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Yolk antibody and antigen of pig's infective enterogastritis virus and its preparing process

A yolk antibody, infectious technology, applied in viral peptides, egg-derived immunoglobulins, peptide sources, etc., to achieve obvious therapeutic effects

Inactive Publication Date: 2002-12-04
INST OF ZOOTCHNICS & VERTERINARY SCI BEIJING ACAD OF AGRI & FORESTRY SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

But there is no report about the yolk antibody of porcine transmissible gastroenteritis virus

Method used

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  • Yolk antibody and antigen of pig's infective enterogastritis virus and its preparing process
  • Yolk antibody and antigen of pig's infective enterogastritis virus and its preparing process
  • Yolk antibody and antigen of pig's infective enterogastritis virus and its preparing process

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Example 1 Porcine Transmissible Gastroenteritis Virus (TGEV) Proliferation and Antigen Preparation

[0035]In this experiment, porcine testicular cells (ST) were selected as infected cells, and an appropriate amount of trypsin was added during virus infection to promote the adaptation and infection of the virus. The test results showed that the virus infection titer was significantly increased, and obvious cytopathic changes occurred. 1. Materials 1.1 TGEV isolates come from China Veterinary Drug Control Institute. 1.2 Newborn piglets 1-day-old colostrum-free piglets came from a pig farm in the suburbs of Beijing with no history of TGEV. 1.3 Porcine testicular cells (ST) were purchased from China Veterinary Drug Control Institute. 1.4 Cell culture medium MEM basal medium was purchased from GIBCO BRL Company, and neonatal calf serum was purchased from Hyclone Company. 2. Method 2.1 TGEV proliferates in pigs. After the 1-day-old piglets that did not eat colostrum were b...

Embodiment 3

[0044] Example 3 TGEV egg yolk antibody treatment test

[0045] 12 1-day-old piglets that did not eat colostrum were fed with milk, observed for 1 day, and randomly divided into 4 groups, namely: treatment group (4 pigs) for challenge, no treatment group (4 pigs), and no treatment group (4 pigs). Poison group (2 heads), no treatment and no attack group (2 heads). The virus used in the attack was ultrafiltered TGEV diluted 10 times with 0.01MPBS and then directly orally administered. The egg yolk antibody used in the treatment was a purified sample. The titer of the egg yolk antibody determined by the agar diffusion test was 1:64. and mortality rate to judge the therapeutic effect of TGEV yolk antibody.

[0046] The test results showed that the piglets in the two non-challenge groups grew normally without diarrhea symptoms, and whether the yolk antibody was fed or not had no effect on their growth; while the piglets in the two challenge groups all had severe diarrhea symptoms,...

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Abstract

A yolk antibody for pig's infective enterogastritis virus is prepared through reproducing the said virus on pig's testis cell, applying it as immunogen to the layer, collecting yolk and purifying. When it is applied to pig, the comparison between experimental and reference groups shows that it has high effect on preventing and cuting pig's infective enterogastritis.

Description

technical field [0001] The invention relates to an antibody and antigen of porcine transmissible gastroenteritis virus and a preparation method thereof. Background technique [0002] Porcine transmissible gastroenteritis virus (TGEV) is a member of the Coronaviridae family and the genus Coronaviridae. TGEV virus can infect pigs of all ages, especially causing vomiting, severe diarrhea and high mortality (usually 100%) in piglets under the age of two weeks. , often occurs repeatedly and is popular in pig farms, bringing great harm to the pig industry. (Pensaert M B. Disease of Swine. Seventh edition. Iowa State University Press. USA. 1992, 305-338). [0003] At present, there is no particularly effective means for the prevention and treatment of the disease. Viable treatment is symptomatic only, reducing starvation, dehydration, and acidosis. There are many reports on immune prevention of TGEV, including active immunization using virulent or attenuated TGEV, and passive i...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/17C07K16/02
Inventor 王金洛徐福州杨兵宋维平赖平安秦泽云
Owner INST OF ZOOTCHNICS & VERTERINARY SCI BEIJING ACAD OF AGRI & FORESTRY SCI
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