The invention discloses a competitive
enzyme-linked immunosorbent
assay method of an EV71
neutralizing antibody, a kit or a
reagent and a preparation method thereof. The kit utilizes the labeled
monoclonal antibody which has higher neutralizing
potency on three genes of A, B and C of EV71
virus, a reference material of the EV71
neutralizing antibody and a coated plate and can simply, rapidly and accurately carry out qualitative and
quantitative assay on the content of the EV71
neutralizing antibody in a human sample or an animal sample. EV71
virus particles are coated on the ELISA plate, the
enzyme-labeled EV71 neutralizing
antibody is diluted according to a certain proportion, then respectively mixed with the sample to be assayed and the reference material of the EV71 neutralizing
antibody and reacted with an EV71
antigen coated on the ELISA plate, a semi-logarithmic
standard curve is drawn according to the
absorptance percentage of OD value of the reference
serum reaction and the logarithm of the
potency of the reference material of the EV71 neutralizing antibody after color development, and the
absorptance percentage of the OD value of the sample to be assayed is substituted ina
standard curve equation to calculate the corresponding neutralizing
potency.