78 results about "Immune defense" patented technology

The present invention relates to compositions and methods for regulating an immune response in a subject, particularly to treat a subject with a tumor, notably a solid tumor, or an infectious disease. Disclosed are methods of regulating the innate immunity in a subject, such as by regulating the activity of γδ T cells in a subject. Disclosed are combinations of particular agents, such as a cytokine and a γδ T cell activator, particular regimens and dosages can produce a remarkable expansion of γδ T cells in vivo and a remarkable increase in a subject's immune defense. The invention can be used for therapeutic purposes, to produce, regulate or facilitate an immune response in a subject.

The invention relates to milk powder conducive to strengthening immunity and improving resistance. The milk powder is prepared from vitamin A, vitamin C, vitamin E, B-group vitamin, calcium, iron, zinc, selenium, N-acetylneuraminic acid, lactoferrin, bovine colostrum powder, and yeast beta-glucan. According to the milk powder, by providing essential nutrients for the three immune defense lines of''skin and mucosa'', ''non-specific immunity'' and ''specific immunity'', the strength of an immune system is enhanced, and the resistance is improved.

The invention provides an antagonistic peptide which can simulate the key sequence for bonding MD2 and LPS and competitively antagonize the specific bonding which is generated by LPS and MD2, thus achieving the mitigation of the excessive activation of the immune inflammatory cells of LPS and further easing the caused excessive septic damages to the body. The amino acid sequence of the antagonist peptide includes: a sequence 1 of Lys Thr Val Pro Asp Asn His; and the sequence 2 of Ile Gly Lys Phe Leu Tyr Arg. The peptide can effectively mitigate the septic inflammation damage which is induced by the intracellular toxin LPS, thus significantly improving the survival rate of mouses which are infected by the intracellular toxin and maintaining the body immune defense function at the same time of specifically inhibiting the excessive stimulation of the LPS on the inflammatory cells. The antagonistic peptide can be used for preparing the treatment drugs of the inflammatory diseases which are caused by gram-negative bacteria and are related to a toll-like receptor protein.

The invention relates to the technical field of molecular biology, in particular to eriocheir sinensis Crustin-1 gene clone and a recombination expression technology thereof. Crustin-1 gene of which the cDNA span is 821bp is cloned from eriocheir sinensis by using an expressed sequence tag (EST) technology and a 3' and 5' end rapid augmentation technology (RACE) and comprises a 315bp open reading frame, polyadenylic acid tailing signals and polyadenylic acid tails, 104 amino acids are coded, the length of a 5' non-coding region is 239bp, the length of a 3' non-coding region is 267bp, and the Crustin-1gene play an important role in the immune defense aspect of the eriocheir sinensis. The invention obtains eriocheir sinensis Crustin-1 protein by using the in-vitro recombination expression technology, the recombination protein has stronger bacteriostatic activity for gram-positive bacteria, the minimal inhibitory concentrations of the recombination protein for micrococcus luteus, bacillus subtilis, micrococcus tetragenus and bacillus thuringiensis are 0.12 mu m, 0.23 mu m, 0.46 mu m and 0.12 mu m respectively, and the recombination protein does not have obvious inhibiting function. The invention can lay the foundation for the disease control of the eriocheir sinensis, the gene assistant breeding and the development of feed additives.

The invention relates to lysozyme, specifically to a Mytilus edulis G-type lysozyme gene and a recombinant protein and application thereof. The G-type lysozyme gene has a base sequence as represented by SEQ ID No. 1 in a sequence table. Through PCR technology, a gene fragment coding the mature peptide of the G-type lysozyme gene is amplified and cloned into a pET21(a) expression vector, and prokaryotic in-vitro recombinant expression in escherichia coli BL21(DE3)plysS is realized. After Ni-affinity chromatographic purification and dialysis renaturation, a recombination product has inhibitory effects on a variety of Gram positive and negative pathogens and especially has a substantial effect on pseudomonas putida, and minimal inhibitory concentration is 2.39 to 4.79 mu mol / L. The invention provides bases for further research on immune defense mechanism of economic shellfish and lays a foundation for disease control, genetic breeding and feed additives for aquatic livestock.

