59results about How to "Growth inhibitory activity" patented technology

The invention relates to artemisinin derivatives shown as a general formula I, and optical isomers and pharmaceutically acceptable salts thereof, wherein substituents Z, Ar, R and n are defined in the specification. The invention also relates to application of the compounds with the general formula I in preparing medicaments for treating and / or preventing cancers.

The invention relates to a benzimidazole zinc complex and a preparation method thereof. A chemical name of the benzimidazole zinc complex is dichloro-2-(2-(1H-benzimidazole-2-)benzyl)-1H-benzimidazole zinc complex. The preparation method includes following steps: mixing 2-(carboxymethyl)benzoic acid with o-phenylenediamine, placing the mixture in phosphoric acid, performing a heating process at 180 DEG C for 3 hours to obtain a purple-green reactant, cooling the reactant to room temperature, adding distilled water, performing a neutralization reaction to obtain a yellow-green solid, performing a washing process through distilled water for three times, carrying out a filtering process, carrying out a recrystallization process through alcohol to obtain a yellow-green solid 2-(2-(1H-benzimidazole-2-)benzyl)-1H-benzimidazole, namely a ligand, dissolving ZnCl2 and the ligand 2-(2-(1H-benzimidazole-2-)benzyl)-1H-benzimidazole in a proper amount of a DMF solvent to obtain a mixture, stirring the mixture uniformly at room temperature, performing a volatilization process for seven days to obtain a slight yellow-green block-shaped complex monocrystal. The complex employs biological non-toxic element zinc as a metal ion, can be used for preparing antitumor drugs, can inhibit generation of esophagus cancer cells, and is free from remaining in human body and causing poisoning in human body due to metal accumulation. The complex is simple in the preparation method, is high in yield, is low in cost and is suitable for an industrial production.

The invention relates to a chonglou saponin I, namely, (25R)-Delta5(6)-alkene-spirost-3-O-{alpha-L- arabinofuranosyl (1->4)}-[alpha-L-rhamnopyranosyl (1->2)]-3-O-beta-D-glucopyranoside preparation method and an application for inhibiting angiogenesis thereof; the preparation method is composed of extracting polyphylla by using organic solvent and preparing chonglou saponin I by silica gel and reverse phase silica gel chromatography which can be used as an anti-neoplastic drug.

The invention relates to a new structure of bilactone dimer and the producing method, and the natural product selecting to human HL-60, CNE, KB, A549, BEL-7404 and Hela. It could be used to produce anti-tumor medicine.

The invention relates to artemisinin derivates as shown in a general formula I, optically active isomers and pharmaceutically acceptable salts thereof, wherein the substituents R1, R2, X and Ar have the meanings defined in the specification. The invention also relates to application of the compound shown in general formula I in the preparation of medicaments for treating and / or preventing cancer.

The invention discloses a method and a device for cultivating high-concentration photosynthetic bacteria with low illumination. The device comprises an anode chamber and a cathode chamber, wherein the anode chamber is made from a transparent material, and is separated from the cathode chamber through an ion exchange membrane; the anode chamber and the cathode chamber are respectively provided with an anode and a cathode; the anode and the cathode are connected through an external resistor; the ratio of surface area of the anode to effective capacity of the anode chamber is more than 0.06 / cm, and ratio of resistance of the external resistor to effective capacity of the anode chamber is 4-40 ohm / cm3; the anode chamber of the device is a fermentation tank; photosynthetic bacteria cultivated to a logarithmic phase are inoculated to the anode chamber at a volume percentage of 1-10%, and the anode chamber is sealed and is communicated to an external circuit to cultivate the bacteria at 20-37 DEG C with illumination of 50-500lux. The method disclosed by the invention can cultivate high-concentration photosynthetic bacteria under low inoculum size and low illumination, and can generate electric energy while reducing illumination energy consumption; and the method is wide in application prospect.

