62results about How to "Accurate quantitative determination" patented technology

The invention discloses a quantitative detection method of polysaccharide containing uronic acid. The method comprises the following steps: preparing a standard polysaccharide solution and a sample solution to be tested; hydrolyzing polysaccharide in the standard solution and the sample solution to be tested so as to obtain hydrolyzed residue; derivatizing the residue with 1-phenyl-3-methyl-5-pyrazolone to prepare a sugar derivative; carrying out chromatographic analysis on the sugar derivative by the utilization of a high performance liquid chromatograph and a triple quadrupole linear ion trap mass spectrometer; determining which kind of polysaccharide containing uronic acid the sample to be tested is according to the chromatogram; and preparing a standard curve of chromatographic peak area and polysaccharide concentration according to the chromatogram of the standard polysaccharide disaccharide derivative obtained by high performance liquid chromatography-mass spectrum, and calculating content of polysaccharide containing uronic acid in the sample to be tested by the utilization of peak area of the sample to be tested. According to the method provided by the invention, quantitative detection of polysaccharides can be accurately carried out, and effective means is provided for quality control of products such as medicines, health product and the like of the polysaccharides.

The invention discloses a fluorescence quantitative PCR detection method for type and composition of yak muscle fibers. According to the fluorescence quantitative PCR detection method, yak muscle tissues are taken for total RNA extraction, the RNA purity and concentration are detected, the total RNA is subjected to inverse transcription to generate cDNA, then quality detection is carried out by taking GAPDH genes of yak as internal genes, and then a special detection primer is adopted for carrying out fluorescence quantitative PCR reaction, meanwhile, the GAPDH genes of yak are taken as reference genes for statistic analysis. The detection method and the detection primer provided by the invention are more accurate and more reliable than histochemistry evaluation; with the molecule quantitative classification method, accurate quantitative and analysis can be carried out on the type composition of the various muscle fibers in muscles at different parts of the yak, so that a technological base is laid for further study on the relevance between the muscle fibers and the meat quality trait of the yak, and meanwhile, compared with other methods, the fluorescence quantitative PCR detection method has the advantages of low workload and low cost, and high efficiency.

The invention provides a method for quantitative determination of uronic acid-containing polysaccharide. The method comprises the following specific steps of preparing a uronic acid-containing polysaccharide standard substance solution from trifluoroacetic acid, wherein the uronic acid-containing polysaccharide is the polysaccharide of which a main chain is formed by a hexuronic acid-hexosamine or hexuronic acid-hexose disaccharide repeated disaccharide fragment; carrying out acid hydrolysis and deacidification on a standard substance to obtain a to-be-tested sample acid hydrolysis residue; dissolving a standard sample acid hydrolysis residue, and carrying out analysis by using a high-performance liquid chromatography-tandem triple quadrupole mass spectrometer to obtain a multi-response monitoring chromatogram and a standard curve; processing and detecting the to-be-tested sample acid hydrolysis residue by adopting the same method; and identifying the variety of the uronic acid-containing polysaccharide and obtaining the content of the uronic acid-containing polysaccharide in the to-be-tested sample. The method is simple in operation step, low in reagent price and relatively short in elapsed time, and polysaccharide formed by repeated disaccharide fragments of uronic acid and hexosamine or hexose can be accurately quantified at the same time.

A positive-pressure automatic spume proportionality mixer systems includes pressure water pipeline, spume pipeline and mixer pipeline. There are at least spume tank, spume purification apparatus, ram-type pump, spume application valve ball and back valve setup at the spume pipeline with spume tank at the beginning, the out spout pipeline of back valve joins with pressure water pipeline to mixer pipeline. A flow rate sensor is set at the aforementioned mixer pipeline and connects to control module with wires, the control module connects with parameter input and display module and machine controller connecting with electric motor drive ram-type pump. The system can implement whole automatic control spume mixing proportion with simple operation, accurate adjustment, stabilizing and reliable operation activity.

The invention discloses a device for simulating emission of greenhouse gas of a drainage channel under an intermittent hydrodynamic condition and an experimental method thereof. The device comprises arice field system, a channel water tank, a water-bottom mud collecting system, a gas collecting system, a water supply system, a tail door and a water discharging groove; the water supply system is located on the side surface of the rice field system and is connected through a water channel pipeline; a rectangular plastic basin bowl in the rice field system is connected with the channel water tank at one side of a field ridge; the rice field system is used for realizing irrigation through the water supply system; water discharge to the channel water tank is realized through the field ridge; the gas collecting system and the water-bottom mud collecting system are arranged in the channel water tank at equal distances; the tail door is arranged at the tail part of the water tank, and the back part of the tail door is connected with a drainage groove; the greenhouse gases with different space depths in the drainage channel and the water and the bottom mud are sampled together in the controllable intermittent hydrodynamic condition, and the device has an important theory and practical significance for accurately measuring the emission flux and concentration distribution of greenhouse gas in the drainage channel of the rice field, disclosing the influence mechanism and the control factors.