The invention provides a vagina care composition and application thereof, a female private care solution containing the vagina care composition and a preparation method of the female private care solution and relates to the technical field of female private care. The vagina care composition is mainly prepared from tremella polysaccharide and lactobacillus fermented lysate; the weight ratio of thetremella polysaccharide to the lactobacillus fermented lysate is (1 to 2) to 100. The tremella polysaccharide can be used for effectively improving the body immunity function and inhibiting cell lipidperoxidation; the lactobacillus fermented lysate contains broad-spectrum nisin and has an obvious containment effect on gram positive bacteria; the lactobacillus fermented lysate can be used for promoting the proliferation of like probiotics. The composition is prepared by compounding the tremella polysaccharide and the lactobacillus fermented lysate; the composition has cleaning and bacterium-inhibiting effects and also has the effects of regulating the imbalance of colony of female private parts, inhibiting the peroxidation of cell lipids and improving the vagina self-cleaning function andthe immune defense function.

A Chinese herbal medicine compound polysaccharide immunity-enhanced feed additive for prawns has the characteristic of enhancing immunity and disease resistance of the prawns. The additive is preparedfrom active ingredients of Chinese herbal medicines in percentage by mass as follows: 40%-70% of astragalus polysaccharide, 10%-20% of rhizoma atractylodis macrocephalae polysaccharide and 10%-40% ofsaposhnikovia divaricata polysaccharide. The additive is simple to prepare, lower in cost and convenient to use. After being produced and applied, the preparation can effectively improve the immune defense capacity of the prawns, prevent and treat diseases of the prawns and reduce use of antibiotics and chemical drugs, meanwhile, the problems of effective component uncertainty, multiple impurities, poor palatability and the like caused by addition of original powder of Chinese herbal medicines are solved, and technical support is provided for green and healthy culture of the prawns.

The invention discloses a composite immunoenhancement feed additive for litopenaeus vannamei and an application and feed thereof. The composite immunoenhancement feed additive for litopenaeus vannamei comprises beta-dextran and selenium methionine; when selenium methionine is calculated on the basis of the selenium amount, the mass ratio of beta-dextran to selenium methionine is 150-300:0.2. According to the invention, experiments show that common basal feed for litopenaeus vannamei is added with the combination of beta-dextran and selenium methionine according to a certain mass ratio, or the combination of beta-dextran and vitamin E according to a certain mass ratio, or the combination of beta-dextran, selenium methionine and vitamin E according to a certain mass ratio; through the antioxidation approach of selenium methionine and vitamin E, the immune defense capability of litopenaeus vannamei bodies is improved; the immunostimulation time of beta-dextran is effectively prolonged; the using amount is decreased; and the immune fatigue problem generated when beta-dextran is used alone is solved. The additive of the invention can be added into basic ration of litopenaeus vannamei directly, and is convenient for use.

The invention discloses a power terminal and an information safety protection method and system of an embedded system of the power terminal, information safety protection is actively carried out through immune self-stabilization, immune monitoring and immune defense modes according to an artificial immune principle, and the method comprises the following steps: S1, establishing an embedded systemendogenous credible operation environment for immune self-stabilization; S2, loading an embedded system abnormality detection logic inspection program for immunomonitoring; S3, selecting whether to load a fault emergency processing and event recording program for immune defense or not according to the result checked in the step S2: if the result checked in the step S2 is abnormal, loading the fault emergency processing and event recording program for immune defense; otherwise, not loading. According to the invention, three major functions of immune self-stabilization, immune monitoring and immune defense of human immunity are simulated, the power terminal is endowed with an anthropomorphic active immune safety protection capability, the information safety of the power industrial control terminal is maintained to a certain extent, and the safety risk of the power terminal is reduced.

The present invention relates to blood coagulation factor XIII (FXIII) for treatment and / or prevention of an infection by a microorganism and / or the symptoms associated with said infection, a pharmaceutical composition comprising a pharmaceutically effective amount of said FXIII, a method for the manufacture of a medicament comprising a pharmaceutically effective amount of said FXIII, and a method of treatment comprising administering to a patient in need a pharmaceutically effective amount of said FXIII.

