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Method and device for cultivating high-concentration photosynthetic bacteria with low illumination

A photosynthetic bacteria, high-concentration technology, applied in the field of microorganisms, can solve the problems of limited scale production and application, low concentration of photosynthetic bacteria, high-temperature sterilization of cathode and anode buffers, etc., to achieve enhanced growth of photosynthetic bacteria, easy operation, The effect of reducing energy consumption

Inactive Publication Date: 2014-03-05
重庆绿色智能技术研究院 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The patent with publication number CN101409355B discloses a photosynthetic microbial fuel cell, which can efficiently treat wastewater and produce electricity, but the concentration of photosynthetic bacteria obtained is low, only 280 million / mL, and the anode and cathode buffers require high temperature Sterilization, which limits its large-scale production and application

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  • Method and device for cultivating high-concentration photosynthetic bacteria with low illumination
  • Method and device for cultivating high-concentration photosynthetic bacteria with low illumination
  • Method and device for cultivating high-concentration photosynthetic bacteria with low illumination

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Embodiment 1

[0038] A device for cultivating high-concentration photosynthetic bacteria with low light intensity. The cultivation device includes a cathode chamber and an anode chamber. The anode chamber is made of transparent materials. The internal specifications of the two chambers are 5cm×5cm×5cm. The two chambers are separated by a cation exchange membrane. A silica gel pad is sandwiched between the cation exchange membrane and the bipolar chambers to keep the seal. Each chamber is filled with 120mL solution. The cathode chamber and the anode chamber are respectively equipped with a cathode and an anode, and are connected by an external resistor. Carbon felt is used as the electrode, and the size is 4cm×4cm, and the cathode uses carbon rod as the electrode. Add photosynthetic bacteria culture medium to the anode chamber, its composition is: sodium acetate 3g / L, NH 4 Cl1g / L, MgSO 4 0.2g / L, K 2 HPO 4 0.2g / L, NaHCO 3 1g / L, yeast powder 0.5g / L, pH7.4. Cathode chamber is added to cath...

Embodiment 2

[0043] The device for cultivating high-concentration photosynthetic bacteria with low light intensity is the same as in Example 1, except that the cathode chamber is an air cathode, the two pole chambers are separated by an anion exchange membrane, the external resistance resistance is set to 1000Ω, and 120mL of alcoholic waste water is added to the anode chamber, and its composition is : Chemical oxygen demand 10g / L, total volatile organic acids 6g / L, ammonia nitrogen 0.5g / L, pH7.2, cathode 0.5mg / cm 2 Platinum-loaded carbon paper is used as an electrode, and the size is 4cm×4cm. Inoculate photosynthetic bacteria with a volume percentage of 8% as a mixture of Rhodobacter sphaeroides and Rhodopseudomonas palustris with a ratio of 1:1, the concentration of the bacteria solution is about 3 billion / mL (logarithmic growth phase), and then It is cultivated under the conditions of 20-30°C and 300lux light intensity. During the cultivation process, the voltage is collected regularly b...

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Abstract

The invention discloses a method and a device for cultivating high-concentration photosynthetic bacteria with low illumination. The device comprises an anode chamber and a cathode chamber, wherein the anode chamber is made from a transparent material, and is separated from the cathode chamber through an ion exchange membrane; the anode chamber and the cathode chamber are respectively provided with an anode and a cathode; the anode and the cathode are connected through an external resistor; the ratio of surface area of the anode to effective capacity of the anode chamber is more than 0.06 / cm, and ratio of resistance of the external resistor to effective capacity of the anode chamber is 4-40 ohm / cm3; the anode chamber of the device is a fermentation tank; photosynthetic bacteria cultivated to a logarithmic phase are inoculated to the anode chamber at a volume percentage of 1-10%, and the anode chamber is sealed and is communicated to an external circuit to cultivate the bacteria at 20-37 DEG C with illumination of 50-500lux. The method disclosed by the invention can cultivate high-concentration photosynthetic bacteria under low inoculum size and low illumination, and can generate electric energy while reducing illumination energy consumption; and the method is wide in application prospect.

Description

technical field [0001] The invention belongs to the field of microorganisms, in particular to a device for cultivating high-concentration photosynthetic bacteria with low illumination and a method for cultivating high-concentration photosynthetic bacteria using the device. Background technique [0002] Photosynthetic bacteria (PSB for short) are prokaryotic organisms that first appeared on the earth, are ubiquitous in nature, and have a primitive photoenergy synthesis system. Harmful substances such as nitrate, the protein content of its cell dry matter is as high as 60%, and its protein amino acid composition is relatively complete. The cells also contain a variety of vitamins, especially the B vitamins are extremely rich, Vb2, folic acid, pantothenic acid, biological The content of carotene is also higher, and it also contains a large amount of physiologically active substances such as carotenoids and coenzyme Q. Therefore, photosynthetic bacteria can be widely used in fi...

Claims

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Application Information

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IPC IPC(8): C12M1/00C12N1/20H01M8/16C12R1/01
CPCY02E60/527C12M29/04C12M35/02C12M41/28C12N1/20H01M8/16Y02E60/50
Inventor 王兴祖任以伟巫忠胜田恩玲
Owner 重庆绿色智能技术研究院
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