The invention provides a method for short-cut nitrification and denitrification in a constructed wetland. The method comprises the steps of setting a constructed wetland system provided with a pool I and a pool II; filling the lower layer and upper layer of the pool I with limestone; evenly mixing activated sludge and sand, and then filling the middle layer of the pool I with the mixture, so that a limestone-sand-limestone grading composition type constructed wetland system is formed; filling the pool II in the same way; adjusting the content of organic carbon according to the concentration of NH4+-N in water input into the system, so that inflow water with the C / N ratio being (4:1)-(8:1) is formed; conducting aeration on the water storage pools to enable the concentration of dissolved oxygen in water to reach 9 mg / L and hydraulic retention time to be 2.5 days. According to the technical scheme, by controlling dissolved oxygen in the constructed wetland and alkalinity of the constructed wetland, short-cut nitrification and denitrification are achieved easily and conveniently in the constructed wetland, and the removal rate of total nitrogen in sewage is increased.

The invention relates to a natural product sparganiaceae lactone B derivative as well as a preparation method and use for preparing anti-tumor drugs. The structural formula of the derivative is show in the description, wherein R is hydrogen, C1-6 alkyl, C3-6 cycloalkyl, C5-10 aryl, pyridyl, CnH2nX, OR1 or NR2R3; n is 1-6, X is vinyl, acetenyl, C5-10 aryl, azo-aryl containing 1-4 nitrogen atoms and 1-5 carbon atoms, hydroxyl, C1-4 alkoxyl, amino, N, N-biC1-4 alkyl amino, carboxyl, acylamino, sulphonylamino, C1-4 alkoxycarbonyl, cyano or nitryl; R1 is hydrogen, C1-4 alkyl, benzyl or methoxyl benzyl; and R2 and R3 are respectively hydrogen, C1-4 alkyl, benzyl, methoxyl benzyl or R2 and R3 jointly form a 4-7 membered ring with N atoms. The sparganiaceae lactone B derivative provided by the invention is used for anti-tumor drugs, and the compound provided by the invention is novel in structure, has relatively good druggability and relatively high activity of inhibiting tumor cell growth, and is simple and convenient in preparation method and easy to operate and amplify.

The invention relates to triazole derivatives of rapamycin and application. Concretely, the triazole derivatives of rapamycin comprises compounds of the following formula I, or pharmaceutically acceptable salts, solvates, isomers, esters and prodrugs thereof. In the formula I, n is 1, 2 or 3, X is carbonyl or chemical bonds, R is hydrogen or C1-C4 alkyl, R1 is hydrogen, hydroxyl, hydroxymethyl, halogens, trifluoromethyl, trifluoromethoxyl, amino, carboxyl, cyan, N-(C1-C4)alkylamino, N,N-di(C1-C4)alkylamino, (C1-C4)alkylthio, (C1-C4)alkylsulfinyl, (C1-C4)alkoxylethyl, (C1-C4)alkylacyl, carbamoyl, N-(C1-C4)alkylcarbamoyl, N,N-di(C1-C4)alkylcarbamoyl or (C1-C3)alkylenedioxyl. The compounds have excellent biological activity.

The invention discloses an epinephelus coioides antimicrobial peptide. The antimicrobial peptide is epinephelus coioides CD59 protein, and has an amino acid sequence as shown in SEQ ID NO.1, or protein having an amino acid sequence as shown in SEQ ID NO.1, which is substituted, lost and / or added with one or more amino acids and / or is terminal modified and has the same or higher activity. Experiments prove that the epinephelus coioides antimicrobial peptide can be used for inhibiting the growth activity of vibrio alginolyticus, can be applied to preparation of immune stimulant or feed additivesfor fish, especially groupers, and has a wide application prospect. The CD59 gene sequence of the epinephelus coioides is obtained for the first time, can be used for enriching the gene pool of groupers, can be applied to preparation of recombinant protein, antibacterial and bacteriostatic additive and fish immune simulants, and provides new practice foundation for physiological immune researchesof epinephelus coioides.