The invention discloses a prezatide copper acetate essence and a preparation method thereof. The prezatide copper acetate essence comprise the following raw materials: water, glycerol, hydrolyzed collagen, microalgae parvum polysaccharide, maltooligosaccharide glucoside, hydrogenated starch hydrolysate, tripeptide-1 copper, acetyl hexapeptide-8, dipeptide-1, dipeptide-2, sodium hyaluronate, trehalose, a cactus tangutorum stem extract, a zanthoxylum bungeanum fruit extract, 1,2-pentanediol, a pomelo extract, a witchhazel extract, arginine and imidazolidinyl urea. The prezatide copper acetate essence is safe and healthy, and has effects of wrinkle resistance, aging resistance, firmness, moistening, oxidation resistance, whitening, repair regeneration, red luster and immune defense when usedon skin. The loose skin can be tightened, so that the elasticity of the skin is improved; and photodamage is relieved, and spots are removed.

The invention provides alcohol effect-dispelling and liver-protecting food for improving immunity and a preparation method thereof. The food is prepared from the following components in parts by mass:1-50 parts of herba cepbalanoplosis segeti extract, 1-50 parts of extract of root of kudzu vine, 1-10 parts of dandelion extract, 1-10 parts of conifer cherry extract, 10-500 parts of isomalt and 1-10 parts of curcuma powder. The alcohol effect-dispelling and liver-protecting food for improving immunity can alleviate and resist damage of alcohol to the liver from multiple aspects of alcohol effect dispelling, liver protecting, auxiliary adjusting and the like, improves the detoxifying function and the metabolic function of the liver and the functions of secreting bile and immune defense. Thecandy is good in taste and convenient to take, so that a novel eating mode is provide for the public.

The invention relates to an antiviral polypeptide and a preparation method and application of the antiviral polypeptide. The antiviral polypeptide contains any one amino acid sequence shown in SEQ IDNO.1-SEQ ID NO.8, the antiviral polypeptide gene is transformed into a plant and has a remarkable antiviral effect, and the antiviral polypeptide gene has a relatively strong antiviral effect on various viruses such as cucumber mosaic virus and barley stripe mosaic virus. The antiviral polypeptide is prepared by adopting a yeast expression system, the expression polypeptide is sprayed to a surfaceof the plant, and the virus infection amount of the plant is remarkably reduced. The antiviral polypeptide provided by the invention can be used for preventing and treating various virus infection ofplants; plant protection is carried out in production by utilizing a biological agent, and effective plant immune defense is realized, so that the use amount of pesticides is reduced, the plant quality is effectively improved, the yield of the plant is effectively increased, and the economic and social effects of crops are improved.

This invention provides tools and methods that prevent a cancer cell from growing and reproducing more cancer cells. When growth ceases the body's immune defenses are enabled to attack and destroy these cells if the cancer cell itself has not initiated its own natural apoptotic self-destruction processes. The tools and methods of the invention obstruct the metabolic adaptions required to support cancer growth. By addressing the increased rates of metabolism characteristic of all rapidly reproducing cancer cells using chemical and / or physical nanotechnology to identify, segregate, isolate these hypermetabolizing cells, the body's immune system and other natural defenses are empowered to further isolate and eliminate the diseased cells. The extreme growth rates required for their rapid reproduction involve massively increased rates of the biochemical reactions supporting the cancerous growth. Each excess reaction produces extra heat and raises the internal cell's temperature and the tissue space immediate to the rapidly growing cells. This heat signature is used as a primary biomarker that enables binding of a nanoviral particle engineered to migrate to at attach at the target site at the site and prevent the cell from continued metabolism. Preferably, the nanoparticle not only binds and blocks external membrane receptors on the target cell, but incorporates into the rapidly metabolizing cells additional metabolic blocking agents to stop their growth. When cell growth and proliferation are stopped, the body's natural defenses are able to segregate and eliminate these cells. The massively increased rates of metabolic reactions characteristic of cancer cells also produce excess acid. The decreased pH is useful as a secondary or confirmatory marker for identifying these cancer cells.

The invention relates to a traditional Chinese medicine composition for treating nasopharyngeal carcinoma. The selected medicinal materials comprise gardenia, fructus forsythia, bombyx batryticatus, flos magnolia, radix scrophulariae, white paeony root, fructus xanthii, radix glehniae, seaweed, fresh kelp, selfheal, radix sophorae tonkinensis and elephantopus scaber. The traditional Chinese medicine composition is used for treating nasopharyngeal carcinoma and constructing a strong immune defense barrier, and is significant in effect, free of a toxic or side effect, low in cost and capable of inhibiting relapse.

