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Artificial breeding method of anti-edwardsielliasis excellent variety of paralichthys olivaceus

A technique for an excellent variety and flounder, which is applied in the field of artificial breeding of excellent varieties of flounder resistant to Edwardsiosis, can solve the problems of low mortality, low growth rate, complicated breeding methods, etc. The effect of developing market space and less residue

Inactive Publication Date: 2017-10-10
舟山市普陀兴海养殖优质种苗选育研究所
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The flounder seed produced by the technology of the present invention has the characteristics of strong resistance to Edwardsiella tarda, high survival rate, low mortality and fast growth rate, and basically solves the problem of Edwardsiella tarda in summer for the flounder seed. The outbreak of ascites disease caused a large number of deaths, which is suitable for promotion and application in flounder farms in coastal areas of the country, but the breeding method is relatively complicated

Method used

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Examples

Experimental program
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Effect test

Embodiment 1

[0016] An artificial breeding method for an excellent variety of flounder resistant to Edwardsiella, comprising breeding flounder in pond cages, disease prevention, cultivation of Edwardsiella, infection of flounder with Edwardsiella, selection of flounder resistant to Edwardsiella, The specific selection methods are:

[0017] 1) Cage culture of flounder in ponds: choose a pond with a depth of 2.5m to set up cage culture, stock 500 flounder in each box, and arrange the cages in a straight line on the line connecting the inlet and outlet of the pond, which is conducive to smooth water flow and pool water Set the impeller type aerator to fully increase the oxygen and keep the pool water flowing, so as to provide a good environment for the growth of flounder;

[0018] 2) Disease prevention: During the breeding process of flounder, the medicine is administered every 15 days. The composition and weight of the medicine are 12 parts of gallnut, 1.2 parts of protein polypeptide, 16 pa...

Embodiment 2

[0024] An artificial breeding method for an excellent variety of flounder flounder resistant to Edwardsiella, the specific breeding method is:

[0025] 1) Choose a pond with a water depth of 2.2m to set up net cage culture, stock 400 flounder in each box, and arrange the net cages in a straight line on the connection between the inlet and outlet of the pond, which is conducive to the smooth flow of water and the exchange of pool water. The oxygen machine fully increases oxygen and keeps the pool water flowing, providing a good environment for the growth of flounder;

[0026] 2) During the breeding process of flounder, the medicine was administered every 13 days. The ingredients and weight parts of the medicine were 11 parts of gallnut, 1.5 parts of protein polypeptide, 18 parts of forsythia, 15 parts of skullcap, 8 parts of houttuynia, 15 parts of Radix Isatidis, 2 parts of Sophora flavescens, and 2 parts of Golden Cherry Fruit. The amino acid sequence of the protein polypeptide...

Embodiment 3

[0032] An artificial breeding method for an excellent variety of flounder flounder resistant to Edwardsiella, the specific breeding method is:

[0033] Choose a pond with a water depth of 2-3m to set up cage culture, stock 300-500 flounder in each box, and dose the medicine every 13-15 days. 16 parts, 15 parts of Scutellaria baicalensis, 9 parts of Houttuynia cordata, 14 parts of Radix Isatidis, 3 parts of Sophora flavescens, 2 parts of Rosa laevigata; use the strains preserved at -75°C, inoculate directly in the liquid medium under aseptic conditions, Centrifuge the bacteria at 200r / min for 42h at 29°C, centrifuge the bacteria at 8000r / min for 15min, pour off the supernatant, wash with PBS buffer, re-centrifuge and pour off the supernatant, repeat 3 times, and finally dilute to the semi-lethal concentration with PBS buffer ; Select juvenile fish of 10-12 cm from different families with high quality, inject the bacteria solution with a half-lethal concentration intraperitoneal...

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Abstract

The invention discloses an artificial breeding method of an anti-edwardsielliasis excellent variety of paralichthys olivaceus. The method specifically comprises the steps that a pond with the water depth of 2-3 m is selected, and a net cage for breeeding paralichthys olivaceus is arranged; a strain is inoculated into a liquid culture medium for breeding, and centrifuging is conducted; a bacterial solution with the medial lethal concentration of 0.18-0.22 mL / 10g is subjected to intraperitoneal injection for breeding; hybridization, selfing and gynogenesis are used for developing offsprings, the offsprings are bred, and the anti-edwardsielliasis paralichthys olivaceus is obtained. The artificial breeding method has the advantages that the anti-disease variety is bred with the cooperation of family selection and the modern biological technology, which is an important means for solving the paralichthys olivaceus disease problem; a Chinese herbal medicine insecticide is added in the breeding process, an antibacterial effect is achieved on the edwardsiella tarda, residues are few, the drug resistance of pathogenic bacteria is not likely to be caused, the anti-stress capability of organisms can be mobilized, and the immune defense function of the organisms can be improved.

Description

technical field [0001] The invention relates to the field of fish farming, in particular to an artificial breeding method for an excellent variety of flounder that is resistant to Edwardsiella. Background technique [0002] Flounder, teleosts, flounder family. Flounder is commonly known as tooth piece, partial mouth, flounder, flat eye, left mouth, partial mouth fish, flounder, flounder, etc. in China, also known as "left mouth fish". Flounder is a rare marine fish and one of the most important seawater cultured fish. Flounder is a cold-temperate bottom-dwelling fish with sand-diving habits. Most of the juvenile fish live in water depths above 10m, with little organic matter and easy to form eddies. Estuary area. Fattening here in summer. When the water temperature drops in autumn, it will gradually move to the deeper sea area. Generally, it will move to the sea below 50m in September and October, and move south to the seabed with a water depth of 90m or deeper from Novem...

Claims

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Application Information

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IPC IPC(8): A01K61/10A01K61/13
CPCA01K61/10A01K61/13Y02A40/81
Inventor 施晓峰
Owner 舟山市普陀兴海养殖优质种苗选育研究所
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