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Multi-epitope artificial antigen of plasmodium falciparum and application thereof

A technology of Plasmodium falciparum and its application, which is applied in the field of immune preparations against Plasmodium falciparum infection and the preparation of diagnostic reagents for Plasmodium falciparum infection, can solve problems such as antigenic variation, and achieve the effect of high inhibition efficiency and high immunogenicity

Active Publication Date: 2011-06-29
THE INST OF BASIC MEDICAL SCI OF CHINESE ACAD OF MEDICAL SCI
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  • Abstract
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  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Studies on the screened Plasmodium antigenic proteins have confirmed that their antigenicity will continue to mutate under the pressure of the host immune system, so currently no single use of any antigen can achieve the expected protective effect

Method used

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  • Multi-epitope artificial antigen of plasmodium falciparum and application thereof
  • Multi-epitope artificial antigen of plasmodium falciparum and application thereof
  • Multi-epitope artificial antigen of plasmodium falciparum and application thereof

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Experimental program
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Embodiment Construction

[0035] Materials, Methods and Results:

[0036] 1. Epitope gene synthesis

[0037] By consulting the literature, after analysis, it is determined to select the epitope sequences in Table 1, and at the same time, a specific spacer sequence is incorporated at both ends of each epitope gene sequence (introducing a start codon into the N-terminal epitope gene sequence, and introducing a start codon at the C-terminal Introducing a stop codon into the epitope gene sequence), according to a specific sequence (such as figure 2 ), the full-length gene sequence of M.RCAg-3 was synthesized by Shanghai Sangong Company with common codons, such as figure 1 SEQ ID No: 1 in.

[0038] Table 1. Epitope information table (sequences correspond to SEQ ID No: 5-14 respectively)

[0039] gauge

Antigen comes

source

epitope sequence

gauge

Types of

Amino

acid number

head

refer to

literature

E1

RESA

MQTLWDEIMD...

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PUM

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Abstract

The invention relates to a multi-epitope artificial antigen of plasmodium falciparum and an application thereof. The invention designs a polynucleotide with a sequence as shown in SEQ ID No:1 and a polypeptide with a sequence as shown in SEQ ID No:2, wherein the polynucleotide and the polypeptide are used as artificial antigens to prepare vaccines, and antibodies can be obtained from the antigens and are used to prepare immune preparations against plasmodium falciparum infections and kits for detecting malaria infections. Compared with anti plasmodium falciparum vaccines in the prior art, the multi-epitope artificial antigen of the invention has high immunogenicity and antigen recognition diversity, and the antibody of the invention has high inhibition efficiency on the in vitro growth of plasmodium falciparum.

Description

technical field [0001] The invention relates to the field of development and application of genes in biomedical high technology. Specifically, the present invention relates to an amino acid sequence of an artificial antigen with multiple epitopes that can be recognized by the serum of patients with falciparum malaria and a polynucleotide sequence encoding the amino acid sequence. The present invention also relates to a vaccine containing said amino acid sequence or said polynucleotide sequence. The present invention further relates to the use of the artificial antigen in the preparation of diagnostic reagents for Plasmodium falciparum infection and immune preparations against Plasmodium falciparum infection. Background technique [0002] Malaria is one of the deadliest epidemics in the world today. Nearly 4 billion people live in malaria-endemic areas worldwide, with approximately 247 million new infections each year and approximately 1 million deaths per year. Among the ...

Claims

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Application Information

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IPC IPC(8): C12N15/11C12N15/63C12N1/21C07K14/445C07K16/20A61K39/015A61K31/7088A61K39/395A61P33/06G01N33/569C12R1/19
CPCY02A50/30
Inventor 王恒曲久鑫
Owner THE INST OF BASIC MEDICAL SCI OF CHINESE ACAD OF MEDICAL SCI
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