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Antimicrobial antiphlogistic antagonism polypeptide simulating MD2

An antagonistic, biological polypeptide technology, applied in the direction of anti-inflammatory agents, antibacterial drugs, peptides, etc., can solve problems such as weak affinity and inability to confer TLR4 reactivity

Inactive Publication Date: 2008-08-20
INST OF FIELD OPERATION SURGERY NO 3 MILITARY MEDICL UNIV PLA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Data confirm that the affinity between CD14 and TLR4 / MD2 is weak, while the affinity between TLR4 / MD2 is strong, and the binding of MD2 (myeloid differential protein 2, myeloid differentiation protein 2) to TLR4 (toll like receptor 4, Toll-like receptor 4) Occurs before binding to LPS, and MD2 that has lost its LPS-binding function cannot confer TLR4's responsiveness to LPS even if it binds to the extracellular domain of TLR4

Method used

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  • Antimicrobial antiphlogistic antagonism polypeptide simulating MD2
  • Antimicrobial antiphlogistic antagonism polypeptide simulating MD2
  • Antimicrobial antiphlogistic antagonism polypeptide simulating MD2

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0030] (Completed in six steps)

[0031] Experiment 1. Bioinformatics analysis of MD2 protein and selection of templates for screening antagonistic polypeptides.

[0032] Purpose of the experiment: To use bioinformatics technology to analyze the structural characteristics of MD2 protein, analyze parameters such as hydrophilicity, accessibility, flexibility, etc., and find the key amino acid sequence for the interaction between MD2 and the bacterial toxin LPS. And use this sequence as a template to lay the foundation for subsequent screening of its corresponding antagonistic polypeptides.

[0033] experimental method

[0034]1. Single-parameter prediction: Hydrophilicity, Antigenicity, Accessibility and Flexibility of the main chain of MD2.

[0035] 2. Secondary structure prediction and other information analysis: completed on PHD and SOPMA servers.

[0036] 3. Determination of cell epitopes: Summarize the single-parameter prediction results; exclude sequences whose secondar...

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Abstract

The invention provides an antagonistic peptide which can simulate the key sequence for bonding MD2 and LPS and competitively antagonize the specific bonding which is generated by LPS and MD2, thus achieving the mitigation of the excessive activation of the immune inflammatory cells of LPS and further easing the caused excessive septic damages to the body. The amino acid sequence of the antagonist peptide includes: a sequence 1 of Lys Thr Val Pro Asp Asn His; and the sequence 2 of Ile Gly Lys Phe Leu Tyr Arg. The peptide can effectively mitigate the septic inflammation damage which is induced by the intracellular toxin LPS, thus significantly improving the survival rate of mouses which are infected by the intracellular toxin and maintaining the body immune defense function at the same time of specifically inhibiting the excessive stimulation of the LPS on the inflammatory cells. The antagonistic peptide can be used for preparing the treatment drugs of the inflammatory diseases which are caused by gram-negative bacteria and are related to a toll-like receptor protein.

Description

technical field [0001] The invention belongs to the field of biomedicine, and in particular relates to an antibacterial and anti-inflammatory antagonistic polypeptide simulating MD2, and the preparation of the antagonistic polypeptide is used for the treatment of Gram-negative bacterial infection and related excessive inflammatory damage-like diseases. in the application. Background technique: [0002] Sepsis caused by bacterial infection, especially Gram-negative bacterial infection, has become the main cause of death in clinical patients, especially surgical ICU patients. Data show that the proportion of sepsis in inpatients is 1 / 3, in ICU patients > 50%, and in surgical ICU is more than 80%. Unfortunately, despite the emergence of high-efficiency and broad-spectrum antibiotics, sepsis is still an important cause of death in clinical ICU patients. The main reason is a series of inflammatory damage caused by excessive stimulation of bacterial toxins on the body. Althou...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K7/06A61K38/08A61P29/00A61P31/04
Inventor 李磊闫红胡承香顾长国吴晓华
Owner INST OF FIELD OPERATION SURGERY NO 3 MILITARY MEDICL UNIV PLA
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