The invention belongs to the technical field of molecular biology, in particular relates to a Portunus trituberculatus anti-lipopolysaccharide factor PtALF-3 gene and encoding proteins and application thereof. In the invention, a PtALF-3 gene cDNA is amplified from a Portunus trituberculatus by utilizing a cDNA (Complementary Deoxyribonucleic Acid) library and an RACE (Rapid-amplification of cDNA Ends) technology and plays an important role in immune defense of the Portunus trituberculatus. A recombinant PtALF-3 protein has obvious antibacterial activity to Gram-negative Vibrio alginolyticus, P. Aeruginosa, Edwardsiella tarda, Gram-positive Staphylococcus aureus, Micrococcus luteus and fungus Pichia pastoris, and the minimal inhibitory concentrations are respectively 0.58 mu M, 0.58 mu M, 9.26 mu M, 74.08 mu M, 18.52 mu M and 9.26 mu M. The invention lays a foundation for disease prevention and control, gene-assisted selection and development of feed additives of the Portunus trituberculatus.

The present invention discloses a Chinese herbal medicinal preparation with stable medicine properties for immunological enhancement and sterilization of penaeus orientalis and a method thereof. The Chinese herbal medicinal preparation comprises the following ingredients in mass percentages: 20%-50% of astragalus polysaccharide, 20%-50% of chlorogenic acid, 20%-50% of allicin, 5%-10% of sodium hydrogen sulfite, and 3%-6% of dimethyl-beta-propionic acid thialdine. The method comprises the following steps: radix astragali is crushed, the crushed radix astragali is screened, the screened radix astragali is mixed evenly with distilled water to be boiled, filtrate is concentrated, ethanol is added until the ethanol content is 80%, and the materials are subjected to suction filtration overnight to obtain the astragalus polysaccharide; cortex eucommiae is crushed, the crushed cortex eucommiae is screened, the screened cortex eucommiae is mixed evenly with ethyl acetate, the mixture is subjected to stirring and reflux extracting at water bath at 65 DEG C, filtrates are blended, ethyl acetate is separated, and the chlorogenic acid is obtained. The Chinese herbal medicinal preparation is simple in preparation processes, relatively low in costs and convenient in applications, can effectively improve the immune defense ability and sterilization function of the penaeus orientalis after the production application, effectively prevents and cures diseases of the penaeus orientalis, reduces the uses of antibiotics and chemical drugs, provides technical supports for sustainable development of breeding health of the penaeus orientalis, and provides technical supports for ecological environment protection of water areas.

The invention discloses hemoglobin gene of macrobrachium nipponensis, a clone method of the hemoglobin gene, and a preparation method of recombinant protein. The hemoglobin gene of macrobrachium nipponensis is cloned through an expressed sequence tag technology and 3'and 5' rapid amplification cDNA end (RACE), wherein the overall length of the cDNA is 1201bp, the gene has a 579bp open reading frame and encodes 193 amino acids, 5' noncoding region is 199bp and 3' noncoding region is 420bp, and the gene has polyadenylic acid tailing signal and polyadenylic acid tail. The gene plays an important role in immune defense of macrobrachium nipponensis. The hemoglobin gene of macrobrachium nipponensis is obtained by in vitro recombination expression; the recombinant protein not only can carry oxygen, but also has bacteriostasis, thereby providing theoretical basis for disease control and gene-assisted breeding of macrobrachium nipponensis,and development of feed additives for macrobrachium nipponensis.

The invention belongs to the technical field of molecular biology, in particular relates to a Portunus trituberculatus anti-lipopolysaccharide factor PtALF-5 gene and encoding proteins and application thereof. In the invention, a PtALF-5 gene cDNA is amplified from a Portunus trituberculatus by utilizing a cDNA (Complementary Deoxyribonucleic Acid) library and an RACE (Rapid-amplification of cDNA Ends) technology and plays an important role in immune defense of the Portunus trituberculatus. A recombinant PtALF-5 protein has stronger antibacterial activity to Gram-negative Vibrio alginolyticus and P. Aeruginosa, and the minimal inhibitory concentrations are respectively 7.77 mu M and 31.08 mu M; but the recombinant PtALF-2 protein has no obvious inhibitory action to Gram-positive Staphylococcus aureus, Micrococcus luteus and fungus Pichia pastoris. The invention lays a foundation for disease prevention and control, gene-assisted selection and development of feed additives of the Portunus trituberculatus.

