52 results about "Immunologic preparation" patented technology

The invention discloses a composite IgY antibody for resisting COVID-19 and SARS. The antibody is prepared by the following method: constructing a recombinant antigen of SARS-CoV-2 and SARS-CoV; preparing an immune preparation with the recombinant antigen to immunize egg-producing birds, and obtaining the specific composite IgY antibody from the immunized eggs. A spray prepared by the antibody canbe used for broad-spectrum killing of SARS-CoV-2 and SARS-CoV, and is suitable for spraying on nasal cavity, oral cavity, skin and other parts. The antibody preparation has a specific virus neutralizing effect, can effectively control infection sources, protect susceptible people, disinfect and protect first-line medical staff and prevent spread of SARS-CoV-2.

The invention relates to a bursin extracting method and its application to curing disease and immunity, having important value in application in the aspects of heightening organismal immunity and acting as immunoenhancer, heightening effect of vaccine, etc., and able to heighten body fluid and cell immune functions of mammal at the same time. It can be used to prevent and cure infectious diseases and young animal diseases singly or together with other drugs such as antivirus and antibacterial drugs or immunomodulators, also be applied to animal vaccine as adjuvant or immunoenhancer to strengthen the disease-resistant ability and immunoresponse ability to peculiar antigens, thus heightening the immune effect.

The invention discloses a Saddletail grouper antimicrobial peptide LEAP-2 gene, vector, recombinant strain and protein, and application thereof. The amino acid sequence of the Saddletail grouper antimicrobial peptide LEAP-2 precursor protein is disclosed as SEQ ID NO.2, or the Saddletail grouper antimicrobial peptide LEAP-2 precursor protein is a protein with identical or higher activity prepared by performing substitution, deletion and / or addition on one or more of amino acids and / or terminal modification on the amino acid sequence disclosed as SEQ ID NO.2. The invention also discloses a Saddletail grouper antimicrobial peptide LEAP-2 gene sequence and a LEAP-2 mature peptide gene mLEAP-2 nucleotide sequence subjected to code modification; and thus, the Saddletail grouper antimicrobial peptide LEAP-2 gene enriches the gene bank of grouper, can be used for preparing a recombinant protein, antimicrobial preparation, fish immune preparation or feed additive, and provides new theoretical and practical basis for physiologic immunity research of grouper.

The invention relates to a multi-epitope artificial antigen of plasmodium falciparum and an application thereof. The invention designs a polynucleotide with a sequence as shown in SEQ ID No:1 and a polypeptide with a sequence as shown in SEQ ID No:2, wherein the polynucleotide and the polypeptide are used as artificial antigens to prepare vaccines, and antibodies can be obtained from the antigens and are used to prepare immune preparations against plasmodium falciparum infections and kits for detecting malaria infections. Compared with anti plasmodium falciparum vaccines in the prior art, the multi-epitope artificial antigen of the invention has high immunogenicity and antigen recognition diversity, and the antibody of the invention has high inhibition efficiency on the in vitro growth of plasmodium falciparum.

The invention provides a staphylococcus aureus enterotoxin B (SEB) immune preparation SEB2-HSP65 and its preparation method and use. The preparation method comprises the following steps of modifying a SEB gene into a SEB2 gene, fusing the SEB2 gene and a gene of a heat shock protein HSP65 to obtain a core gene segment SEB2-HSP65, and carrying out expression of the core gene segment SEB2-HSP65 to obtain a recombinant fusion protein SEB2-HSP65. The recombinant protein SEB2 does not have a TCR cell receptor binding capacity thereby solving the problem that the existing SEB immune preparation produces a large amount of inflammatory factors, and the HSP65 enhances immunogenicity of the recombinant fusion protein SEB2. The recombinant fusion protein SEB2-HSP65 as an immune preparation for animal immunization does not need any immunologic adjuvants, can produce high-titer antibodies, can help animals to resist 5*LD50SEB toxin attack and has a protection rate of 100%. The recombinant fusion protein SEB2-HSP65 has large development and use values.

A tacrolimus separation and purification method is provided. Two medium-pressure chromatographic devices are adopted. A tacrolimus fermentation extract liquid having low chromatographic purity is adopted as a raw material. Polysaccharides, protein, and other impurities are removed through a first chromatographic column; then a solvent used for extraction is removed through a rotary evaporation process; an obtained product is dissolved again; a tacrolimus-containing eluate having a low concentration is obtained by separation through a second chromatographic column; the eluates in a plurality ofbatches are combined; and the mixture is concentrated by a nanofiltration membrane to obtain high-purity solid-state tacrolimus. Through the method, direct purification from a tacrolimus-containing fermentation solution can be achieved, the tacrolimus purity is significantly increased, and medicine effects of immunologic preparations prepared from the tacrolimus are indirectly improved.