This invention relates to Ailinofen bi lactone and its medical used salt or solvate. This invention also relates its preparation method and its medical combination and medical application. The compound in this invention can in vitro prevent two kinds of human body tumor cells activity as oral epithelium cancer cell (KB) and human hepatic carcinoma cell (BEL-7404). It can be developed as anti tumor medicine application.

This invention relates to Ailinofen bi lactone and its medical used salt or solvate. This invention also relates its preparation method and its medical combination and medical application. The compound in this invention can in vitro prevent six kinds of human body tumor cells activity as human original medullary cell leukemia cell(HL-60), nasopharyngeal carcinoma cell(CNE), oral epithelium cancer cell(KB), human lung cancer cell(549), human hepatic carcinoma cell(BEL-7404) and human uterus neck cancer cell(Hela). It can be developed as anti tumor medicine application.

Disclosed is a human monoclonal antibody, wherein said antibody comprises: a heavy chain CDR1 region comprising amino acid sequence SEQ ID NO: 296; a heavy chain CDR2 region comprising amino acid sequence SEQ ID NO: 297; a heavy chain CDR3 region comprising amino acid sequence SEQ ID NO: 298; a light chain CDR1 region comprising amino acid sequence SEQ ID NO: 299; a light chain CDR2 region comprising amino acid sequence SEQ ID NO: 300; a light chain CDR3 region comprising amino acid sequence SEQ ID NO: 301; characterized in that said antibody has opsonic phagocytic killing activity against at least one strain of each of at least two different Enterococcus species and against at least one strain of Staphylococcus aureus.

1-aromatic aldoxime uracil and a preparation method of same. The invention relates to the field of medicines and particularly provides 1-aromatic aldoxime uracil, which can inhibit growth activity of tumor cells and significantly inhibit thymidine phosphorylase and has antitumor activity, as well as a preparation method of same. The 1-aromatic aldoxime uracil is represented by the structure (I), wherein R1 is a methyl group or hydrogen, R2 is chlorine, iodine, a carboxyl group or hydrogen, Ar is an alkyl phenyl group, halogenated phenyl group, an alkoxyphenyl group, a thienyl group or a furyl group. The 1-aryl aldoxime uracil has high activity of inhibiting growth of tumor cells, has significant inhibition effect on thymidine phosphorylase and has antitumor activity. The method has simple processes and high availability, has convenience in large-scale production and can reduce production cost.

The invention relates to indolone-containing 4-thiazolidone derivatives shown in a general formula I as well as geometrical isomers and pharmaceutically acceptable salts of the indolone-containing 4-thiazolidone derivatives. In the formula I, substituted groups namely Ar, R2, R3, R4 and n are defined in the specification. The invention also relates to application of compounds of the general formula I in preparation of a medicine for treating and / or preventing cancer.

The invention discloses a conjugated dienes derivative and a preparation method and application of the conjugated dienes derivative as an anti-cancer drug. The structure of the conjugated dienes derivative is shown as formula I. Practice shows that a compound has obvious activity on inhibiting the growth of cancer cells; and the antitumor activity of some optimized compounds is obviously superior toprior to that of a contrast drug. The preparation of such compound is simple and easy to carry out; raw materials are easy to obtainily available; and the conjugated dienes derivative is a substance with antitumor activity and has a wide application prospect.

The invention, belonging to the technical field of medicine, relates to a compound, that is, 10-hydroxyamptothecin derivative, and its preparation method and pharmaceutical application. The compound is represented as formula 1, wherein X=-H, -R, -COR, R represents alkyl with 1-8 carbon atoms, the alkyl represents alkane, alkylene or aryl; Y=-H, -R', -COR, R' represents alkyl with 1-8 carbon atoms, and the alkyl represents alkane, alkylene or aryl. The results of anti-tumor bioactivity in vitro selection tests show that the compound disclosed herein has significant inhibiting effect on the growth activity of tumor cells, and the compound can be further used for preparing anti-tumor active medicines for treating tumors.