The invention belongs to the technical field of molecular biology, in particular relates to a Portunus trituberculatus anti-lipopolysaccharide factor PtALF-2 gene and encoding proteins and application thereof. In the invention, a PtALF-2 gene cDNA is amplified from a Portunus trituberculatus by utilizing a cDNA (Complementary Deoxyribonucleic Acid) library and an RACE (Rapid-amplification of cDNA Ends) technology and plays an important role in immune defense of the Portunus trituberculatus. A recombinant PtALF-2 protein has obvious antibacterial activity to Gram-negative Vibrio alginolyticus, P. Aeruginosa, Edwardsiella tarda, Gram-positive Staphylococcus aureus and Micrococcus luteus, and the minimal inhibitory concentrations are respectively 1.02 mu M, 2.04 mu M, 4.07 mu M, 16.30 mu M and 65.19 mu M; but the recombinant PtALF-2 protein has no obvious inhibitory action to fungus Pichia pastoris. The invention lays a foundation for disease prevention and control, gene-assisted selection and development of feed additives of the Portunus trituberculatus.

Phenolic compounds with a phenolic molecule to which are covalently linked an oxygen-containing group, a nitrogen or another oxygen containing group, and a C1-C4 alkoxy group, obtainable from monocotyledonous plants, or by chemical synthesis, have been found to preserve and / or augment innate immune defenses in humans and animals. Additional chemical compounds of the present invention may include benzoxazinoids-cyclic hydroxyamic acids, lactams, and corresponding glucosides, which may serve as precursors to phenolic compounds. The phenolic compounds and precursors of phenolic compounds of the present invention, at concentrations suitable for human and animal therapeutic use, may be obtained from monocotyledonous plants such as corn in their early growth states which are timely harvested for optimum yield.

The invention relates to the technical field of molecular biology, in particular gene clone of Eriocheir sinensis Crustin-2 and recombinant expression technology. The invention uses an expressed sequence tag (EST) technique and 5' and 3' tail end rapid amplification technique (RACE) to clone a Crustin-2 gene from Eriocheir sinensis, wherein the cDNA has a full length of 1,255 bp comprising a 339p pen reading frame to code 112 amino acids, and the gene plays an important role in immune defense of the Eriocheir sinensis. A recombinant Crustin-2 protein has remarkable gram-positive bacterium antibacterial activity, has the minimum bacteriostatic concentrations of 0.093 mu M, 0.19 mu M, 0.37 mu M and 0.093 mu M to Micrococcus luteus, Bacillus subtilis, Mlicrococcus tetragenus and Bacillus thuringiensis respectively, and has no obvious antibacterial activity to a gram-negative bacterium. The invention can provide a foundation for the further research on the immune defense mechanism of the Eriocheir sinensis, and lays a foundation for disease control, gene-assisted breeding and feed additive development of the Eriocheir sinensis.

The invention discloses a thymol-containing termite control medicine and application thereof. The termite control medicine contains thymol and entomopathogenic nematodes. In order to enhance biological prevention and control of termites and improve application of entomopathogenic nematodes in prevention and control of termites, plant-derived active substances which are low in toxicity, renewable and not prone to generating resistance are used as interference inhibitors and are combined with the entomopathogenic nematodes to prevent and control harmful termites. According to the application disclosed by the invention, the most important harmful termite Coptotermes formosanus in China is taken as a test object, the control effect of the entomopathogenic nematodes on the Coptotermes formosanus is improved based on the interference of a plant-derived active substance, namely thymol (5-methyl-2-isopropynol) on immune defense response of the termites as well as infection of an entomopathogenic nematode Steinomarpocapsa All strain, and the application has important significance for further enhancing application and popularization of entomopathogenic nematodes in green prevention and control of termites.

The present invention relates to novel therapeutic uses of iron(III) complex compounds with carbohydrates or derivatives thereof, in the preparation of a medicament for improving immune defense and / or brain performance.