The invention discloses the usage of TolC protein. The TolC has strong serum reaction, is used to detect and diagnose dysentery, and is used as protective antigens to prepare vaccine.

The invention discloses recombinant serum amyloid A capable of enhancing immune response of crassostrea gigas. The recombinant serum amyloid A is characterized that the amino acid sequence is represented by SEQ ID NO. 1. Gene expression of cytokines CgIL17-1, CgIL17-5, and CgTNF in blood lymphocytes can be remarkably promoted inside and outside bodies of the crassostrea gigas, the immune responselevel of the crassostrea gigas can be enhanced, and the recombinant serum amyloid A has application value in preparation of novel immune preparations and other drugs for shellfishes, especially breeding shellfishes.

The invention discloses a penaeus vanmamei LvDJ-1 protein as well as a coding gene and an application thereof. The LvDJ-1 protein of the penaeus vanmamei is firstly obtained, the amino acid sequence of the LvDJ-1 protein is indicated as SEQ ID NO.2 or the LvDJ-1 protein is protein with one or more amino acid being substituted by the amino acid sequence indicated by the SEQ ID No.2, omitted and / or added and / or derived by the amino acid sequence indicated by the SEQ ID No.2 with end being modified and equal or higher activity. The experiment proves that the penaeus vanmamei LvDJ-1 protein can improve the stress resistance of the penaeus vanmamei and can be used for preparing immune preparation or feed additive related to the aquatic animals, and the application potential is wide.

The invention discloses a method for detecting bastard halibut LITAF gene expression by applying reverse transcription-polymerase chain reaction (RT-PCR). The method lays a foundation for studying bastard halibut LITAF gene expression regulation mechanism and immunology function. TNF-alpha plays an important role in the process of immune response and foreign pathogenic bacteria killing, and the LITAF is a transcription factor of TNF-alpha gene and directly controls the expression of the TNF-alpha gene. Therefore, the expression abundance of the LITAF gene reflects the activity of a bastard halibut immune system to a certain extent and can be used as an immune monitoring index and immune agent quality evaluation index in prevention and treatment of bastard halibut diseases. By detecting the variation of the bastard halibut LITAF gene expression quantity, whether the bastard halibut is infected with a disease can be judged in advance and a measure of prevention and treatment can be taken timely so as to avoid unavoidable loss resulting from serious situation; and besides, the advantages and disadvantages of a fish immune agent can be evaluated so as to judge which immune agent has better effect during prevention and treatment of flounder diseases. The invention provides a technology platform for relative quantitative analysis of LITAF gene at the mRNA level.

The invention discloses an immune preparation for treating tumor; the immune preparation contains dendritic cells (DC) loaded with a tumor antigen and cytokine-induced killer cells (CIK), the concentration of the DC loaded with the tumor antigen in a culture liquid is 1*10<7> to 1*10<8>, and the concentration of the CIK in the culture liquid is 1*10<9> to 1*10<10>. The invention further provides a preparing method of the immune preparation for treating tumor; the preparing method comprises the steps: collection of mononuclear cells of peripheral blood; isolation and culture of the DC and the CIK; extraction of a tumor antigen gene and viral vector packaging; transfection of the DC with a virus mediated tumor antigen gene; and co-culture of the DC loaded with the tumor antigen and the CIK. The accuracy of positioning of re-transported immune cells in a human body can be improved. The objective defect of a conventional cell immunotherapy technology in the same field is not good in solid tumor treatment effect can be effectively improved; adaptation diseases are wide, and the use is safe and effective.

The invention relates to the field of animal immune preparations, in particular to an adenitis equorum disease inactivation vaccine and a preparation method thereof. The adenitis equorum disease inactivation vaccine is prepared by taking a high-generation culture strain ES14-09 of an adenitis equorum streptococ cus Erdos epidemic strain ES14 as specific immunizing antigen through processes of seed batch preparation, culture, concentration, inactivation and adjuvant emulsification. The content of antigen bacteria of the adenitis equorum disease inactivation vaccine is 5*10<8> / ml. The adenitis equorum disease inactivation vaccine is high in protection rate and applicable to epidemic prevention of adenitis equorum diseases of equine animals.

The present invention relates to an anti-infection and anti-tumor mucosal immune preparation. The mucosal immune preparation includes mucosal immune substances that are mainly formed by organically bonding polyinosinic-polycytidylic acid, non-antibiotic amino compounds and metal cations through chemical bonds. The present invention provides a slow release effect on a local part or the whole body, prevents the degradation of serum ribonucleases of human beings and primates, prolong the half-life period of the mucosal immune preparation, increases the availability and effectiveness of drugs. The mucosal immune preparation can facilitate the mucosal immunity of the body by mucosal immunity and thus facilitate the activation and proliferation of various immune cells, rather than merely acting on diseased local parts, so that the purposes of anti-infection and anti-tumor prevention and treatment with almost no side effects are realized. Mucosal immunity also avoids the pain of repeated injection so that good compliance is achieved.

