The invention discloses a reagent kit for detecting an endometrial cancer susceptibility related genetic marker, and belongs to the technical field of molecular biology and biological medicine. The nucleotide sequence of the genetic marker is as shown in SEQID NO.1, single nucleotide polymorphism that T is greater than G exists in the 501st of the nucleotide sequence, namely that the 501st basic group of the sequence comprises two situations of a basic group T and a basic group G. The reagent kit provided by the invention contains 2 pairs of specific PCR primers designed based on single nucleotide polymorphism of a POU5F1 gene, the single nucleotide polymorphism of the POU5F1 gene is closely related to susceptibility that people suffer from endometrial cancer, through a PCR technique, thesingle nucleotide polymorphism in the POU5F1 gene can be effectively detected, and further the susceptibility of the endometrial cancer is predicted, so that the reagent kit has important clinical significance in screening endometrial cancer susceptible population, detecting high-risk individuals, and performing early prediction, early diagnosis and individualized treatment of the endometrial cancer.