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A method for establishing an animal model of damp-heat asthma

An animal model and method-building technology, applied in pharmaceutical formulations, compound screening/testing, and resistance to vector-borne diseases, etc., can solve the problems that cannot reflect the practical significance of the application of traditional Chinese medicine syndrome differentiation, cannot incorporate damp-heat factors, and cannot implement the thought of traditional Chinese medicine syndrome differentiation and treatment. Implementation and other issues, to achieve the effect of convenient research, high modeling success rate, and good model repeatability

Inactive Publication Date: 2017-06-16
季旭明
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the traditional asthma model only considers the factors of external antigens, and cannot incorporate the factors of dampness and heat into the model. Therefore, it is impossible to accurately implement the TCM syndrome differentiation and treatment ideas in the animal experimental research process, and it cannot reflect the practical significance of the application of TCM syndrome differentiation and formulating prescriptions.

Method used

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  • A method for establishing an animal model of damp-heat asthma
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Examples

Experimental program
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Effect test

Embodiment 1

[0028] 1. Required materials

[0029] 1.1 Experimental animals

[0030] 20 SPF grade rats (Experimental Animal Center of Shandong University of Traditional Chinese Medicine, license number: SCXK (Ji) 2008-1-003, certificate number: 1011003) weighing 150 ± 10g, about 1-1.5 months old.

[0031] 1.2 Experimental drugs

[0032] Ovalbumin dry powder: OVA (Ovalbumin), 1g / bottle, 5g / bottle, subpackaged by Beijing Dingsheng Changsheng Biotechnology Co., Ltd., and prepared into 10% and 2% solutions before use.

[0033] Dried strains of invasive Escherichia coli: purchased from the strain room of Shandong Health and Epidemic Prevention Station, strain number 8099.

[0034] Aluminum hydroxide powder: analytically pure AR, 500g / bottle, Tianjin Hengxing Chemical Reagent Manufacturing Co., Ltd., production date: March 1, 2013.

[0035] Chloral hydrate: analytically pure AR, 250g / bottle, from Tianjin Kemiou Chemical Reagent Co., Ltd., batch number 201001100, prepared into a 10% solution w...

Embodiment 2

[0101] Using the method of the above-mentioned Example 1, 10 batches of animal models were established continuously, with 20 model rats in each batch. During the modeling process, a total of 8 rats died, and the time of death was concentrated during the period when they were placed in the artificial climate chamber. After the modeling was completed, the surviving mice were tested for various performance indicators of the animal model according to the method in Example 1. The results showed that 100% of the surviving rats showed good symptoms of damp-heat asthma, indicating that the model was successfully established.

[0102] It can be seen that the method of the present invention has high success rate, good reproducibility, low rat mortality rate, and has promotional value.

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Abstract

The invention discloses a building method of a dampness-heat asthma animal model. The method comprises the following steps of using high-fat diet to feed a rat, filling colibacillosis, and forming a dampness-heat syndrome type of the rat; when the high-fat diet is fed, using albumen and aluminium hydroxide normal saline mixing suspension liquid to sensitize the rat; after sensitizing, activating by the albumen normal saline solution, so as to obtain the dampness-heat asthma animal model. The method has the advantage that the traditional asthma animal model is organically combined with the traditional Chinese dampness-heat syndrome type animal model to form the traditional Chinese dampness-heat syndrome type asthma animal model, so as to apply for the traditional Chinese clinical asthma study, and facilitate the study of traditional Chinese asthma relieving medicine sieving.

Description

technical field [0001] The invention relates to the technical field of animal pharmacological experiments, in particular to a method for establishing and detecting an animal model of damp-heat asthma. Background technique [0002] The etiology and pathogenesis of allergic asthma is the type I allergic reaction caused by external antigens entering the body. The animal model prepared according to the basic principle that foreign antigens enter the body and cause type I allergy has become an important tool for researching the safety evaluation of antiasthmatic drugs and biological products and the screening of effective drugs. The occurrence of asthma is mostly due to chronic diseases lying in the lungs, which are triggered by exogenous factors, diet, emotion, fatigue and other inducements, resulting in phlegm blocking the airway and lung loss. However, due to changes in climate and dietary structure, people who eat fat, sweet, thick, greasy, spicy and fried food tend to accum...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01K67/027A61K35/74A61K38/17
CPCA01K67/02A01K2207/25A01K2267/03A61K49/0008Y02A50/30
Inventor 季旭明张桂菊吴金勇袭雷鸣卢立伟李燕宁
Owner 季旭明
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