Thymol-containing termite control medicine and application thereof

A technology of thymol and termites, which is applied in application, animal repellent, plant growth regulator, etc., can solve the problems of the complex living environment of termites, the lack of understanding of the immune mechanism of termite defense against nematodes, and the poor effect of nematodes.

Active Publication Date: 2021-08-27
INST OF ZOOLOGY GUANGDONG ACAD OF SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the 1970s, researchers began to use entomopathogenic nematodes to control termites, which have the potential to be used as termite control agents. However, due to factors such as termite social behavior, physiological and biochemical defense responses, nematodes are not effective in actual termite control applications (Chouvenc et al. al., 2011; Al-Zaidawi et al., 2020)
At present, the research on the immune defense mechanism of termites mainly focuses on the research on termites' defense against bacteria and fungi. The living environment of termites is complex and the immune defense mechanism has not yet been elucidated. Little is known about the immune mechanism of termites against nematodes.

Method used

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  • Thymol-containing termite control medicine and application thereof
  • Thymol-containing termite control medicine and application thereof
  • Thymol-containing termite control medicine and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0032] 1. Test method

[0033] 1. Thymol's ability to survive and infect nematodes

[0034] In the suspension mixed with thymol and nematode liquid, the concentration of nematodes is 200IJ / only (26667IJ / mL), and the concentrations of thymol are respectively 0.141mg / mL, 0.166mg / mL, and 0.186mg / mL (denoted as LD10, LD20, LD30), each treatment was repeated three times, and the nematode suspension (CK) without adding thymol was used as the control. Place in a dark incubator at 25±1°C for 7 days, observe every day, randomly take 100 μL of nematode suspension for each repetition, put it in a counting plate, and check the survival of nematodes under a stereoscope. Calculation: Survival rate%=(number of living nematodes÷number of total nematodes)*100%.

[0035] Pick 10 Mellonella mellonella larvae in a 9cm petri dish with two layers of filter paper, drop 1.5mL of the above mixed nematode suspension into each dish, seal it with parafilm, and place it in a dark incubator at 25±1°C for...

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Abstract

The invention discloses a thymol-containing termite control medicine and application thereof. The termite control medicine contains thymol and entomopathogenic nematodes. In order to enhance biological prevention and control of termites and improve application of entomopathogenic nematodes in prevention and control of termites, plant-derived active substances which are low in toxicity, renewable and not prone to generating resistance are used as interference inhibitors and are combined with the entomopathogenic nematodes to prevent and control harmful termites. According to the application disclosed by the invention, the most important harmful termite Coptotermes formosanus in China is taken as a test object, the control effect of the entomopathogenic nematodes on the Coptotermes formosanus is improved based on the interference of a plant-derived active substance, namely thymol (5-methyl-2-isopropynol) on immune defense response of the termites as well as infection of an entomopathogenic nematode Steinomarpocapsa All strain, and the application has important significance for further enhancing application and popularization of entomopathogenic nematodes in green prevention and control of termites.

Description

Technical field: [0001] The invention belongs to the field of termite control, and in particular relates to a termite control drug containing thymol and application thereof. Background technique: [0002] Termites are a worldwide pest, listed as one of the top five worldwide pests by the International Insect Physiology and Ecology Research Center, which can cause serious harm to various fields such as houses, buildings, reservoirs, dams, transportation, and agricultural production. The occurrence is particularly severe in subtropical regions (Huang Fusheng et al., 2000). Entomopathogenic nematodes (EPNs) are one of the important biological control agents due to their ability to actively search for hosts, broad-spectrum insecticidal properties, and less resistance to pesticides. In the 1970s, researchers began to use entomopathogenic nematodes to control termites, which have the potential to be used as termite control agents. However, due to factors such as termite social be...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N63/12A01N31/08A01P7/04
CPCA01N63/12A01N31/08Y02A50/30
Inventor 曾文慧李志强吴文静刘炳荣陈彤
Owner INST OF ZOOLOGY GUANGDONG ACAD OF SCI
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