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Livestock biological immune preparation and preparation method thereof

An immune preparation, a technology for livestock and poultry, applied in the field of animal husbandry, can solve the problems of invasion of various germs, increase the dosage of veterinary drugs, death, etc.

Pending Publication Date: 2022-02-18
黑龙江领康生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The composition of intestinal microbes has a crucial impact on immunity. The imbalance of intestinal flora can easily lead to low livestock production and death, and the decline in immunity will also lead to the invasion of various germs, affecting health and increasing veterinary drugs dosage, reduce the quality of poultry and livestock and surrounding products
In the prior art, oral probiotic preparations are mostly used to regulate the intestinal flora to promote the balance of the intestinal flora. It is a long process, relying on the self-regulation of poultry and livestock to slowly restore health, which seriously affects agricultural production

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] The biological immune preparation for poultry and livestock, the effective component of which is a specific yolk immunoglobulin complex antibody, and the preparation method of the specific yolk immunoglobulin complex antibody comprises the following steps:

[0021] (1) Escherichia coli k88, k99, 987P triple inactivated vaccines were used to immunize laying hens, and when the antibody titer reached 1:128, the highly immune eggs produced by laying hens were collected;

[0022] (2) Sterilize the high-free eggs collected in step (1) with 0.1wt% bromogeramine, and then separate the high-free egg yolks;

[0023] (3) Mix the egg yolk stock solution separated in step (2) with sterilized saline in equal volumes, then add chloroform of equal volume to the mixture of egg yolk stock solution and sterilized saline solution, shake fully and place in a water bath at 37°C for 30 minutes. Mix and oscillate once every 10 minutes, then centrifuge at 4000r / min for 20 minutes, collect the w...

Embodiment 2

[0025] The biological immune preparation for poultry and livestock, the effective component of which is a specific yolk immunoglobulin complex antibody, and the preparation method of the specific yolk immunoglobulin complex antibody comprises the following steps:

[0026] (1) Escherichia coli k88, k99, 987P triple inactivated vaccines were used to immunize laying hens, and when the antibody titer reached 1:150, the highly immune eggs produced by laying hens were collected;

[0027] (2) Sterilize the high-free eggs collected in step (1) with 0.1wt% bromogeramine, and then separate the high-free egg yolks;

[0028] (3) Mix the egg yolk stock solution separated in step (2) with sterilized saline in equal volumes, then add chloroform of equal volume to the mixture of egg yolk stock solution and sterilized saline solution, shake fully and place in a water bath at 37°C for 30 minutes. Mix and oscillate once every 10 minutes, then centrifuge at 4000r / min for 20 minutes, collect the w...

Embodiment 3

[0030] The biological immune preparation for poultry and livestock, the effective component of which is a specific yolk immunoglobulin complex antibody, and the preparation method of the specific yolk immunoglobulin complex antibody comprises the following steps:

[0031] (1) Escherichia coli k88, k99, 987P triple inactivated vaccines were used to immunize laying hens, and when the antibody titer reached 1:170, the highly immune eggs produced by laying hens were collected;

[0032] (2) Sterilize the high-free eggs collected in step (1) with 0.15wt% bromogeramine, and then separate the high-free egg yolks;

[0033] (3) Mix the egg yolk stock solution separated in step (2) with sterilized saline in equal volumes, then add chloroform of equal volume to the mixture of egg yolk stock solution and sterilized saline solution, shake fully and place in a water bath at 37°C for 30 minutes. Mix and oscillate once every 10 minutes, then centrifuge at 4000r / min for 20 minutes, collect the ...

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Abstract

The invention discloses a livestock biological immune preparation and a preparation method thereof. The effective component of the immune preparation is a specific yolk immunoglobulin composite antibody. According to the preparation method of the specific yolk immunoglobulin composite antibody, the specific yolk immunoglobulin composite antibody is prepared by taking three pathogenic bacteria k88, k99 and 987P of swine escherichia coli as antigens, and has a relatively good killing effect on three common pathogenic escherichia coli with high morbidity and high mortality in intestinal tracts. The traditional Chinese medicine composition can well regulate the balance of microbial flora in intestinal tracts, has a good prevention and treatment effect on livestock diarrhea, reduces the death rate of livestock, and is suitable for large-scale production and clinical application.

Description

technical field [0001] The invention relates to the technical field of animal husbandry, in particular to a poultry biological immune preparation and a preparation method thereof. Background technique [0002] The immune system of the animal body is an independent self-regulatory system, and various complex immune responses occur, develop, and subside within the immune system. The body's immune system has physiological functions such as immune defense, self-stabilization, and immune surveillance. The level of the body's immune function directly affects the body's disease resistance and productivity. [0003] The composition of intestinal microbes has a crucial impact on immunity. The imbalance of intestinal flora can easily lead to low livestock production and death, and the decline in immunity will also lead to the invasion of various germs, affecting health and increasing veterinary drugs The dosage will reduce the quality of poultry and livestock and surrounding product...

Claims

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Application Information

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IPC IPC(8): A61K39/40A61K39/395A61P1/00A61P31/04A61P1/12C07K16/02C07K16/12
CPCA61K39/40A61K39/395A61P1/00A61P31/04A61P1/12C07K16/1232C07K16/02C07K2317/11
Inventor 杨旭东张艳孙洪杰宋权超贾超
Owner 黑龙江领康生物科技有限公司
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