38results about How to "Avoid crossbreeding" patented technology

The invention discloses a multiple liquid-phase gene chip primer, a kit and an analysis method for simultaneously detecting seven aminoglycoside drug-resistant genes; (6')-Ie-aph(2")-Ia, aph(3')-IIIa, ant(4')-Ia, ant(9)-Ia, aadE, aph(3')-Ia and aph( 3")-Ib, and has high sensitivity and high accuracy, which can realize the simultaneous detection of multiple different target molecules in the same sample. Primers, kits and analysis methods; its technical scheme includes primer nucleotide sequences such as SEQ ID NO: 1-The primer shown in SEQ ID NO: 4; the invention belongs to the field of pathogen detection of experimental animals.

The invention provides a yarn impurity removing device in a warp guide device of a warp knitting machine, and belongs to the technical field of machines. The warp guide device of the warp knitting machine comprises a machine frame and a tank arranged on the machine frame, wherein a lubricating shaft is rotationally arranged in the tank. The yarn impurity removing device comprises an air pipe arranged above the tank, and the axial direction of the air pipe is consistent with the axial direction of the lubricating shaft; the two ends of the air pipe are a closed end and an opened end respectively, a plurality of exhaust holes are formed in the lower side of the air pipe in a penetrating mode, and the exhaust holes are aligned with the lubrication shaft; a fan is further fixedly arranged on the machine frame, the fan comprises a straight tubular air outlet part, and a stainless steel corrugated pipe making the opened end and the air outlet part communicated is arranged between the openedend and the air outlet part; an adjusting pipe and a connecting column are vertically arranged on the lower portion of the closed end, and the lower end of the adjusting pipe is fixedly connected withthe machine frame; the upper end of the connecting column is fixedly connected with the closed end, the lower end of the connecting column is inserted into the adjusting pipe, and a detachable locking mechanism for fixing the connecting column and the adjusting pipe together is arranged between the connecting column and the adjusting pipe. The yarn impurity removing device has the advantage thatyarn crossing can be prevented.

The invention discloses a multiple fluoroimmunoassay primer, kit and method for rapidly distinguishing 5 avian immunosuppression pathogens. Operation is simple, a target amplified fragment is acquired through a PCR, an amplified product, fluorescence coded microspheres and streptavidin-phycoerythrin are hybridized, the MFI value is read through a detector, and viruses of different types are distinguished. The method can simultaneously detect the chicken infectious anemia virus, the chicken Marek's disease virus, the avian reticuloendotheliosis virus, the avian reovirus and the chicken infectious bursa disease virus accurately, specificity is high, sensitivity is high, and repeatability is good. Compared with a traditional detection method, the method achieves simultaneous detection of multiple different target molecules in the same sample, the use number of samples is small, operation is simple and rapid, and the detection cost can be greatly reduced.

The invention discloses a multiple fluorescence immunoassay primer, kit and method capable of fast distinguishing CAV, MDV, REV and IBDV. The multiple fluorescence immunoassay primer, kit and method has the advantages that target amplification fragments are obtained through PCR, \ amplification products, fluorescence encoded microspheres and streptavidin-phycoerythrin are hybridized, MFI values are read through a detector, and different types of pathogens are distinguished; the method can simultaneously and accurately detect chicken infectious anemia viruses, chicken Marek's disease viruses, chicken reticuloendotheliosis viruses and chicken infectious bursal disease viruses and is high in specificity, high in sensitivity and good in repeatability; compared with a traditional method, the method can simultaneously detect various molecules, with different purposes, in the same sample and is low in sample use amount, simple and fast to operate, capable of greatly lowering detecting cost, good in flexibility and capable of increasing and decreasing to-be-detected pathogen types on the basis.

The invention discloses a multiple fluoroimmunoassay method capable of quickly differentiating infectious laryngotracheitis virus (ILTV), infectious bronchitis virus (IBV), myeoplasma gallisepticum (MG) and mycoplasma synoviae (MS) and a reagent. The method is simple to operate, a target amplified fragment is obtained through polymerase chain reaction (PCR); an amplified product, fluorescence coded microspheres and streptavidin-phycoerythrin are hybridized, a mean fluorescence intensity (MFI) value is read through a detector, and pathogens of different types are differentiated. According to the method, avian infectious laryngotracheitis virus, infectious bronchitis virus, myeoplasma gallisepticum and mycoplasma synoviae can be accurately detected simultaneously, and the method is high in specificity and sensitivity and good in repeatability. Compared with a conventional detection method, the method can be used to simultaneously detect various target molecules in an identical sample, the sample usage amount is small, the method is simple and quick to operate, and the detection cost can be greatly reduced. The method is good in flexibility, and the varieties of detected pathogens can be increased or decreased as required on the basis.

The invention discloses a multi-fluorescent immunoassay method for rapidly distinguishing 6 types of poultry respiratory pathogens. The multi-fluorescent immunoassay method is simple to operate; a target amplified fragment is obtained through a PCR (Polymerase Chain Reaction); then an amplified product, fluorescence coded microspheres and streptavidin-phycoerythrin are hybridized; an MFI (Mean Fluorescence Intensity) value is read through a detector to distinguish viruses of different types. According to the method disclosed by the invention, avian influenza viruses, chicken infectious bronchitis viruses, chicken Newcastle disease viruses, chicken infectious laryngotracheitis viruses, mycoplasma gallisepticum and mycoplasma synoviae can be accurately detected at the same time; the multi-fluorescent immunoassay method has high specificity, high sensitivity and good repeatability. Compared with a traditional detection method, the method disclosed by the invention realizes simultaneous detection of a plurality of types of different target molecules in the same sample; the use amount of the sample is less; the method is simple and rapid to operate and the detection cost can be greatly reduced.

The invention discloses a multiple fluorescent immunoassay method and reagent for quick distinguishing of avian influenza virus, Newcastle disease virus, Mycoplasma gallisepticum and Mycoplasma synoviae. The method is simple to perform, a target amplification fragment is acquired through PCR (polymerase chain reaction), an amplification product, fluorescence coded microspheres and streptavidin-phycoerythrin are hybridized, and MFI (mean fluorescence intensity) value is read through a detector to distinguish different types of pathogens. The method enables accurate detection for avian influenza virus, Newcastle disease virus, Mycoplasma gallisepticum and Mycoplasma synoviae, and is high in specificity, high in sensitivity and good in repeatability; compared with traditional detection methods, the method implements simultaneous detection for various different target molecules in a same sample, fewer samples are used, the operation is simple, the operation speed is high, and detection cost can be greatly reduced; the method is good in flexibility and allows addition and deduction of the types of pathogen on such basis as required.

The invention discloses a multiple liquid phase gene chip method and a reagent for rapidly detecting guinea pig LCMV, SV, PVM and Reo-3 viruses. The operation is simple. A target amplified fragment is acquired through PCR, and then the amplified product, fluorescent coding micro-balloon and streptavidin-phycoerythrin are hybridized, MFI value is read through a tester and different viruses are distinguished. According to the method provided by the invention, the rat lymphocyte choriomeningitis virus, sendai virus, rat pneumonia virus and Reo-3 virus can be accurately detected at the same time; the specificity is strong, the sensitivity is high and the repeatability is excellent; compared with the traditional detection method, the method has the advantages that different target molecules in a same sample can be detected, the detection flux is high, the sample dosage is less, the operation is simple and quick, the detection cost is greatly reduced and the detection efficiency is increased.