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Penaeus vanmamei LvDJ-1 protein as well as coding gene and application thereof

A white penaeus, lvdj-1 technology, applied in the field of genetic engineering, can solve the problems of high morbidity and mortality, pollution, economic impact on artificial breeding, etc., to improve anti-stress ability, broad application potential, high The effect of economic value

Active Publication Date: 2014-10-01
SOUTH CHINA NORMAL UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, due to the current rapid climate change disasters and severely polluted water quality problems, the morbidity and mortality rates remain high, causing serious economic blows to the artificial culture of Penaeus vannamei. solved problem

Method used

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  • Penaeus vanmamei LvDJ-1 protein as well as coding gene and application thereof
  • Penaeus vanmamei LvDJ-1 protein as well as coding gene and application thereof
  • Penaeus vanmamei LvDJ-1 protein as well as coding gene and application thereof

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Embodiment Construction

[0030] The present invention will be further described below in conjunction with the examples, but not limited thereto.

[0031] The molecular biology experimental techniques used in the following examples include PCR amplification, plasmid extraction, plasmid transformation, DNA fragment ligation, enzyme digestion, gel electrophoresis, etc., unless otherwise specified, generally operate according to conventional methods. For details, please refer to " Molecular Cloning Experiment Guide (Third Edition) (Sambrook J, Russell DW, Janssen K, Argentine J. Huang Peitang et al. translation, 2002, Beijing: Science Press), or according to the conditions suggested by the manufacturer.

[0032] 1. Obtaining the ORF of the LvDJ-1 gene of Penaeus vannamei

[0033] According to the EST splicing in the cDNA library at http: / / www.ncbi.nlm.nih.gov / , the sequence of LvDJ-1 including ORF was obtained. The first primer is LvDJ-1S1, [5'-TACACAGGCCTTCTCCTCCCAAC] (SEQ ID NO.3), the second primer ...

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Abstract

The invention discloses a penaeus vanmamei LvDJ-1 protein as well as a coding gene and an application thereof. The LvDJ-1 protein of the penaeus vanmamei is firstly obtained, the amino acid sequence of the LvDJ-1 protein is indicated as SEQ ID NO.2 or the LvDJ-1 protein is protein with one or more amino acid being substituted by the amino acid sequence indicated by the SEQ ID No.2, omitted and / or added and / or derived by the amino acid sequence indicated by the SEQ ID No.2 with end being modified and equal or higher activity. The experiment proves that the penaeus vanmamei LvDJ-1 protein can improve the stress resistance of the penaeus vanmamei and can be used for preparing immune preparation or feed additive related to the aquatic animals, and the application potential is wide.

Description

technical field [0001] The invention belongs to the technical field of genetic engineering, and in particular relates to the LvDJ-1 protein of Penaeus vannamei and a gene encoding the protein, a carrier containing the gene and an application of the protein. Background technique [0002] In 1997, Nagakubo et al. used yeast two-hybrid technology to screen out a new protein that interacts with c-myc, and discovered DJ-1 for the first time, which is a common pathogenic gene of Parkinson's disease (PD). In the form of an asymmetric dimer, it participates in various life activities of cells, such as nerve injury, tumorigenesis, transcription regulation, molecular chaperone, immunity, and anti-oxidation. The monomer of DJ-1 protein is composed of 11 sheets and 8 helices. Among them, β3 and β4 folds form a β-hairpin structure and participate in the dimerization of DJ-1 protein. The specific mechanism by which DJ-1 participates in various life activities is still unclear. Vibrio al...

Claims

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Application Information

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IPC IPC(8): C07K14/435C12N15/12C12N15/81A23K1/16A23K1/18C12R1/84A23K20/147A23K50/80
CPCA23K20/147A23K50/80C07K14/43509
Inventor 王维娜黄明珠刘媛
Owner SOUTH CHINA NORMAL UNIVERSITY
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