Method and device for cultivating high-concentration photosynthetic bacteria with low illumination
A photosynthetic bacteria, high-concentration technology, applied in the field of microorganisms, can solve the problems of limited scale production and application, low concentration of photosynthetic bacteria, high-temperature sterilization of cathode and anode buffers, etc., to achieve enhanced growth of photosynthetic bacteria, easy operation, The effect of reducing energy consumption
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Embodiment 1
[0038] A device for cultivating high-concentration photosynthetic bacteria with low light intensity. The cultivation device includes a cathode chamber and an anode chamber. The anode chamber is made of transparent materials. The internal specifications of the two chambers are 5cm×5cm×5cm. The two chambers are separated by a cation exchange membrane. A silica gel pad is sandwiched between the cation exchange membrane and the bipolar chambers to keep the seal. Each chamber is filled with 120mL solution. The cathode chamber and the anode chamber are respectively equipped with a cathode and an anode, and are connected by an external resistor. Carbon felt is used as the electrode, and the size is 4cm×4cm, and the cathode uses carbon rod as the electrode. Add photosynthetic bacteria culture medium to the anode chamber, its composition is: sodium acetate 3g / L, NH 4 Cl1g / L, MgSO 4 0.2g / L, K 2 HPO 4 0.2g / L, NaHCO 3 1g / L, yeast powder 0.5g / L, pH7.4. Cathode chamber is added to cath...
Embodiment 2
[0043] The device for cultivating high-concentration photosynthetic bacteria with low light intensity is the same as in Example 1, except that the cathode chamber is an air cathode, the two pole chambers are separated by an anion exchange membrane, the external resistance resistance is set to 1000Ω, and 120mL of alcoholic waste water is added to the anode chamber, and its composition is : Chemical oxygen demand 10g / L, total volatile organic acids 6g / L, ammonia nitrogen 0.5g / L, pH7.2, cathode 0.5mg / cm 2 Platinum-loaded carbon paper is used as an electrode, and the size is 4cm×4cm. Inoculate photosynthetic bacteria with a volume percentage of 8% as a mixture of Rhodobacter sphaeroides and Rhodopseudomonas palustris with a ratio of 1:1, the concentration of the bacteria solution is about 3 billion / mL (logarithmic growth phase), and then It is cultivated under the conditions of 20-30°C and 300lux light intensity. During the cultivation process, the voltage is collected regularly b...
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