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Triple live vaccine for swine transmissible gastroenteritis virus, swine epidemic diarrhea virus and swine rotavirus

A technology for porcine epidemic diarrhea and porcine rotavirus, which is applied in the field of triple live vaccines, can solve problems such as reinvigoration and complex virus components, and achieve the effects of avoiding trouble, good immune efficacy, and improving safety and immune efficacy.

Active Publication Date: 2013-03-06
PU LIKE BIO ENG
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] In the prior art, inactivated vaccines are often used for animal vaccines, because live viruses, such as attenuated or attenuated strains, are used in live vaccines. However, due to the problem of returning to strength in attenuated or attenuated strains in actual use, it is usually Avoid using live vaccines as immunization methods, especially multi-linked live vaccines or multi-linked live vaccines. Due to the complex composition of the virus, the chance of reinvigoration is higher. Therefore, there has been no information about porcine transmissible gastroenteritis virus, Triple live vaccine against porcine epidemic diarrhea virus and porcine rotavirus

Method used

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  • Triple live vaccine for swine transmissible gastroenteritis virus, swine epidemic diarrhea virus and swine rotavirus
  • Triple live vaccine for swine transmissible gastroenteritis virus, swine epidemic diarrhea virus and swine rotavirus
  • Triple live vaccine for swine transmissible gastroenteritis virus, swine epidemic diarrhea virus and swine rotavirus

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] Embodiment 1, the screening of suitable cell for propagating virus

[0046] Porcine TGEV was inoculated into ST cells (ATCC), PK15 cells (China Animal Disease Control Center), IBRS-2 cells (Wuhan Type Culture Collection Center) and suckling pig kidney primary cells, and porcine epidemic diarrhea virus was inoculated in Vero cells (Shanghai Institute of Biochemistry), VeroE6 cells (Shanghai Institute of Biochemistry), PK15 cells (China Animal Disease Control Center), IBRS-2 (Wuhan Type Culture Collection Center), porcine rotavirus was inoculated into Marc145 cells (China Animal Center for Disease Control), MA104 cells (Wuhan Typical Culture Collection Center), in their respective maintenance solutions, control the pH value before filtration to 7.3-7.4, and the temperature is 35 ° C to 37 ° C, continuous subculture for 8 generations, so that the virus For suitable cells, the virus proliferation titer is measured, and the cell with the highest virus proliferation titer is ...

Embodiment 2

[0059] Embodiment 2, the preparation and inspection of the triple live vaccine of porcine transmissible gastroenteritis, porcine epidemic diarrhea and porcine rotavirus

[0060] 1. Materials and methods

[0061] 1.1 cells

[0062] ST cells were purchased from American type culture collection (ATCC);

[0063] Vero E6 cells were purchased from the Cell Resource Center of Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences;

[0064] Marc145 cells were purchased from China Animal Disease Control Center;

[0065] 1.2 Preparation of poisonous seeds for production

[0066] Preparation of transmissible gastroenteritis virus virus seeds: Inject the ST cells that have grown dense monolayers into the qualified TGEV seed virus at 0.001 M.O.I (infection dose per unit cell), absorb at 37°C for 1 hour, and add a final concentration of 2.2 g / L NaHCO 3 , 10 mg / L trypsin, 1% DMSO (v / v), 100 U / ml penicillin, 100 μg / ml streptomycin, MEM maintenance solution without fetal...

Embodiment 3

[0130] Embodiment 3, the triple live vaccine of porcine transmissible gastroenteritis, porcine epidemic diarrhea and porcine rotavirus and self-made rotavirus attenuated vaccine and commercially available infectious gastroenteritis and epidemic diarrhea dual live vaccine immunization Efficacy comparison test.

[0131] 1 material

[0132] The triple live vaccine of porcine transmissible gastroenteritis, porcine epidemic diarrhea and porcine rotavirus, the vaccine product (lot number: V20100303) in the implementation example 2;

[0133] Porcine rotavirus attenuated vaccine, laboratory trial product;

[0134] Porcine transmissible gastroenteritis, porcine epidemic diarrhea dual live vaccine, Jilin Zhengye Biological Products Co., Ltd. (batch number 20100102).

[0135] 2 methods

[0136] 2.1 The preparation method of rotavirus attenuated vaccine

[0137] The preparation of the attenuated rotavirus vaccine is 10% of the prepared virus content in item 1.4 of Example 2 8 TCID 5...

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Abstract

The invention provides a triple live vaccine for a swine transmissible gastroenteritis virus, a swine epidemic diarrhea virus and a swine rotavirus and a preparation method thereof. The content of the three viruses is not less than 107.5 TCID50 (Tissue Culture Infectious Dose 50) / mL, and the volume ratio is 1:1:1. The triple live vaccine provided by the invention solves the problem that a multiple vaccine for effectively preventing and treating such three diseases as swine transmissible gastroenteritis, swine epidemic diarrhea and the swine rotavirus is not available on the current market, and especially realizes the prevention and control on the swine rotavirus. Compared with the existing method of inoculating with three simplex vaccines to prevent such three transmissible diseases, the triple live vaccine provided by the invention is economical to use, simplifies the immunization procedure and lowers the epidemic prevention cost, thereby providing a new simple and convenient immunization way for farms in China.

Description

technical field [0001] The invention relates to a multiple animal live virus vaccine, in particular to a triple live vaccine of porcine transmissible gastroenteritis virus, porcine epidemic diarrhea virus and porcine rotavirus. Background technique [0002] Porcine transmissible gastroenteritis (TGEV) is a highly contagious intestinal infectious disease of pigs. It has an acute onset and rapid spread. Pigs of all ages can be infected, causing vomiting, severe Diarrhea and high mortality (usually 100%) are the characteristics, while older or adult pigs are almost not dead, but the economic losses caused by fat loss, reduced feed remuneration, increased drugs and manpower, etc. are very serious. The disease is one of the worldwide diseases. [0003] Porcine epidemic diarrhea (PED) is an acute, highly contagious infectious disease characterized by watery diarrhea, vomiting, dehydration, and high mortality in neonatal piglets. PEDV is mainly transmitted naturally through the o...

Claims

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Application Information

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IPC IPC(8): A61K39/295A61P31/14A61K39/15A61K39/225
Inventor 张许科孙进忠白朝勇
Owner PU LIKE BIO ENG
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