The invention discloses a living body spontaneous activity quantitative detection device which comprises a box body structure with an upper layer and a lower layer, wherein the upper layer is a sensor layer which comprises infrared geminate transistors arranged in a 16*8 matrix, spacing between every two adjacent infrared geminate transistors is 3cm, infrared ray transmitting tubes and infrared ray receiving tubes of the infrared geminate transistors are arranged in parallel, a transparent plate is arranged above the infrared geminate transistors, the lower layer is a control plate layer which comprises a scanning circuit and a control circuit, the scanning circuit and the control circuit are electronically connected, the scanning circuit comprises 18 pieces of CD4051, the control circuit mainly comprises an ATMEGA16 processor chip, the infrared geminate transistors is connected with the scanning circuit through a transmission line, and the control circuit is connected with a display module outside the box body structure. The invention further discloses a detection method with the device used. According to the method, automatic detection of the whole process of the activity of a mouse can be achieved comprehensively and accurately with a simple method, accurate reflection and continuous detection of the process of the activity of the mouse are achieved, and accurate quantitative description of activity in two-dimensional space is achieved.

The present invention provides genetic marker, method and reagent kit for detecting ox originated / sheep originated components in feed. Specifically, the present invention provides the D-loop doze nucleotide sequence of the ox originated / sheep originated components in feed and the primer and probe designed based on the sequence. The method may be used in convenient, fast and accurate qualitative and quantitative detection of ox originated / sheep originated components in feed.

The present invention provides, among other things, methods of quantitating mRNA capping efficiency, particularly mRNA synthesized in vitro. In some embodiments, methods according to the present invention comprise providing an mRNA sample containing capped and uncapped mRNA, providing a cap specific binding substance under conditions that permit the formation of a complex between the cap specific binding substance and the capped mRNA, and quantitatively determining the amount of the complex as compared to a control, thereby quantifying mRNA capping efficiency.

The invention belongs to the technical field of chromatographic detection, and specifically relates to a method for quickly detecting vancomycin by utilizing LC-MS (Liquid Chromatography-Mass Spectrometry). The method comprises the following steps: smashing a to-be-detected sample, adding an extracting reagent, and uniformly mixing; then carrying out ultrasonic extraction, and centrifuging, thus obtaining supernatant; extracting the supernatant by adopting water saturated normal hexane, thus obtaining a to-be-purified solution; activating through an MCX solid-phase extraction column, taking and sampling the to-be-purified solution, carrying out drip washing and impurity removing by using methanol, then eluting by using amonium methanol, and collecting an eluent; concentrating, forming a constant volume by using a constant volume solution, and filtering, thus obtaining a purified solution; detecting the purified solution by adopting an HPLC-MS (High Performance Liquid Chromatograph-MassSpectrometer). The method disclosed by the invention has the advantages that the problems of large matrix effects, low sensitivity and the like existing in a process of detecting animal derived foodand particularly the animal derived food with a high fat content in the prior art are well solved, ideal recovery and precision degree are obtained, the minimum detection limit of the method is 0.3 mug / kg, and repeatability and reproducibility of an analysis result are effectively ensured.

The invention provides a method for quantitatively measuring the rusting ratio of the surface of a metal material, and the method includes the following steps: obtaining a local or total surface image of a to-be-measured sample through image acquisition equipment and inputting the image in a computer; opening the surface image of the to-be-measured image through phase area content measuring software of an automatic metallographical image analyzer; selecting a regional image required for measurement in the surface image of the to-be-measured sample; performing binaryzation treatment on the regional image, so as to convert the regional image into a two-value image only containing two colors; converting colors of all the rust regions in the regional image into one color of the two colors through adjusting the range of the threshold; extracting regions with the color representing the rust regions in the two-value image of the regional image; and calculating the percentage of the total area of the regions with the color representing the rust regions and the area of the regional image through the content measuring function of the phase area content measuring software, so as to obtain the rusting ratio of the surface of the to-be-measured sample. The method provided by the invention can be used for accurately measuring the rusting ratio of the surface of the metal material sample, and is simple, convenient and quick.

The invention discloses a separation and determination method for the mobility of nicotine optical isomers in cigarettes. The method comprises the following steps of: (1) collecting nicotine optical isomers in cigarettes; 2, collecting nicotine optical isomers in cigarette aerosol; (3) carrying out accurate quantitative determination on S-(-)-nicotine and R-(+)-nicotine; and (4) calculating the mobility of the nicotine optical isomers in the cigarettes. According to the separation and determination method, a Trap column is adopted for the first time to replace a quantitative loop, so that a target object cut by a one-dimensional column can be completely collected, and quantitative determination is more accurate; it is found for the first time that the mobility of R-(+)-nicotine to aerosolin traditional cigarettes is obviously higher than that of R-(+)-nicotine to aerosol in heated cigarettes.