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Single nucleotide polymorphism site relevant to susceptibility of prostate cancer and application of single nucleotide polymorphism site

A single nucleotide polymorphism, prostate cancer technology, applied in the direction of DNA / RNA fragment, recombinant DNA technology, etc., can solve the problem of no prostate cancer related reports

Inactive Publication Date: 2013-03-27
SHANGHAI CHANGHAI HOSPITAL +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

After searching the literature at home and abroad, there is no report on the association between the 9q31.2 genetic region and prostate cancer, and there is no report on the correlation between the polymorphic site in the 9q31.2 region and prostate cancer.

Method used

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  • Single nucleotide polymorphism site relevant to susceptibility of prostate cancer and application of single nucleotide polymorphism site
  • Single nucleotide polymorphism site relevant to susceptibility of prostate cancer and application of single nucleotide polymorphism site
  • Single nucleotide polymorphism site relevant to susceptibility of prostate cancer and application of single nucleotide polymorphism site

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Experimental program
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Effect test

Embodiment 1

[0014] The genotyping of the single nucleotide polymorphism rs817826 was performed by extracting the subject's genomic DNA, and the individuals whose rs817826 base was C were the susceptible population of prostate cancer. The specific method is:

[0015] 1. Extraction of Genomic DNA

[0016] All subjects were drawn 2-3ml of peripheral venous blood, and genomic DNA was extracted with QIAamp Blood Mini Kit250 (QIAGEN, Germany) kit, and stored at -20°C.

[0017] 2. SNP genotyping

[0018] According to the sequence information of the SNP, PCR reactions and single-base extension primers were designed using Sequenom’s primer design software MassARRAY Assay Design 3.1 (Sequenom, Inc., San Diego, CA), and synthesized at Bioengineering (Shanghai) Co., Ltd. The synthesized primers were subjected to PCR reaction with the sample DNA, and the mixed reaction products were subjected to SNP genotyping on the Sequenom iPLEX instrument. The specific experimental process is as follows:

[00...

Embodiment ２

[0043] Correlation between SNP in 9q31.2 genetic region and prostate cancer

[0044] In this study, through a multi-stage case-control study, it was shown that the SNP in the 9q31.2 genetic region was associated with prostate cancer. The study took 4484 cases of prostate cancer patients as the research group, and 8934 cases of non-tumor patients as the control group. Genomic DNA was extracted from all subjects and stored at -20°C.

[0045] The results of the multi-stage case-control study showed that those whose rs817826 base is C have a high risk of prostate cancer, as shown in the following table:

[0046]

[0047] a TT / TC / CC

[0048] Examples of the utility of the invention:

[0049] (1) According to the method of the present invention for determining the susceptible population of prostate cancer by the polymorphic site rs817826, the

[0050] Screening of people without clinical symptoms of tumors can be used for auxiliary diagnosis of prostate cancer and judgment ...

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Abstract

The invention belongs to the field of genes of a biological technology, and in particular to a single nucleotide polymorphism site relevant to susceptibility of prostate cancer and application of the single nucleotide polymorphism site. The invention provides sequences of partial genes in a genetic region 9q31.2 relevant to the susceptibility of the prostate cancer, and nucleotide sequences are shown as SEQ.ID.NO.1. The invention also provides a single nucleotide polymorphism site rs817826 on the genetic region 9q31.2, wherein individuals with rs817826 basic groups as C are prostate cancer susceptible population, individuals with rs817826 basic groups as T are prostate cancer non-susceptible population; and an SNP (Single Nucleotide Polymorphism) sequence of the rs817826 is shown in SEQ.ID.NO.2. The invention also provides specific nucleic acid primers, which are shown in SEQ.ID.NO.3, SEQ.ID.NO4 and SEQ.ID.NO.5, of the single nucleotide polymorphism site, wherein amplified products of the single nucleotide polymorphism site are specifically amplified.

Description

technical field [0001] The invention belongs to the gene field of biotechnology, and specifically relates to a single nucleotide polymorphism site related to prostate cancer susceptibility and application thereof. Background technique [0002] Prostate cancer (PCa) is the second most common tumor in men and the sixth leading cause of cancer-related death. It is estimated that there will be 914,000 new cases and 258,000 deaths worldwide each year (Jemal A, Bray F , Center MM, Ferlay J, Ward E, Forman D. Global cancer statistics. CA Cancer J Clin 2011;61, 69-90.). Most of the etiology of prostate cancer is not very clear, the current research results show that, in addition to age and race, family history is another major risk factor for prostate cancer (Gronberg H. Prostate cancer epidemiology. Lancet. 2003; 361 (9360): 859-64.). This familial aggregation largely suggests the influence of genetic factors on the risk of prostate cancer. In a twin study, genetic factors expla...

Claims

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Application Information

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IPC IPC(8): C12N15/11C12Q1/68
Inventor 孙颖浩徐剑锋莫曾南许传亮任善成高旭
Owner SHANGHAI CHANGHAI HOSPITAL
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