62results about How to "Accurate quantitative determination" patented technology

The invention provides a method for detecting the content of impurity elements in electrolyte of a vanadium cell. The method comprises the following steps: measuring primary sample solution of electrolyte of the vanadium cell as per the proportional relation of dilution by 5 to 10 times, placing the primary sample solution in a measuring flask, adding water for dilution to fix volume, adding standard solution of elements to be detected with different volumes in prepared matrix matching base solution formed by vanadium ion, hydrogen ion and sulfate ion, finally preparing standard solution of elements to be detected with different concentration gradients, drawing a standard curve of standard solution of elements to be detected, inspecting linearly dependent coefficients, determining the primary sample solution, which is prepared through dilution, of electrolyte of the vanadium cell, , multiplying a detection result by the dilution ratio to obtain the content of impurity elements in electrolyte of the vanadium cell. The method has good precision and reproducibility of preparation of sample solution and instrument measurement.

The invention discloses a quantitative detection method of polysaccharide containing uronic acid. The method comprises the following steps: preparing a standard polysaccharide solution and a sample solution to be tested; hydrolyzing polysaccharide in the standard solution and the sample solution to be tested so as to obtain hydrolyzed residue; derivatizing the residue with 1-phenyl-3-methyl-5-pyrazolone to prepare a sugar derivative; carrying out chromatographic analysis on the sugar derivative by the utilization of a high performance liquid chromatograph and a triple quadrupole linear ion trap mass spectrometer; determining which kind of polysaccharide containing uronic acid the sample to be tested is according to the chromatogram; and preparing a standard curve of chromatographic peak area and polysaccharide concentration according to the chromatogram of the standard polysaccharide disaccharide derivative obtained by high performance liquid chromatography-mass spectrum, and calculating content of polysaccharide containing uronic acid in the sample to be tested by the utilization of peak area of the sample to be tested. According to the method provided by the invention, quantitative detection of polysaccharides can be accurately carried out, and effective means is provided for quality control of products such as medicines, health product and the like of the polysaccharides.

Electrochemical biosensor test strip, fabrication method thereof and electrochemical biosensor are disclosed. The electrochemical biosensor test strip is fabricated by cutting a groove in a first insulation base in the breadth direction, forming two electrodes parallel to length direction on the first insulation base by sputtering using shadow mask, fixing a reaction material comprising an enzyme which reacts an analyte and generates current corresponding to the concentration of analyte across the two electrodes on the groove of the insulation base, and affixing a cover to the first insulation base. The groove of the first insulation base and the cover make a capillary at the position where the reaction material is fixed. The fabrication method can lower the cost for fabricating the test strip by forming thin electrodes.

There is provided a fluorescent X-ray analysis apparatus in which a detection lower limit is improved, and it is possible to quantify a trace aimed element having been contained not only in a sample whose main component is a heavy element but also in a sample whose main component is a light element. The fluorescent X-ray analysis apparatus possesses a sample base supporting the sample, an X-ray source irradiating a primary X-ray with a predetermined irradiation position being made a center, and a detector disposed toward the irradiation position and detecting a fluorescent X-ray generated from the sample. The sample base has a detachable sample holding tool fixing the sample while being approached to the X-ray source and the detector, and a measurement is possible by selectively disposing the sample in any one of a 1st inspection position in which an irradiated face is coincided with the irradiation position, or a 2nd inspection position in which the sample is fixed to the sample holding tool, an irradiated face is approached to the X-ray source, and an inspected face is approached to the detector.

The invention discloses a quality control method for edaravone and an edaravone-containing preparation. In the method, an impurity, namely, 2,2',4,4'-tetrahydrophthalic anhydride-5,5'-dimethyl-2,2'-diphenyl-[4,4'-di-3H-parazole]-3,3'-dione in the edaravone and an edaravone preparation is determined quantitatively by photoelectric technology. By the technical scheme of the invention, the qualities of the edaravone and an edaravone-containing medicament preparation can be evaluated and controlled more reliably and accurately.

