Detection tool for screening susceptible population of bird influenza and human influenza viruses
A technology for human influenza virus and susceptible populations, which is applied in the field of screening avian influenza and human influenza virus susceptible populations to achieve the effects of highly targeted testing and low production costs
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[0040] Whole saliva sample collection: male patients aged 45-65 with hepatitis B, 17 cases of liver cirrhosis and patients with liver cancer; 26 cases of male and female patients with type 2 diabetes aged 50-65; men with gastric cancer aged 50-65 27 patients and 30 healthy males and females aged 45-65 years old, two hours after meals, between 9:00 and 10:00, rinsed with normal saline for three times and quickly collected the whole saliva secreted naturally. At least 1 mL of saliva was collected and immediately placed on ice, and protease inhibitors (10 μL per mL of saliva) were added to prevent protein degradation.
[0041] The main experimental steps are:
[0042] 1) Preparation of lectin chips;
[0043] 1.1) Take the epoxy film base for use;
[0044] 1.2) Prepare the lectin into a sample solution with a concentration of 1mg / mL;
[0045] 1.3) Add the prepared spotting solution into the 384-well plate; then use the crystal core 48 spotting system to spot the sample on the e...
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