The invention provides an antifungal gene, polypeptide and recombinant protein as well as a preparation method and an application thereof, the antifungal gene is a Ruditapes philippinarum antifungal Rpmacin gene, and a nucleotide sequence of the Rpmacin gene is shown as SEQ ID NO.1; the amino acid sequence of the polypeptide is as shown in SEQ ID NO. 2; the recombinant protein contains the antifungal gene and expresses the antifungal polypeptide. The antifungal gene, polypeptide and recombinant protein provided by the invention can be applied to production of antibacterial drugs, vaccines or feed additives for vegetables, livestock, poultry and aquatic products, and have broad application prospects. The gene, polypeptide and recombinant protein as well as the preparation method and the application thereof can provide a basis for further researching the immune defense mechanism of Ruditapes philippinarum, and lay a foundation for disease control, genetic breeding and feed additive research of Ruditapes philippinarum.

The invention discloses an artificial breeding method of an anti-edwardsielliasis excellent variety of paralichthys olivaceus. The method specifically comprises the steps that a pond with the water depth of 2-3 m is selected, and a net cage for breeeding paralichthys olivaceus is arranged; a strain is inoculated into a liquid culture medium for breeding, and centrifuging is conducted; a bacterial solution with the medial lethal concentration of 0.18-0.22 mL / 10g is subjected to intraperitoneal injection for breeding; hybridization, selfing and gynogenesis are used for developing offsprings, the offsprings are bred, and the anti-edwardsielliasis paralichthys olivaceus is obtained. The artificial breeding method has the advantages that the anti-disease variety is bred with the cooperation of family selection and the modern biological technology, which is an important means for solving the paralichthys olivaceus disease problem; a Chinese herbal medicine insecticide is added in the breeding process, an antibacterial effect is achieved on the edwardsiella tarda, residues are few, the drug resistance of pathogenic bacteria is not likely to be caused, the anti-stress capability of organisms can be mobilized, and the immune defense function of the organisms can be improved.

The invention discloses a compound additive for providing a comprehensive health-care function for pet food. The compound additive is characterized by comprising, by weight, 81% of fish oil, 16.5% ofyeast cell walls and 2.5% of aloe. Effective substances are extracted from the yeast cell walls, the aloe and the fish oil, the problem of heat stability is effectively solved, and the additive has the functions of helping pets inhibit free radicals, reducing oxidative damage, activating and improving immune defense, absorbing pathogenic bacteria and mycotoxins, strengthening the digestive system,nourishing clothing hair, promoting healing, and comprehensively helping the pets keep healthy.

The invention provides an application of a drug composition in the preparation of drugs for preventing salmonella infection in laying hens, improving egg laying performance of the laying hens, reducing the amount of salmonella in shells and contents of eggs and improving quality of the eggs of the laying hens. The drug composition comprises, by weight, 20-50 parts of gallnut tannic acid and 10-30parts of glycine. The drug composition has the advantages of green safety and no tolerance by taking 'source conditioning' as the core; replacing antibiotics, the drug composition can effectively inhibit and kill salmonella, eliminate endotoxemia caused by bacterial death and release of endotoxin, adjust the body's immune defense, purify salmonella from the laying hens, reduce horizontal spread ofsalmonella and egg-borne contamination caused by potential endogenous displacement of chickens, improve egg laying performance and the quality of the eggs and effectively avoid food safety problems caused by the egg-derived salmonella.

The invention relates to a compound feed additive and application thereof and provides a compound immunity growth enhancer for prawns and application thereof. In addition, the invention also providesa feed containing the compound immunity growth enhancer for prawns. The compound immunity growth enhancer for prawns is prepared from, by mass, 10%-30% of vitamin C, 5%-20% of vitamin E and 50%-80% ofbile acid. The compound immunity growth enhancer for prawns has the effects of promoting prawn growth and improving immune defense ability and bactericidal effect of the prawns, effectively improvesfat metabolism of the prawns, promotes prawn growth, decreases the use amount of antibiotics and chemicals, can obviously improve the immune defense ability and growth rate of prawns, can promote protein and fat metabolism of prawns and improve the immune defense ability of prawns and provides technical support for healthy sustainable development of prawn breeding.