The invention belongs to the field of pharmaceutical analytical chemistry, and particularly relates to two methods for analyzing and measuring potential genotoxic impurities in pantoprazole sodium anda synthetic starting material 5-difluoromethoxy-2-mercapto-1H-benzimidazole thereof. According to the method, octadecylsilane chemically bonded silica is used as a stationary phase, and a mass spectrometry detector is used for analysis and determination; the mobile phase is an aqueous solution containing acetonitrile, methanol and organic ammonium salt; the structural types of the potential genotoxic impurities comprise halogenated methylpyridine compounds, azaaryl N-oxides, N-acylated aminobenzene compounds, aromatic amines compounds and nitrobenzene compounds. According to the method, the effective separation of potential genotoxic impurities in pantoprazole sodium and synthesis starting raw materials of pantoprazole sodium can be realized, the genotoxic impurities can be accurately andquantitatively determined; the method is high in sensitivity and specificity; the experimental operation is simple, convenient and rapid, and the method has important significance for quality controland medication safety of pantoprazole sodium.

The invention provides an analysis system and an analysis method of two-dimensional energy associated laser induced breakdown spectroscopy. The analysis system comprises a laser, a spectrograph, a delay generator, an optical fiber, a sample and a computer, wherein the laser is used for periodically transmitting pulse laser to strike the sample; the delay generator is respectively connected with the laser and the spectrograph and used for triggering a spectrograph acquisition signal according to a preset delay time; the spectrograph is respectively connected with the optical fiber and the computer and used for acquiring a plasma optical signal transmitted by the sample by virtue of the optical fiber. According to the analysis system and the analysis method of the two-dimensional energy associated laser induced breakdown spectroscopy, the conventional laser induced breakdown spectroscopy is carried out on a laser energy scale, so that the spectral signature can be clearly analyzed, and the detection capability and the repeatability of the conventional laser induced breakdown spectroscopy method can be improved.

A positive-pressure automatic spume proportionality mixer systems includes pressure water pipeline, spume pipeline and mixer pipeline. There are at least spume tank, spume purification apparatus, ram-type pump, spume application valve ball and back valve setup at the spume pipeline with spume tank at the beginning, the out spout pipeline of back valve joins with pressure water pipeline to mixer pipeline. A flow rate sensor is set at the aforementioned mixer pipeline and connects to control module with wires, the control module connects with parameter input and display module and machine controller connecting with electric motor drive ram-type pump. The system can implement whole automatic control spume mixing proportion with simple operation, accurate adjustment, stabilizing and reliable operation activity.

The invention relates to a content determination method for scutellarin and scutellarein in sculellaria barbata medicinal material and formula granule of sculellaria barbata medicinal material. The content determination method is characterized in that common isocratic elution is adopted, acetonitrile-methyl alcohol-0.1 percent phosphoric acid of which the volume ratio is 12.5:(15 to 16.2):(71.3 to 72.5) is taken as a mobile phase, and the contents of the scutellarin and the scutellarein in the sculellaria barbata medicinal material and the formula granule of the sculellaria barbata medicinal material are simultaneously determined at a wavelength of 340+ / -2nm. The method is simple, convenient, fast, and easy to popularize and master; the separations of wave crests on three different chromatographic columns are good. Compared with a method of recording under a sculellaria barbata medicinal material item of the first part of the pharmacopeia, the prominent improvement is that a sample treatment process is simplified, the time and cost are saved, the determination is advanced from single component determination of the scutellarin to the simultaneous determination of the two components, the quality controllability is improved, and the defects that impurity peaks exist and the content results are inaccurate are overcome.

The invention discloses a fluorescence quantitative PCR detection method for type and composition of yak muscle fibers. According to the fluorescence quantitative PCR detection method, yak muscle tissues are taken for total RNA extraction, the RNA purity and concentration are detected, the total RNA is subjected to inverse transcription to generate cDNA, then quality detection is carried out by taking GAPDH genes of yak as internal genes, and then a special detection primer is adopted for carrying out fluorescence quantitative PCR reaction, meanwhile, the GAPDH genes of yak are taken as reference genes for statistic analysis. The detection method and the detection primer provided by the invention are more accurate and more reliable than histochemistry evaluation; with the molecule quantitative classification method, accurate quantitative and analysis can be carried out on the type composition of the various muscle fibers in muscles at different parts of the yak, so that a technological base is laid for further study on the relevance between the muscle fibers and the meat quality trait of the yak, and meanwhile, compared with other methods, the fluorescence quantitative PCR detection method has the advantages of low workload and low cost, and high efficiency.