The invention relates to a bidirectional oil emulsion agent used in immunity agent, which comprises animal injection white oil, Siban 80, tuwen 80, stabilizer 1.2 propanediol, activator PVP polyvinyl pyrrolidon. And the production comprises that in room temperature, first emulsifying animal injection white oil in an emulsifying pot, to obtain light liquid wax oil, to be added with the lipophilic SIban-80, to be mixed for 3-7min at 75-80r / min speed until foam disappears and transparent, then mixing and adding hydrophilic tuwen-80 to be mixed for 3-7min uniformly, adding 1.2 propanediol, mixing uniformly, adding polyvinyl pyrrolidon until foam disappears and transparent, laying for 25-35min. The invention has low viscidity, injection support, uniform size, and adsorption support, with high stability, long service life, and the color in white or milky white with some pink.

The invention relates to the technical field of bioactive peptides and provides application of a tilapia protein peptide to preparation of an immune preparation. The tilapia protein peptide refers toenzymolysis polypeptide obtained by enzymolysis of tilapia protein. According to researches, the tilapia protein peptide is capable of effectively inducing RAW264.7 cell iNOS expression and increasingNO release amount, and due to dose dependence, the tilapia protein peptide is excellent in immunoregulatory activity and can be used as a raw material for the immune preparation. Further, according to the researches, the tilapia protein peptide has no evident influences on RAW264.7 cell activity under immunoregulatory dosage, namely the tilapia protein peptide has no cytotoxicity when giving playto an immunoregulatory effect. The tilapia protein peptide is preferably derived from enzymatic hydrolysates of tilapia leftovers, and a novel application approach is provided for recycling of tilapia byproducts.

The present invention provides a biological product capable of resisting stenotrophomonas maltophilia and Pseudomonas aeruginosa infection and raising the body fluid immunity and cell immunity of malignant tumor patient. The biological product is prepared with stenotrophomonas maltophilia, and may be prepared into various kinds of immunological preparations and immune regulating preparations. The medicine of the present invention has the special effect of preventing and treating stenotrophomonas maltophilia and Pseudomonas aeruginosa infection and treating ascites carcinoma and leukemia.

The invention refers to a nucleic acid compound, the producing method and the application at the aspect of making immune preparation, produced by making copolymerization or covalence reaction on nucleic acid segment, multi-peptide and water-solubility multi-polymer under catalysis. It has the common formula; nucleic acid / multi-peptide / water-solubility multi-polymer. It can protect the organism to resist the pathogene infection, resist the tumour and cure the independent immune diseases and effectively inspires the immune response of organism.

A bioimmunological medicine for treating the mastitis of milk cow contains hog lymphocyte transfer factor and ox lymphocyte transfer factor. It is prepared from the spleens of hog and ox through mincing, proportionally mixing, mashing, homogenizing, repeated freezing-thawing, freezing, centrifugating, taking supernatant, dialysis, freezing, thawing and filtering.

The invention provides an immunological preparation for preventing and treating porcine reproductive and respiratory syndromes. The immunological preparation comprises epimedium, sealwort, Gorgon fruits, ephedra herbs, centipeda minima, flatstem milkvetch seeds, forsythia, companumoea roots, cape jasmines, yams, tree peony bark, acanthopanax roots, curculigo orchioides, jasminum elongatum, clovers, meadowrue roots and rhizomes, mistletoes, rheum franzenbachii, adenophora tetraphylla and alpinia oxyphylla. The immunological preparation has the advantages that cellular immunity and humoral immunity of organisms can be enhanced through the organism regulation effect, immune cells are activated, interferons are induced, viruses cannot survive and reproduce in porcine body cells, and the effect of resisting the porcine reproductive and respiratory syndromes is achieved.

The invention relates to a biological immune preparation for strengthening a goatpox vaccine antibody and a manufacturing method of the biological immune preparation. The steps adopted in the method include that the fresh spleen of a healthy yak is selected, after fat, the envelope and the internal connective tissue are removed, the spleen is subjected to freezing preservation, the yak spleen obtained after freezing preservation is taken out and melted and is minced through a tissue stamp mill, after sterile tri-distilled water with the same weight is added, the high-speed tissue stamp mill is used for conducting homogenizing at a high speed for two times, and homogenizing is conducted 10 min each time; and prepared homogenate is frozen at the temperature of minus 20 DEG C, freezing and thawing are conducted repeatedly for five times, centrifuging is conducted after the last time of thawing, supernatant is taken and put into a dialysis bag, the supernatant is dialyzed overnight under the environment of 0 DEG C to 4 DEG C through pyrogen-free cold normal saline, dialysate outside the bag is collected and is subjected to filtration sterilization through a filtering film, and the dialysate is subjected to sterile subpackaging and is then subjected to freezing preservation. After the biological immune preparation enters an organism, the effect of the biological immune preparation can be rapidly developed and can last for several months or even longer, the immune antibody level of the vaccine is effectively improved, the biological immune preparation can be rapidly and completely absorbed by the organism without any residue, weight increasing is obvious after the biological immune preparation is injected into the organism, and a goat can be lively in spirit.