The invention relates to a low-temperature low-ammonia-nitrogen sewage denitrification device based on a magnetocaloric effect coupling carrier. The low-temperature low-ammonia-nitrogen sewage denitrification device comprises a sewage treatment container, the magnetocaloric carrier in the sewage treatment container and a magnetic field generation device outside the sewage treatment container, wherein the magnetocaloric carrier is nano Fe3O4-loaded zeolite, and anaerobic ammonia oxidizing bacteria and nitrite bacteria grow in the magnetocaloric carrier and on the surface of the magnetocaloric carrier. According to the device, a one-stage Canon process is adopted, precise local controllable temperature rise can be realized through a magnetocaloric effect generated by an external medium-high frequency alternating magnetic field and zeolite-loaded nano Fe3O4, zeolite selectively adsorbs ammonia nitrogen to create a local high-nitrogen-carbon-ratio environment, and the device has targeted advantages for solving the problem of low-temperature low-ammonia-nitrogen sewage denitrification treatment, anaerobic ammonia oxidizing bacteria and nitrite bacteria are efficiently screened, the competitive advantages of the bacteria are kept, the microbial activity is efficiently improved with low consumption, and the capital construction and operation cost for sewage treatment is greatly reduced.

The invention provides a method for extracting and separating ganoderma triterpene extract by using a subcritical technology. The method comprises the following steps of: sequentially extracting and separating active ingredients, namely a ganoderma triterpene acid part and a ganoderma triterpene neutral part, in ganoderma from ganoderma sporocarp by using the subcritical water extraction technology under different pressure and temperature conditions. According to the method, different active extracts in ganoderma are efficiently and economically obtained by using the subcritical water extraction technology. The antioxidant activity of the DPPH clearance rate of the ganoderma triterpene acid part in the obtained active extract can reach 88.38%, the semi-inhibition ratio IC50 of the ganoderma triterpene neutral part to human large cell lung cancer cells H460 is 95.0 micrograms / ml, and the semi-inhibition ratio IC50 of the ganoderma triterpene neutral part to human hepatoma carcinoma cells HepG2 is 77.7 micrograms / ml.

The invention discloses a microbial fertilizer with an anti-continuous cropping effect. The microbial fertilizer is prepared from the following components: 70-81% of an anti-continuous cropping compound bacteria solution, 4.5-8% of a chitosan hydrolysate, 4.5-6.5% of mineral source fulvic acid / mineral source potassium fulvate and 10-15.5% of wood vinegar, wherein the anti-continuous cropping compound bacteria solution is composed of a bacillus amyloliquefaciens bacteria liquid (1), a bacillus licheniformis bacteria liquid (2) and a brevibacillus laterosporus bacteria liquid (3), and the weight ratio of the bacillus amyloliquefaciens bacteria liquid (1) to the bacillus licheniformis bacteria liquid (2) to the brevibacillus laterosporus bacteria liquid (3) is (3-5):(1-2):(0.3-0.5). The preparation method comprises the step of mixing the components. According to the application of the microbial fertilizer to resistance of pepper and cucumber continuous cropping, when the microbial fertilizer is used for resistance of pepper continuous cropping, growth activity of root-knot nematode can be inhibited, new roots can be quickly promoted, absorption of roots to moisture and nutrients can be promoted, synthesis of chlorophyll can be promoted, and yellowing can be improved; and when the compound is used for resisting cucumber continuous cropping, the morbidity of cucumber fusarium wilt can be reduced, the cucumber yield is increased, the activities of soil sucrase, phosphatase, catalase and urease are improved, and the effect is remarkable.

The invention relates to a drug use of diacetyl-diphenyl-heptane to prevent and cure chronic Bone marrow-derived leukemia. Specifically, the invention relates to the drug use of 3, 5-diacetyl group-1-(3- methoxyl group-4, 5-dihydroxy phenyl)-7-(4-hydroxy-3- methoxyl group phenyl) heptane and the medicinal salt thereof in the preparation of prevention and treatment of chronic Bone marrow-derived leukemia. The compound is extracted mainly from ginger, curcuma wenyujin, zedoary and other zingiberaceae plants. Pharmacological experiments are proved that the compound has potent inhibitory effect on chronic Bone marrow-derived leukemia cell strain (K562) of human and the adriamycin-resistant strain (K562 / ADR) proliferatione in vitro thereof, wherein, the value of IC50 is 34.9 plus or minus 0.6 and 50.6 plus or minus 23.5 Mum respectively.