A container and method for taking out contained product. The container comprises a container body, a pressure transfer channel, a control valve, a liquid taking channel and an outflow channel, the control valve contains a moving part. The moving part is at different positions along with variation of pressure applied to a variable pressure part, and connection and disconnection of the channels which between the pressure transfer channel and the outflow channel and between the liquid taking channel and the pressure transfer channel are determined by three positions of the moving part. The container can quantitatively take out contained product, is simple in structure and avoid the product to be oxidized or polluted.

The invention relates to the field of textile testing and discloses a method for tracing silk production places on the basis of a stable isotope technique. The stable isotope technique is adopted for tracing the silk production places by testing isotopic values of hydrogen, oxygen, carbon and nitrogen and strontium in mulberry leaves, mulberry twigs, silkworm cocoons and soil and isotopic values of hydrogen and oxygen in water samples, results are finally subjected to clustering analysis, and the silk production places are traced. Transfer and transformation of metabolites can be accurately and quantitatively determined by the aid of the stable isotope technique, and the silk production places can be scientifically and stably traced.

The invention relates to a fingerprint resistant film, in particular to a method for detecting the weight of the fingerprint resistant film. The method for detecting the weight of the fingerprint resistant film of a fingerprint-resistant electro-galvanized sheet comprises the following steps of: preparing dezincing solution and zinc standard solution; sampling; sample processing; detecting the total weight of a galvanized coating; detecting galvanized amount; and working out the weight of the fingerprint resistant film. By the method, the weight of the fingerprint resistant film can be directly, correctly and quickly detected, and the detection is not influenced by the change of fingerprint resistant raw material components and a production process.

The invention discloses a detection method of xylooligosaccharide. According to the method, a high-performance liquid chromatograph and an evaporative light scattering detector are used for performing qualitative and quantitative detection on small polymers, with different degrees of polymerization, in the xylooligosaccharide. The method is very high in detection sensitivity and can quantitatively detect the small polymer components in the xylooligosaccharide accurately; the separating effect is good; and the detecting repeatability is good.

The present invention provides genetic marker, method and reagent kit for detecting ox originated / sheep originated components in feed. Specifically, the present invention provides the D-loop doze nucleotide sequence of the ox originated / sheep originated components in feed and the primer and probe designed based on the sequence. The method may be used in convenient, fast and accurate qualitative and quantitative detection of ox originated / sheep originated components in feed.

The invention provides a method for quantitative determination of uronic acid-containing polysaccharide. The method comprises the following specific steps of preparing a uronic acid-containing polysaccharide standard substance solution from trifluoroacetic acid, wherein the uronic acid-containing polysaccharide is the polysaccharide of which a main chain is formed by a hexuronic acid-hexosamine or hexuronic acid-hexose disaccharide repeated disaccharide fragment; carrying out acid hydrolysis and deacidification on a standard substance to obtain a to-be-tested sample acid hydrolysis residue; dissolving a standard sample acid hydrolysis residue, and carrying out analysis by using a high-performance liquid chromatography-tandem triple quadrupole mass spectrometer to obtain a multi-response monitoring chromatogram and a standard curve; processing and detecting the to-be-tested sample acid hydrolysis residue by adopting the same method; and identifying the variety of the uronic acid-containing polysaccharide and obtaining the content of the uronic acid-containing polysaccharide in the to-be-tested sample. The method is simple in operation step, low in reagent price and relatively short in elapsed time, and polysaccharide formed by repeated disaccharide fragments of uronic acid and hexosamine or hexose can be accurately quantified at the same time.

A positive-pressure automatic spume proportionality mixer systems includes pressure water pipeline, spume pipeline and mixer pipeline. There are at least spume tank, spume purification apparatus, ram-type pump, spume application valve ball and back valve setup at the spume pipeline with spume tank at the beginning, the out spout pipeline of back valve joins with pressure water pipeline to mixer pipeline. A flow rate sensor is set at the aforementioned mixer pipeline and connects to control module with wires, the control module connects with parameter input and display module and machine controller connecting with electric motor drive ram-type pump. The system can implement whole automatic control spume mixing proportion with simple operation, accurate adjustment, stabilizing and reliable operation activity.

