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RT-LAMP (reverse transcription loop-mediated isothermal amplification) detection method for transmissible gastroenteritis of swine TGE

A technology of RT-LAMP and detection method, which is used in the determination/inspection of microorganisms, resistance to vector-borne diseases, biochemical equipment and methods, etc., to achieve the effect of simple operation and high sensitivity

Inactive Publication Date: 2013-02-06
GUANGDONG WENS DAHUANONG BIOTECH +1
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

In recent years, there have been some literature reports on the successful application of this technology to the detection of pathogens, but there is no research on its application to TGEV detection

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  • RT-LAMP (reverse transcription loop-mediated isothermal amplification) detection method for transmissible gastroenteritis of swine TGE
  • RT-LAMP (reverse transcription loop-mediated isothermal amplification) detection method for transmissible gastroenteritis of swine TGE
  • RT-LAMP (reverse transcription loop-mediated isothermal amplification) detection method for transmissible gastroenteritis of swine TGE

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Embodiment Construction

[0070] The RT-LAMP detection method of porcine transmissible gastroenteritis TGE described in the present invention will be further described below in conjunction with specific examples.

[0071] Unless otherwise specified, all scientific and technical terms in this application have the same meaning as commonly understood by those of ordinary skill in the art to which this invention belongs. The following examples are intended to further illustrate the specific ways of realizing the present invention, but should not be construed as limiting the present invention. Under the premise of not violating the spirit and principles of the present invention, the technical solutions obtained by modifying the invention will all fall within the scope of the claims of the present invention.

[0072] In this application, unless otherwise specified, the implementation of the present invention uses conventional techniques of molecular biology, microbiology, recombinant DNA and immunology, whic...

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Abstract

The invention provides an RT-LAMP (reverse transcription loop-mediated isothermal amplification) detection method for transmissible gastroenteritis of swine TGE. The RT-LAMP detection method comprises the following steps: 1) designing an LAMP primer by using an online software according to a conserved region of a TGEVN (TGE Virus N) gene in a GenBank; 2) preparing a virus solution for use at the temperature of 80 DEG C below zero; 3) extracting RNA (Ribose Nucleic Acid) of TGEV; 4) taking the RNA of the TGEV obtained in the step 3) as a template for RT-LAMP reaction; and 5) detecting an RT-LAMP reaction result with agarose gel. Compared with the conventional method, the RT-LAMP detection method is simple in operation, quick, and high in efficiency, specificity and sensitivity.

Description

[0001] technical field [0002] The invention is suitable for rapid and accurate diagnosis of porcine transmissible gastroenteritis in clinics and primary laboratories. [0003] Background technique [0004] Porcine transmissible gastroenteritis (TGE) is a highly contagious enteric disease caused by porcine transmissible gastroenteritis virus (TGEV). It is clinically characterized by vomiting, severe diarrhea and dehydration in pigs. Pigs of different breeds and ages can be infected, especially piglets less than two weeks old and weaned piglets are most susceptible, with a high fatality rate of up to 100%. [1] . The disease is a worldwide disease that brings huge economic losses to animal husbandry production. Early detection and diagnosis are of great significance to the prevention and treatment of this disease. To achieve early diagnosis requires rapid, sensitive and specific diagnostic methods. [0005] At present, the conventional methods for detecting TGEV mainly...

Claims

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Application Information

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IPC IPC(8): C12Q1/70C12Q1/68
CPCY02A50/30
Inventor 贺东生陈瑞爱钟望刘好鹏张显浩裴仉福唐绪汤钦
Owner GUANGDONG WENS DAHUANONG BIOTECH
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