The invention provides a preparation method and application of bean curd residues, and relates to the technical field of food processing. The preparation method comprises the following steps of takingfresh bean curd residues of which the water content is 60-90wt%, pouring the taken bean curds residues in water, performing uniform mixing, after mixing, performing grinding with a grinder, screeninga mixture of the ground fresh bean curd residues and water with a sieve of 30-80 meshes, and performing pressing and dehydrating treatment on the mixture of the fresh bean curd residues and the water, after being screened, so that the water content of the dehydrated bean curd residues is 50-75wt%, pouring the dehydrated fresh bean curds residues in a clean frying pan, besides, adding sugar and edible salt to a frying pan, performing heating for frying, and adding edible oil so as to obtain the bean curd residues. In the stir-frying process, the sugar can thoroughly remove moisture in the fresh bean curd residues through a penetration principle, and in the stir-frying process, powdered sugar can uniformly pack the surface of the bean curd residues, so as to further reduce water activity AWof the bean curd residues to 0-0.85. Biscuits made from the bean curd residues are good in flavor and long in quality guarantee period.

The invention relates to a biological methanation system for coupling renewable energy hydrogen production with power plant carbon dioxide capture. The system comprises a renewable energy hydrogen production subsystem used for receiving renewable energy power generation and electrolyzing water to obtain oxygen and hydrogen serving as a substrate gas; a carbon dioxide trapping subsystem which is used for trapping and recovering carbon dioxide serving as substrate gas by taking the flue gas of the power plant as a source; and a H2 / CO2 biological methanation subsystem which is used for generating methane through the metabolism of methanogens by taking hydrogen and carbon dioxide as substrate gases and merging the methane into a natural gas pipeline network. Compared with the prior art, the system has the advantages of better methane reaction effect, higher carbon dioxide conversion efficiency, smaller energy loss, high efficiency, reliability, modular construction, high expansibility, great popularization and application potential and the like. A novel green energy recycling system is constructed by applying various green low-carbon technologies. Thus, deep consumption of renewable energy sources and reduction treatment of carbon dioxide are realized.

The invention provides a method for extracting and separating Ganoderma lucidum triterpenoid extract by using subcritical technology: subjecting Ganoderma lucidum to subcritical water extraction technology, using different pressure and temperature conditions, and sequentially extracting the active components in Ganoderma lucidum: the acidic part of Ganoderma lucidum triterpene and the Ganoderma lucidum triterpenoid neutral parts were extracted and isolated from Ganoderma lucidum fruiting bodies. The invention utilizes subcritical water extraction technology to obtain different active extracts in Ganoderma lucidum efficiently and economically. The antioxidant activity of Ganoderma lucidum triterpenoid acid site DPPH clearance rate in the obtained active extract can reach 88.38%, and the half-inhibition rate IC of Ganoderma lucidum triterpenoid neutral site on human large cell lung cancer cell H460 50 95.0μg / ml, the half-inhibition rate IC of human hepatoma cell HepG2 50 was 77.7 μg / ml.

The invention discloses a microbial fertilizer with anti-stubble stubble effect, which is composed of the following components: 70%-81% of an anti-stubble-stubble compound bacterial solution, 4.5%-8% of chitosan hydrolyzed liquid, mineral source fulvic acid / mineral source fulvic acid Potassium acid 4.5%~6.5%, wood vinegar liquid 10%~15.5%; the anti-stubble compound bacteria liquid is composed of ① Bacillus amyloliquefaciens bacteria liquid, ② Bacillus licheniformis bacteria liquid and ③ Brevibacillus lateralis bacteria liquid The weight ratio of ①, ② and ③ is (3~5):(1~2):(0.3~0.5). The preparation method is as follows: mixing the components. The application of the microbial fertilizer of the present invention in resisting the continuous cropping of peppers and cucumbers can inhibit the growth of root-knot nematodes, rapidly promote new roots, promote the absorption of water and nutrients by the roots, and promote the synthesis of chlorophyll when used for the resistance to repeated cropping of peppers. Improve yellowing; when used for resistance to continuous cucumber cropping, it can reduce the incidence of cucumber fusarium wilt, increase cucumber yield, and improve soil invertase, phosphatase, catalase, and urease activities, with significant effects.

