example caused by the feline infectious peritonitis virus (FIPV), a coronavirus which belongs to an antigenic group which comprises in particular feline enteric coronavirus (FECV), canine coronavirus (CCV), swine transmissible gastroenteritis coronavirus (TGEV), porcine respiratory coronavirus (PRCV) and human coronavirus (HCV), and which induces, in a host-dependent manner, a range of symptoms which range from mild enteritis to the severe debilitating disease, and, in some cases, up to death. In a particular aspect the present invention provides the use of MLCK inhibitors for the treatment of feline infectious peritonitis (FIP).