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IL-6 gene SNP marker related to chronic hepatitis b virus (HBV) infection and application thereof

A chronic hepatitis B, hepatitis B technology, applied in the application field of genetic counseling, can solve problems such as affecting liver damage and disease severity, changing expression or binding activity, etc.

Active Publication Date: 2016-05-18
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Polymorphisms in this gene may alter its expression or binding activity, thereby affecting liver damage and disease severity

Method used

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  • IL-6 gene SNP marker related to chronic hepatitis b virus (HBV) infection and application thereof
  • IL-6 gene SNP marker related to chronic hepatitis b virus (HBV) infection and application thereof
  • IL-6 gene SNP marker related to chronic hepatitis b virus (HBV) infection and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] This embodiment adopts a method for detecting the SNP site of IL-6 gene related to the outcome of chronic hepatitis B.

[0024] 1. Genomic DNA extraction

[0025] Whole blood DNA was extracted using the QIAampDNABloodMini kit from Qiagen, USA, and according to the instructions.

[0026] Preparation before the experiment: transfer the blood sample to a 4°C refrigerator one day in advance to allow the blood to thaw. Turn on the metal bath and adjust it to 56°C, prepare centrifuge tubes, masks, napkins, kitchen paper, alcohol spray cans, waste paper baskets, waste liquid tanks, etc. (refer to qiagen kit)

[0027] 1. Add 20ul protease to a 1.5ml centrifuge tube; if RNA needs to be removed, add 4ul RNAse to the tube.

[0028] 2. Add 200ul sample to the 1.5ml centrifuge tube; please do not touch the tube wall when drawing blood, so as not to contaminate the pipette.

[0029] 3. Add 200ul buffer solution AL, mix thoroughly by inverting, and shake for 15s.

[0030] 4.56 ℃ ...

Embodiment 2

[0044] This embodiment uses the method of embodiment 1, to 191 routine hepatitis B patients, 177 routine hepatitis B liver cirrhosis patients, 48 ​​routine hepatitis B liver failure patients, 93 routine HBsAg and HBeAg all negative carriers and 112 cases The genotypes and alleles of IL-6 gene rs2066992 and rs1524107 loci of healthy subjects in the International Health Examination Center were analyzed.

[0045] 1. Test object

[0046] During the period from November 2013 to November 2015, this study collected 93 HBsAg and HBeAg negative carriers, 191 chronic hepatitis B patients, 177 cases of hepatitis B cirrhosis patients, 48 ​​cases of hepatitis B liver failure patients and 112 healthy checkups from the International Health Examination Center, a total of 621 cases.

[0047] Patient Inclusion Criteria

[0048] Case diagnosis was carried out according to the case judgment criteria stipulated in the 2015 edition of "Guidelines for the Prevention and Treatment of Chronic Hepati...

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Abstract

The invention relates to the individual susceptibility of the disease course progress of a chronic hepatitis b virus (HBV) infected person, and particularly relates to an application of a susceptible gene IL-6 SNP marker related to the disease course process progress of the HBV chronic infected person in early warning and genetic counseling of the outcome of the HBV chronic infected person. The invention provides an IL-6 gene SNP marker related to the outcome of chronic HBV and particularly related to the outcome of HBV-related hepatocirrhosis and a detection reagent and a detection method. For the chronic HBV patients having an rs2066992-site TT gene or a T allele or an rs1524107-site TT gene, scientific interference can be performed, for example, the patient is advised to take the curative prevention measures as early as possible, so that the risk of HBV-related hepatocirrhosis of susceptible people can be reduced on purpose.

Description

technical field [0001] The present invention relates to the individual susceptibility of chronic hepatitis B virus (HepatitisBvirus, HBV) infection to progress of disease course. Specifically, the present invention relates to the application of the SNP marker of the susceptibility gene IL-6 related to the progress of the HBV chronic infection in the early warning of the disease outcome of the HBV chronic infection and genetic counseling. Background technique [0002] According to the updated data of WHO in 2015, there are about 240 million people with chronic hepatitis B virus (HBV) infection worldwide. Worldwide, more than 780,000 people die from various complications of hepatitis B every year, including liver cirrhosis and liver cancer. my country is a high-incidence area of ​​viral hepatitis. There are about 93 million chronic HBV infected people, including about 20 million chronic hepatitis B (CHB) patients. Generally, chronic hepatitis B develops gradually and causes ...

Claims

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Application Information

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IPC IPC(8): C12Q1/68C12N15/11
CPCC12Q1/6883C12Q2600/118C12Q2600/156
Inventor 郑敏陈智夏彩霞刘艳宁陈峰黄春红楼国华朱伟
Owner ZHEJIANG UNIV
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