The invention discloses a prezatide copper acetate essence and a preparation method thereof. The prezatide copper acetate essence comprise the following raw materials: water, glycerol, hydrolyzed collagen, microalgae parvum polysaccharide, maltooligosaccharide glucoside, hydrogenated starch hydrolysate, tripeptide-1 copper, acetyl hexapeptide-8, dipeptide-1, dipeptide-2, sodium hyaluronate, trehalose, a cactus tangutorum stem extract, a zanthoxylum bungeanum fruit extract, 1,2-pentanediol, a pomelo extract, a witchhazel extract, arginine and imidazolidinyl urea. The prezatide copper acetate essence is safe and healthy, and has effects of wrinkle resistance, aging resistance, firmness, moistening, oxidation resistance, whitening, repair regeneration, red luster and immune defense when usedon skin. The loose skin can be tightened, so that the elasticity of the skin is improved; and photodamage is relieved, and spots are removed.