This invention relates to Ailinofen bi lactone and its medical used salt or solvate. This invention also relates its preparation method and its medical combination and medical application. The compound in this invention can in vitro prevent six kinds of human body tumor cells activity as human original medullary cell leukemia cell(HL-60), nasopharyngeal carcinoma cell(CNE), oral epithelium cancer cell(KB), human lung cancer cell(549), human hepatic carcinoma cell(BEL-7404) and human uterus neck cancer cell(Hela). It can be developed as anti tumor medicine application.

The invention relates to a benzimidazole zinc complex and a preparation method thereof. A chemical name of the benzimidazole zinc complex is dichloro-2-(2-(1H-benzimidazole-2-)benzyl)-1H-benzimidazole zinc complex. The preparation method includes following steps: mixing 2-(carboxymethyl)benzoic acid with o-phenylenediamine, placing the mixture in phosphoric acid, performing a heating process at 180 DEG C for 3 hours to obtain a purple-green reactant, cooling the reactant to room temperature, adding distilled water, performing a neutralization reaction to obtain a yellow-green solid, performing a washing process through distilled water for three times, carrying out a filtering process, carrying out a recrystallization process through alcohol to obtain a yellow-green solid 2-(2-(1H-benzimidazole-2-)benzyl)-1H-benzimidazole, namely a ligand, dissolving ZnCl2 and the ligand 2-(2-(1H-benzimidazole-2-)benzyl)-1H-benzimidazole in a proper amount of a DMF solvent to obtain a mixture, stirring the mixture uniformly at room temperature, performing a volatilization process for seven days to obtain a slight yellow-green block-shaped complex monocrystal. The complex employs biological non-toxic element zinc as a metal ion, can be used for preparing antitumor drugs, can inhibit generation of esophagus cancer cells, and is free from remaining in human body and causing poisoning in human body due to metal accumulation. The complex is simple in the preparation method, is high in yield, is low in cost and is suitable for an industrial production.

The invention identifies junctional adhesion molecules A (JAM-A) highly expressed in peripheral blood lymphocytes of patients suffering from rheumatoid arthritis, proves that the highly-expressed JAM-A can effectively reduce migration of lymphocytes to peripheral bone joints, inhibit immunoreactions and joint inflammation, reduce bone destruction degree and relieve RA (rheumatoid arthritis) conditions, and relates to products in the fields of genetic engineering and biotechnological pharmaceutics. The production process of drugs made from the JAM-A is simple, mass production expansion is facilitated, technological conditions are easy to control, and production cycle is short. The invention mainly relates to application of JAM-A genes or protein encoded by the JAM-A genes, in particular toapplication of the JAM-A genes or the protein in genetic recombination drugs for treating the rheumatoid arthritis. The JAM-A genes are recombined with genetic engineering carriers to prepare the genetic recombination drugs capable of improving expression of the JAM-A, so that the rheumatoid arthritis can be treated effectively. The drugs can be in the form of injections or oral solutions.

Disclosed are conjugated diene derivatives, preparation method therefor, and uses thereof as anticancer drug. The structures of the conjugated diene derivatives are represented by general formula (I). Practices prove that the compounds have an activity of distinctly inhibiting the growth of tumor cells, and that the antitumor activity of some preferred compounds is distinctly superior to control drug. Furthermore, the preparation process for the compounds is simple and very practicable; raw materials can be easily obtained; the compounds are antitumor active substances having broad application prospects.