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SNP (single nucleotide polymorphisms) associated with cervical cancer and method for detecting susceptible populations of cervical cancer

A technology for susceptible populations and cervical cancer, applied in the fields of gynecological oncology and molecular genetics, can solve problems such as the lack of comprehensive, systematic and detailed research results

Active Publication Date: 2013-10-23
TONGJI HOSPITAL ATTACHED TO TONGJI MEDICAL COLLEGE HUAZHONG SCI TECH
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Problems solved by technology

Although research on susceptibility genes for cervical cancer has been carried out for many years at home and abroad, comprehensive, systematic and detailed research results have not yet been obtained.

Method used

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  • SNP (single nucleotide polymorphisms) associated with cervical cancer and method for detecting susceptible populations of cervical cancer
  • SNP (single nucleotide polymorphisms) associated with cervical cancer and method for detecting susceptible populations of cervical cancer
  • SNP (single nucleotide polymorphisms) associated with cervical cancer and method for detecting susceptible populations of cervical cancer

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Embodiment 1

[0028] Next, the allelic differences of rs13117307 on chromosome 4q12, rs4282438, rs9277952 on chromosome 6p21.32, and rs8067378 on chromosome 17q12 were detected by TaqMan assays (Applied Biosystems, USA) to determine the susceptible population for cervical cancer.

[0029] 1. Genomic DNA Extraction

[0030] All subjects were drawn 5ml of anticoagulated blood from peripheral vein. Genomic DNA was extracted with a Whole Blood DNA Extraction Kit (QuickGene) produced by Fujifilm, Japan. The steps are as follows: (1) Take 250ul of cell lysate (LDB) and 30ul of protease solution and add them to the marked 1.5ml centrifuge tube, take 200ul of blood sample into the centrifuge tube, repeatedly pipette until the mixture is uniform, and shake on the shaker for 15 seconds; (2 ) in a water bath at 56°C for 3 minutes. During the water bath, keep about 1 / 3 of the nozzle on the liquid surface to prevent the cap from opening due to heating and impurities such as water entering the tube; (3)...

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Abstract

The invention relates to SNP (single nucleotide polymorphisms) associated with a cervical cancer, provides a primer and a probe for detecting susceptible populations of the cervical cancer by using the SNP, and also provides a method for detecting the susceptible populations of the cervical cancer by using the SNP, the primer and the probe. The method is achieved by the following steps: genome DNA (deoxyribonucleic acid) extraction; Taqman PCR (polymerase chain reaction) augmentation of a target segment containing SNP sites; fluorescence collection and genetic typing of a PCR product; and result judgment.

Description

technical field [0001] The invention relates to the fields of gynecological oncology and molecular genetics, in particular, the invention relates to SNPs associated with the occurrence of cervical cancer and a method for using these SNPs to detect cervical cancer susceptible population. Background technique [0002] Cervical cancer is one of the malignant tumors that endanger women's health, and its mortality rate accounts for the third place among female malignant tumor diseases worldwide. 1 . About 500,000 new cervical cancer patients are diagnosed every year in the world, and more than 268,000 people die of cervical cancer every year 2 , while 80% of the above cases occurred in developing countries 3 . The morbidity and mortality of cervical cancer in China ranks second in the world 3 . At present, the incidence of cervical cancer is on the rise in some areas, and the patients tend to be younger. With the development of social economy, cervical cancer, a malignant d...

Claims

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Application Information

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IPC IPC(8): C12N15/11C12Q1/68
Inventor 马丁王世宣周剑峰李双邓东锐
Owner TONGJI HOSPITAL ATTACHED TO TONGJI MEDICAL COLLEGE HUAZHONG SCI TECH
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