The invention discloses a device for simulating emission of greenhouse gas of a drainage channel under an intermittent hydrodynamic condition and an experimental method thereof. The device comprises arice field system, a channel water tank, a water-bottom mud collecting system, a gas collecting system, a water supply system, a tail door and a water discharging groove; the water supply system is located on the side surface of the rice field system and is connected through a water channel pipeline; a rectangular plastic basin bowl in the rice field system is connected with the channel water tank at one side of a field ridge; the rice field system is used for realizing irrigation through the water supply system; water discharge to the channel water tank is realized through the field ridge; the gas collecting system and the water-bottom mud collecting system are arranged in the channel water tank at equal distances; the tail door is arranged at the tail part of the water tank, and the back part of the tail door is connected with a drainage groove; the greenhouse gases with different space depths in the drainage channel and the water and the bottom mud are sampled together in the controllable intermittent hydrodynamic condition, and the device has an important theory and practical significance for accurately measuring the emission flux and concentration distribution of greenhouse gas in the drainage channel of the rice field, disclosing the influence mechanism and the control factors.

The invention discloses pazufloxacin mesilate and a quality control method of an injection preparation. According to the method, high performance liquid chromatography is employed for performing quantitative determination on pazufloxacin mesilate and an impurity pazufloxacin mesilate dextroisomer in the preparation. The technical scheme provided by the invention is capable of relatively reliably and relatively accurately evaluating and controlling the quality of pazufloxacin mesilate and the injection preparation.

The invention discloses a living body spontaneous activity quantitative detection device which comprises a box body structure with an upper layer and a lower layer, wherein the upper layer is a sensor layer which comprises infrared geminate transistors arranged in a 16*8 matrix, spacing between every two adjacent infrared geminate transistors is 3cm, infrared ray transmitting tubes and infrared ray receiving tubes of the infrared geminate transistors are arranged in parallel, a transparent plate is arranged above the infrared geminate transistors, the lower layer is a control plate layer which comprises a scanning circuit and a control circuit, the scanning circuit and the control circuit are electronically connected, the scanning circuit comprises 18 pieces of CD4051, the control circuit mainly comprises an ATMEGA16 processor chip, the infrared geminate transistors is connected with the scanning circuit through a transmission line, and the control circuit is connected with a display module outside the box body structure. The invention further discloses a detection method with the device used. According to the method, automatic detection of the whole process of the activity of a mouse can be achieved comprehensively and accurately with a simple method, accurate reflection and continuous detection of the process of the activity of the mouse are achieved, and accurate quantitative description of activity in two-dimensional space is achieved.

The embodiment of the invention provides a method and device for determining the porosity of cracks. The method comprises the following steps: acquiring an outcrop sample and a rock core sample in a target region; carrying out three-dimensional laser scanning on the outcrop sample, and establishing a reservoir geologic model of the target region; determining extension lengths of the cracks according to the reservoir geologic model of the target region; determining the opening, filling coefficients and penetration coefficients of the cracks according to the rock core sample; and determining the porosity of the cracks in the target region according to the extension lengths, opening, filling coefficients and penetration coefficients of the cracks. According to the scheme, the reservoir geologic model is established by virtue of the outcrop sample with a relatively good representation effect and is used for quantitatively determining the cracks; by combining with rock core data, the porosity of the cracks is accurately determined, so that the technical problems that the precision of the determined porosity of the cracks is low, and the accuracy is relatively poor in existing methods are solved, and the porosity of the cracks is rapidly, accurately and quantitatively determined.

The present invention provides, among other things, methods of quantitating mRNA capping efficiency, particularly mRNA synthesized in vitro. In some embodiments, methods according to the present invention comprise providing an mRNA sample containing capped and uncapped mRNA, providing a cap specific binding substance under conditions that permit the formation of a complex between the cap specific binding substance and the capped mRNA, and quantitatively determining the amount of the complex as compared to a control, thereby quantifying mRNA capping efficiency.

The invention discloses a method for determining the minimal residual lesion level based on multiple amplification sequencing. Comprising the following steps: S1, sample treatment, S2, primer pool manufacturing, S3, single-ended amplification, S4, quality control, S5, purification, S6, mixed sequencing, S7, sequencing data analysis, and S8, minimal residual lesion level calculation. According to the determination method, simple and convenient single-ended amplification is used, the minimal residual lesion level can be accurately and quantitatively detected, an external reference is not needed, meanwhile, primer dimers can be prevented from being generated, the proportion of effective data is increased, and then the accuracy of determining the MRD level is improved.

