BRCA gene susceptibility SNP locus detection composition

Abstract

The invention discloses a BRCA gene susceptibility SNP locus detection composition. The BRCA gene susceptibility SNP locus detection composition comprises a primer of SEQ NO:1-SEQ NO:448. The BRCA gene susceptibility SNP locus detection composition has the advantages that more than two thousand susceptibility SNP loci of a BRCA gene can be detected at one time, for the susceptibility SNP loci relative to breast cancer and ovarian cancer, the detection throughput is high, the specificity is strong, the pollution is not prone to occur, and the safety is high, the detection result has good accuracy and repeatability, and certain auxiliary diagnosis and implication effects are achieved for susceptible population of the breast cancer and the ovarian cancer, especially for crowd with family heredity of the two cancers.

Description

Technical field [0001] The invention relates to the field of gene detection, in particular to a composition for detecting the SNPA site of the BRCA gene. Background technique [0002] Breast cancer is one of the most common malignant tumors that seriously affect women's health and even endanger their lives. According to statistics, its incidence accounts for 7-10% of various malignant tumors throughout the body, which is second only to uterine cancer in women. The incidence of breast cancer is often related to genetics. In addition, the incidence of breast cancer is higher in women between 40-60 years of age and before and after menopause. [0003] Breast cancer can be divided into two categories: sporadic and hereditary. Among them, hereditary breast cancer accounts for about 5%-10% of the incidence of breast cancer. Breast cancer susceptibility genes BRCA1 (the first familial breast and ovarian cancer susceptibility gene) and BRCA2 (the second familial breast cancer susceptibi...

AI Technical Summary

Problems solved by technology

[0006] At present, the commonly used BRCA gene mutation detection methods mainly include the following: Fluorescent quantitative PCR technology, which has high sensitivity, specificity, and high degree of automation, but there will be sample contamination and high false positives, and each time only Can detect a type

Restriction small fragment length polymorphism analysis (RFLP) is used to detect genes with altered restriction sites, and can directly determine the genotype, but it cannot be used for the detection of genes without new restriction sites; in addition, the RFLP experimental operation Complicated, long detection cycle, high cost, false positive, non-closed tube operation, easy to contamination, difficult to meet clinical detection requirements

In the first-generation DNA sequencing method, the sequences close to the primers at both ends are easy to be inaccurate, and the sequencing cycle is long, the operation is complicated, easy to be polluted, and the cost is high, so this method is difficult to meet the needs of practical applications

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