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Stenotrophomonasmal-tophilia vaccine and its preparing method

A Pseudomonas preparation and Pseudomonas technology, which are applied in the field of microbial engineering, can solve the problems of high morbidity and mortality, and high mortality, so as to reduce the economic burden of patients, low prices, and low drug production costs. Effect

Inactive Publication Date: 2006-06-07
余国华
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] From the incidence rate of Pseudomonas infection, it can be found that it accounts for more than 30% of all clinical pathogenic bacterial infections in the past ten years. Although the antibiotics produced at home and abroad are changing rapidly, including cephalosporins, quinolones, and amino sugars, the present The morbidity and mortality of Pseudomonas maltophilia are still high, especially the mortality rate of Pseudomonas maltophilia is very high, accounting for about 35% of all Pseudomonas infection mortality
However, according to the recent information on the Internet, there has not been a specific and specific anti-Pseudomonas maltophilia special-effect preparation on the market at home and abroad. Based on this, it is considered that it is urgent to develop special-effect preparations against this bacterium at present, and this type of drug is very much needed in clinical practice.

Method used

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  • Stenotrophomonasmal-tophilia vaccine and its preparing method
  • Stenotrophomonasmal-tophilia vaccine and its preparing method
  • Stenotrophomonasmal-tophilia vaccine and its preparing method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0305] The name and source of the strain

[0306] Manufacture of Pseudomonas maltophilia strains Approved by the national drug regulatory authority, kept and distributed by the national drug control agency or a unit designated by the state.

[0307] Establishment of seed batches

[0308] The strains used for production should adopt the seed batch system. Prepare the working seed lot from the master seed lot. Working seed batches are used for production. Seed Batch Bacteria Verification

[0309] Morphology and Culture Assays

[0310] Inoculate the bacteria to be tested on broth agar, Martin agar or other suitable medium with pH 7.2-7.4, which should be Gram-negative bacillus. Pili, colonies are smooth, glossy edges are neat white-gray or light yellow.

[0311] biochemical reaction

[0312] Malonate citrate and DNA tests were positive, and oxidase was negative.

[0313] serum agglutination test

[0314] Cultivate the culture at 35°C for 18-20 hours, inactivate it with 1...

Embodiment 2

[0342] The name and source of the strain

[0343] The strains (Pseudomonas maltophilia) used for the manufacture of polysaccharide vaccines and the diagnostic serum used for testing should be approved by the national drug administration authority and distributed by the national drug testing agency or a country-designated unit.

[0344] The strains used for production should adopt the seed batch system. The main seed batch was lyophilized and preserved after amplification. Prepare the working seed lot from the master seed lot.

[0345] Working seed batches are used for production.

[0346] Verification of seed batches

[0347] For the identification of bacteria, broth agar base with pH7.2-7.4 or other suitable medium can be used.

[0348] Morphological and cultural characteristics

[0349] Inoculate the strains to be tested on the improved semi-comprehensive medium or other suitable medium, and culture at 35-37°C for 16-20 hours. The microscopic examination of the smear sh...

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Abstract

The present invention provides a biological product capable of resisting stenotrophomonas maltophilia and Pseudomonas aeruginosa infection and raising the body fluid immunity and cell immunity of malignant tumor patient. The biological product is prepared with stenotrophomonas maltophilia, and may be prepared into various kinds of immunological preparations and immune regulating preparations. The medicine of the present invention has the special effect of preventing and treating stenotrophomonas maltophilia and Pseudomonas aeruginosa infection and treating ascites carcinoma and leukemia.

Description

1. [Technical field] [0001] Pseudomonas maltrophilia (Pseudomonas maltrophilia), also known as Stenotrophomonas maltophilia preparation (vaccine), and a manufacturing method thereof belong to the field of microbial engineering. Utilize this bacterium body or metabolite (polysaccharide) etc. to produce specific therapeutic or preventive medicine. Two, [background technology] [0002] Pseudomonas maltophilia preparation (vaccine) and its manufacturing method, this application did not find any similar project or technology through Internet search, as far as we know, the prior art closest to this application can be found in "China Biological Products Regulations" 2000 Edition , "Pseudomonas aeruginosa manufacturing and testing regulations" P262-266, and "Pseudomonas preparations manufacturing and testing regulations" P272-276. However, the preparations of Pseudomonas aeruginosa or Pseudomonas are only used for treating tumors, and are ineffective for the prevention and treatmen...

Claims

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Application Information

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IPC IPC(8): A61K39/104A61K35/74A61P31/04
Inventor 余国华
Owner 余国华
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