The invention provides a method for detecting genetic toxic impurities of SM3 (4-amino-1-methyl-3-n-propylpyrazole-5-formamide) in a class 1 new drug aldenafil citrate bulk drug. A sample to be detected is detected by using a high performance liquid chromatography-mass spectrometry tandem method. According to the method provided by the invention, the genotoxic impurity 4-amino-1-methyl-3-n-propylpyrazole-5-formamide in the Adenafil can be effectively determined with ultrahigh sensitivity, so that strict quality control is implemented.

The invention belongs to the technical field of chromatographic detection, and specifically relates to a method for quickly detecting vancomycin by utilizing LC-MS (Liquid Chromatography-Mass Spectrometry). The method comprises the following steps: smashing a to-be-detected sample, adding an extracting reagent, and uniformly mixing; then carrying out ultrasonic extraction, and centrifuging, thus obtaining supernatant; extracting the supernatant by adopting water saturated normal hexane, thus obtaining a to-be-purified solution; activating through an MCX solid-phase extraction column, taking and sampling the to-be-purified solution, carrying out drip washing and impurity removing by using methanol, then eluting by using amonium methanol, and collecting an eluent; concentrating, forming a constant volume by using a constant volume solution, and filtering, thus obtaining a purified solution; detecting the purified solution by adopting an HPLC-MS (High Performance Liquid Chromatograph-MassSpectrometer). The method disclosed by the invention has the advantages that the problems of large matrix effects, low sensitivity and the like existing in a process of detecting animal derived foodand particularly the animal derived food with a high fat content in the prior art are well solved, ideal recovery and precision degree are obtained, the minimum detection limit of the method is 0.3 mug / kg, and repeatability and reproducibility of an analysis result are effectively ensured.

The invention provides a method for quantitatively measuring the rusting ratio of the surface of a metal material, and the method includes the following steps: obtaining a local or total surface image of a to-be-measured sample through image acquisition equipment and inputting the image in a computer; opening the surface image of the to-be-measured image through phase area content measuring software of an automatic metallographical image analyzer; selecting a regional image required for measurement in the surface image of the to-be-measured sample; performing binaryzation treatment on the regional image, so as to convert the regional image into a two-value image only containing two colors; converting colors of all the rust regions in the regional image into one color of the two colors through adjusting the range of the threshold; extracting regions with the color representing the rust regions in the two-value image of the regional image; and calculating the percentage of the total area of the regions with the color representing the rust regions and the area of the regional image through the content measuring function of the phase area content measuring software, so as to obtain the rusting ratio of the surface of the to-be-measured sample. The method provided by the invention can be used for accurately measuring the rusting ratio of the surface of the metal material sample, and is simple, convenient and quick.

The invention discloses a separation and determination method for the mobility of nicotine optical isomers in cigarettes. The method comprises the following steps of: (1) collecting nicotine optical isomers in cigarettes; 2, collecting nicotine optical isomers in cigarette aerosol; (3) carrying out accurate quantitative determination on S-(-)-nicotine and R-(+)-nicotine; and (4) calculating the mobility of the nicotine optical isomers in the cigarettes. According to the separation and determination method, a Trap column is adopted for the first time to replace a quantitative loop, so that a target object cut by a one-dimensional column can be completely collected, and quantitative determination is more accurate; it is found for the first time that the mobility of R-(+)-nicotine to aerosolin traditional cigarettes is obviously higher than that of R-(+)-nicotine to aerosol in heated cigarettes.

The invention belongs to the technical field of detection, and particularly relates to a detection method for simultaneously determining the content of eight compounds in glipizide drugs. The determination method is a reversed-phase high performance liquid chromatography method. The method can be used for simultaneously determining eight compounds in glipizide synthetic intermediates, glipizide bulk drugs and glipizide-containing drugs.

Methods for quantitatively determining a binding kinetic parameter of a molecular binding interaction, for example wherein the determination involves a complex sample, are provided. Aspects of embodiments of the methods include: producing a magnetic sensor device including a complex sample including a magnetic sensor in contact with an assay mixture including a magnetically labeled molecule to produce a detectable molecular binding interaction; obtaining a real-time signal from the magnetic sensor; and quantitatively determining a binding kinetics parameter of the molecular binding interaction from the real-time signal. Also provided are systems and kits configured for use in the methods.