The invention discloses an application of an immunostimulating compound in preparing fish immune preparation, and the immune preparation is an oral immune preparation or a bath immune preparation. The advantage of the present invention is that: the oral immune preparation or bath immune preparation made of the immune stimulating compound ISCOMs can not only increase the antibody level, but also improve the proliferation and transformation ability of T lymphocytes and induce immune protection. When immunized with the same dose, the antibody titer of ISCOMs group was significantly higher than that of no adjuvant group and ISM1312 adjuvant group. Similarly, oral immunization with ISCOMs can not only increase the antibody level, but also improve the proliferation and transformation ability of T lymphocytes and induce immune protection.

The invention discloses an external medicine capable of resisting HPV infection and converting HPV positive to HPV negative and a preparation method thereof. The external medicine is prepared from the following raw materials in parts by weight: 20 to 50 parts of hedgehog fat, 10 to 30 parts of frankincense, 10 to 20 parts of radix angelicae, 10 to 20 parts of radix ranunculi ternati, 5 to 15 parts of radix et rhizoma rhei, 5 to 10 parts of airpotato yam rhizome, 5 to 10 parts of dried alum and 2 to 5 parts of realgar. Years of clinical experience prove that the medicine disclosed by the invention has no adverse reaction, the medicine can avoid the first-pass effect of the liver when being externally used, has a targeting effect, and has a higher inhibition rate on host cytopathy caused by HPV than that of immune preparations and interferon.

The invention discloses a livestock biological immune preparation and a preparation method thereof. The effective component of the immune preparation is a specific yolk immunoglobulin composite antibody. According to the preparation method of the specific yolk immunoglobulin composite antibody, the specific yolk immunoglobulin composite antibody is prepared by taking three pathogenic bacteria k88, k99 and 987P of swine escherichia coli as antigens, and has a relatively good killing effect on three common pathogenic escherichia coli with high morbidity and high mortality in intestinal tracts. The traditional Chinese medicine composition can well regulate the balance of microbial flora in intestinal tracts, has a good prevention and treatment effect on livestock diarrhea, reduces the death rate of livestock, and is suitable for large-scale production and clinical application.

The invention discloses a bacillus anthracis capable of showing a PA20 protein on the surface of a spore and an application thereof. The bacillus anthracis is obtained by inserting a pagA20 gene into the tail end of a bacillus anthracis spore protein bcIA gene; and particularly the bacillus anthracis can be a bacillus anthracis CGMCC No.3403. The bacillus anthracis can serve as a host and is used for preparing, an immunomodulator which is preferably an inactivated vaccine, an attenuated live vaccine, a subunit vaccine or a gene engineering vaccine.

A bioimmunological medicine for treating the mastitis of milk cow contains hog lymphocyte transfer factor and ox lymphocyte transfer factor. It is prepared from the spleens of hog and ox through mincing, proportionally mixing, mashing, homogenizing, repeated freezing-thawing, freezing, centrifugating, taking supernatant, dialysis, freezing, thawing and filtering.

The invention discloses a Hefang crucian(WR) Ferritin L gene and a recombinant protein thereof. The nucleotide sequence of the WR Ferritin L gene is shown as SEQ ID NO: 1, and the amino acid sequenceof the recombinant protein is shown as SEQ ID NO: 2. The discovery of the WR ferritin L gene enriches a gene pool of WR and can be applied to preparation of the recombinant protein. Experiments provethat the recombinant protein can inhibit the growth activity of aeromonas hydrophila, and a new practical basis is provided for physiological immune research of the WR. The invention further disclosesa preparation method of the WR ferritin L recombinant protein. The invention discloses application of the WR Ferritin L recombinant protein in the fields of inhibition of growth of aeromonas hydrophila, immune preparations for aquatic animals or feed additives, and discloses primers for amplifying the WR Ferritin L gene.

The invention relates to a preparation method and application of an immune preparation capable of enhancing immunities of fishes, prawns and crabs, and belongs to the technical field of nutrition immunology. By feeding an immuno-potentiator containing such nutrients as nitric oxide, amino acid, beta-brown shell color, anthocyanin, vitamins and trace elements, oxygen radicals and viruses in the fishes, prawns and crabs are removed or inhibited; moreover, the immune preparation can regulate metabolism of bred animals, and has a certain growth promoting effect; the immune preparation is favorable for reducing a death rate and improving breed output of the bred animals.