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Biological immune preparation for treating dairy cattle clinical mamitis and its manufacturing method

A technology of immune preparations and production methods, which is applied in the directions of anti-inflammatory agents, non-central analgesics, medical preparations containing active ingredients, etc. Improve immune function, avoid slow action, improve immune function

Inactive Publication Date: 2005-03-09
NORTHWEST A & F UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Disadvantages of antibiotic use: due to long-term use of antibiotics, pathogenic bacteria in dairy cows have developed drug resistance
[0006] Disadvantages of mastitis vaccine: At present, some companies or individuals at home and abroad isolate pathogenic bacteria from mastitis-affected cows, culture and inactivate them to make inactivated vaccines, and then immunize cows to prevent the occurrence of cow mastitis
But its therapeutic effect is not as good as that of antibiotics, and its curative effect is slow and the formula is complicated
More importantly, when the preparation is used, it needs to be fed, which consumes a lot of manpower
If it is added in the feed, it will affect the therapeutic effect, or the palatability is poor, which will affect the feed intake of dairy cows

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Example 1: Porcine lymphocyte transfer factor 18iu

[0025] bovine lymphocyte transfer factor 2iu

Embodiment 2

[0026] Example 2: Porcine lymphocyte transfer factor 10iu

[0027] Bovine lymphocyte transfer factor 10iu

[0028] crafting process:

[0029] Fresh spleens of healthy pigs and cattle were selected from the slaughtering workshop, and after inspection of the spleen for no lesions and normal color, the disassociated tissue on the surface and inside of the spleen was removed, and then frozen. Take the above-mentioned frozen spleen, and mince it after it is thawed. Mix the minced pig spleen and beef spleen, each portion according to pig spleen: beef spleen = 0 ~ 10: 0 ~ 10 (weight ratio), then add sterile three-distilled water equal to the weight of the spleen, and then mash the tissue Slurry, the speed is about 8000~10000 rpm, and the time is about 6~10 minutes. The process of mashing and homogenizing the tissue should be carried out intermittently, that is, it is better to stop the homogenization for about 10 minutes every 3 minutes. The purpose is to prevent the ho...

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Abstract

A bioimmunological medicine for treating the mastitis of milk cow contains hog lymphocyte transfer factor and ox lymphocyte transfer factor. It is prepared from the spleens of hog and ox through mincing, proportionally mixing, mashing, homogenizing, repeated freezing-thawing, freezing, centrifugating, taking supernatant, dialysis, freezing, thawing and filtering.

Description

1. Technical field: [0001] The invention relates to a biological immune preparation, in particular to a biological immune preparation for treating cow clinical mastitis and a preparation method thereof. 2. Background technology: [0002] Clinical mastitis of dairy cows has become one of the most serious and common diseases in dairy farms, with an incidence rate as high as 30%-80%. It seriously affects the milk production of dairy cows and reduces the economic benefits of dairy farms. According to expert estimates, the global milk loss caused by cow mastitis can reach 3.8 million tons every year. More importantly, the quality of milk produced by cows suffering from mastitis has declined, and the content of harmful microorganisms exceeds the standard, which seriously endangers human health. Cow mastitis has become one of the key factors restricting the healthy and rapid development of dairy farming industry. [0003] Cow mastitis is caused by the infection of cow udders by a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K35/26A61K38/02A61P15/14A61P29/00
Inventor 陈德坤
Owner NORTHWEST A & F UNIV
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