Reagent kit for detecting endometrial cancer susceptibility related genetic marker
An endometrial cancer, genetic marker technology, applied in the direction of microbial determination/examination, biochemical equipment and methods, etc., can solve problems such as early prediction and diagnosis of diseases, and achieve the effect of simple technology, specific detection and high cost performance
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Embodiment 1
[0028] Example 1. Association analysis between detection of POU5F1 gene single nucleotide polymorphism and susceptibility to endometrial cancer
[0029] 1.1 Research object
[0030] The case selection time was from August 2010 to December 2012. From the cases with clear pathological diagnosis in the Obstetrics and Gynecology Hospital Affiliated to Zhejiang University School of Medicine, 272 cases of endometrial cancer were randomly selected, and 346 cases of normal physical examination women were selected at the same time Volunteers were used as normal controls, and the inclusion criteria for normal controls were no other gynecological tumors, no other solid cancers and no immune diseases. All cases and normal control individuals were Han residents. All subjects obtained consent in accordance with the requirements of the Ethics Committee of the Obstetrics and Gynecology Hospital Affiliated to Zhejiang University School of Medicine, and all research methods followed the approv...
Embodiment 2
[0047] Example 2. POU5F1 Gene Single Nucleotide Polymorphism and Endometrial Cancer Susceptibility Detection Kit
[0048] 1. Based on the analysis results of Example 1, we found that the base variation of 501T>G in SEQ ID NO.1 (rs3130932) is significantly related to endometrial cancer, so we designed a specific site for the POU5F1 gene Specific PCR upstream and downstream primers for detection of point (rs3130932) single nucleotide polymorphism, and finally designed a kit for detection of peripheral blood DNA templates of endometrial cancer susceptible population.
[0049] 2. Prepare a special kit (100tests). The specific reagent composition is shown in Table 3, as follows:
[0050] Table 3. Reagent composition of POU5F1 gene single nucleotide polymorphism site detection kit
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[0053] 3. Genomic DNA extraction from peripheral blood cells: extract 1mL of peripheral blood from the subject, use the conventional phenol-chloroform extraction method, or a thi...
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